Restriction map for pF1KB6971
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        Eam1105I    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MflI        MluI        MroI        MseI        MspA1I      
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tth111I     Van91I      XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII          54  CGCG                 CG^CG
AcsI          274  AAATTC               [GA]^AATT[CT]
AluI          237  AGCT                 AG^CT
AlwI          303  GATCC                GGATC(4/5)
Aor51HI       362  AGCGCT               AGC^GCT
ApaI          115  GGGCCC               GGGCC^C
ApoI          274  AAATTC               [GA]^AATT[CT]
BalI          419  TGGCCA               TGG^CCA
BanII         115  GGGCCC               G[GA]GC[CT]^C
BbsI           64  GTCTTC               GAAGAC(2/6)
BclI          590  TGATCA               T^GATCA
BpuAI          64  GTCTTC               GAAGAC(2/6)
BsaI          187  GGTCTC               GGTCTC(1/5)
BsiEI          95  CGGCCG               CG[GA][CT]^CG
BsiYI          48  CCTCATCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           48  CCTCATCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          84  GGGAC                GGGAC(10/14)
BspHI         299  TCATGA               T^CATGA
BsrI          450  CCAGT                ACTGG(1/-1)
BstF5I        585  GGATG                GGATG(2/0)
BstUI          54  CGCG                 CG^CG
DdeI          234  CTTAG                C^T[ACGT]AG
EagI           95  CGGCCG               C^GGCCG
EarI          190  CTCTTC               CTCTTC(1/4)
EclXI          95  CGGCCG               C^GGCCG
Eco47III      362  AGCGCT               AGC^GCT
Eco52I         95  CGGCCG               C^GGCCG
EcoT14I       641  CCAAGG               C^C[AT][AT]GG
FauI           55  GCGGG                CCCGC(4/6)
FbaI          590  TGATCA               T^GATCA
FokI          585  GGATG                GGATG(9/13)
HaeII         362  AGCGCT               [GA]GCGC^[CT]
Ksp632I       190  CTCTTC               CTCTTC(1/4)
MaeIII        124  GTCAC                ^GT[ACGT]AC
MluNI         419  TGGCCA               TGG^CCA
MscI          419  TGGCCA               TGG^CCA
MvnI           54  CGCG                 CG^CG
NspI          495  GCATGT               [GA]CATG^[CT]
PpuMI          84  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          299  TCATGA               T^CATGA
SfaNI         350  GCATC                GCATC(5/9)
SfcI          239  CTACAG               C^T[GA][CT]AG
StyI          641  CCAAGG               C^C[AT][AT]GG
TaqI          614  TCGA                 T^CGA
Tsp45I        124  GTCAC                ^GT[GC]AC
TspRI         215  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       614  TCGA                 T^CGA
XbaI          516  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AspHI          31  GTGCTC               G[AT]GC[AT]^C
              549  GAGCAC              
AvaII          59  GGTCC                G^G[AT]CC
               85  GGACC               
BfaI          484  CTAG                 C^TAG
              517  CTAG                
BsiHKAI        31  GTGCTC               G[AT]GC[AT]^C
              549  GAGCAC              
BsmAI         314  GAGAC                GTCTC(1/5)
              188  GTCTC               
Cac8I         427  GCAGGC               GC[ACGT]^[ACGT]GC
              491  GCAAGC              
CfoI            6  GCGC                 GCG^C
              363  GCGC                
DraII          84  GGGACCT              [GA]G^G[ACGT]CC[CT]
              114  GGGGCCC             
EaeI           95  CGGCCG               [CT]^GGCC[GA]
              419  TGGCCA              
EcoO109I       84  GGGACCT              [GA]G^G[ACGT]CC[CT]
              114  GGGGCCC             
HhaI            6  GCGC                 GCG^C
              363  GCGC                
HinP1I          6  GCGC                 G^CGC
              363  GCGC                
MaeI          484  CTAG                 C^TAG
              517  CTAG                
MslI           24  CATGAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              525  CATGGAGGTG          


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        BbvI        BcnI        BmyI        BsaJI       
Bsp1286I    BssKI       BstNI       Cfr13I      DpnII       
EcoRII      Fnu4HI      HaeIII      HapII       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
TseI        Tsp509I     
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