Restriction map for
pF1KE0745
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MroI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tru9I Tsp509I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 564 GTCTAC GT^[AC][GT]AC
AfaI 207 GTAC GT^AC
AflIII 748 ACGCGT A^C[GA][CT]GT
ApaI 526 GGGCCC GGGCC^C
Asp700I 306 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 370 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 98 GGATCC G^GATCC
BcgI 870 CGAGGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 516 GGATAC GTATCC(6/5)
BfaI 598 CTAG C^TAG
BpmI 8 CTGGAG CTGGAG(16/14)
BsgI 607 GTGCAG GTGCAG(16/14)
BspMI 761 ACCTGC ACCTGC(4/8)
BsrDI 888 CATTGC GCAATG(2/0)
BsrFI 603 GCCGGT [GA]^CCGG[CT]
BstBI 21 TTCGAA TT^CGAA
BstYI 98 GGATCC [GA]^GATC[CT]
Cfr10I 603 GCCGGT [GA]^CCGG[CT]
DrdI 67 GACAAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 672 CCGCGG C^C[GA][CT]GG
EarI 772 CTCTTC CTCTTC(1/4)
EcoNI 807 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I 772 CTCTTC CTCTTC(1/4)
KspI 672 CCGCGG CCGC^GG
MaeI 598 CTAG C^TAG
MflI 98 GGATCC [GA]^GATC[CT]
MluI 748 ACGCGT A^CGCGT
NspV 21 TTCGAA TT^CGAA
PflFI 370 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 679 GAGTC GAGTC(4/5)
PstI 763 CTGCAG CTGCA^G
PvuII 553 CAGCTG CAG^CTG
RsaI 207 GTAC GT^AC
SacII 672 CCGCGG CCGC^GG
SapI 771 GCTCTTC GCTCTTC(1/4)
SbfI 762 CCTGCAGG CCTGCA^GG
SexAI 41 ACCAGGT A^CC[AT]GGT
SfcI 763 CTGCAG C^T[GA][CT]AG
SfuI 21 TTCGAA TT^CGAA
Sse8387I 762 CCTGCAGG CCTGCA^GG
TfiI 19 GATTC G^A[AT]TC
Tth111I 370 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 98 GGATCC [GA]^GATC[CT]
XmnI 306 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 99 GATCC GGATC(4/5)
98 GGATC
BsaI 262 GAGACC GGTCTC(1/5)
754 GAGACC
CfoI 654 GCGC GCG^C
880 GCGC
DdeI 321 CTCAG C^T[ACGT]AG
812 CTAAG
DpnII 99 GATC ^GATC
459 GATC
EcoT14I 167 CCAAGG C^C[AT][AT]GG
836 CCAAGG
HhaI 654 GCGC GCG^C
880 GCGC
HinP1I 654 GCGC G^CGC
880 GCGC
HinfI 19 GATTC G^A[ACGT]TC
679 GAGTC
MaeII 385 ACGT A^CGT
932 ACGT
MboI 99 GATC ^GATC
459 GATC
NdeII 99 GATC ^GATC
459 GATC
NlaIII 531 CATG CATG^
684 CATG
PpuMI 404 AGGACCT [GA]G^G[AT]CC[CT]
428 AGGACCC
Sau3AI 99 GATC ^GATC
459 GATC
SmlI 91 CTCAAG C^T[CT][GA]AG
445 CTCAAG
StyI 167 CCAAGG C^C[AT][AT]GG
836 CCAAGG
TaqI 22 TCGA T^CGA
935 TCGA
TthHB8I 22 TCGA T^CGA
935 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BalI BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeIII MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV SacI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI