################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 54 CGCG CG^CG AcsI 274 AAATTC [GA]^AATT[CT] AluI 237 AGCT AG^CT AlwI 303 GATCC GGATC(4/5) Aor51HI 362 AGCGCT AGC^GCT ApaI 115 GGGCCC GGGCC^C ApoI 274 AAATTC [GA]^AATT[CT] BalI 419 TGGCCA TGG^CCA BanII 115 GGGCCC G[GA]GC[CT]^C BbsI 64 GTCTTC GAAGAC(2/6) BclI 590 TGATCA T^GATCA BpuAI 64 GTCTTC GAAGAC(2/6) BsaI 187 GGTCTC GGTCTC(1/5) BsiEI 95 CGGCCG CG[GA][CT]^CG BsiYI 48 CCTCATCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 48 CCTCATCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 84 GGGAC GGGAC(10/14) BspHI 299 TCATGA T^CATGA BsrI 450 CCAGT ACTGG(1/-1) BstF5I 585 GGATG GGATG(2/0) BstUI 54 CGCG CG^CG DdeI 234 CTTAG C^T[ACGT]AG EagI 95 CGGCCG C^GGCCG EarI 190 CTCTTC CTCTTC(1/4) EclXI 95 CGGCCG C^GGCCG Eco47III 362 AGCGCT AGC^GCT Eco52I 95 CGGCCG C^GGCCG EcoT14I 641 CCAAGG C^C[AT][AT]GG FauI 55 GCGGG CCCGC(4/6) FbaI 590 TGATCA T^GATCA FokI 585 GGATG GGATG(9/13) HaeII 362 AGCGCT [GA]GCGC^[CT] Ksp632I 190 CTCTTC CTCTTC(1/4) MaeIII 124 GTCAC ^GT[ACGT]AC MluNI 419 TGGCCA TGG^CCA MscI 419 TGGCCA TGG^CCA MvnI 54 CGCG CG^CG NspI 495 GCATGT [GA]CATG^[CT] PpuMI 84 GGGACCT [GA]G^G[AT]CC[CT] RcaI 299 TCATGA T^CATGA SfaNI 350 GCATC GCATC(5/9) SfcI 239 CTACAG C^T[GA][CT]AG StyI 641 CCAAGG C^C[AT][AT]GG TaqI 614 TCGA T^CGA Tsp45I 124 GTCAC ^GT[GC]AC TspRI 215 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 614 TCGA T^CGA XbaI 516 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AspHI 31 GTGCTC G[AT]GC[AT]^C 549 GAGCAC AvaII 59 GGTCC G^G[AT]CC 85 GGACC BfaI 484 CTAG C^TAG 517 CTAG BsiHKAI 31 GTGCTC G[AT]GC[AT]^C 549 GAGCAC BsmAI 314 GAGAC GTCTC(1/5) 188 GTCTC Cac8I 427 GCAGGC GC[ACGT]^[ACGT]GC 491 GCAAGC CfoI 6 GCGC GCG^C 363 GCGC DraII 84 GGGACCT [GA]G^G[ACGT]CC[CT] 114 GGGGCCC EaeI 95 CGGCCG [CT]^GGCC[GA] 419 TGGCCA EcoO109I 84 GGGACCT [GA]G^G[ACGT]CC[CT] 114 GGGGCCC HhaI 6 GCGC GCG^C 363 GCGC HinP1I 6 GCGC G^CGC 363 GCGC MaeI 484 CTAG C^TAG 517 CTAG MslI 24 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 525 CATGGAGGTG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI BbvI BcnI BmyI BsaJI Bsp1286I BssKI BstNI Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp509I