Restriction map for
pF1KB6971
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MseI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 54 CGCG CG^CG
AcsI 274 AAATTC [GA]^AATT[CT]
AluI 237 AGCT AG^CT
AlwI 303 GATCC GGATC(4/5)
Aor51HI 362 AGCGCT AGC^GCT
ApaI 115 GGGCCC GGGCC^C
ApoI 274 AAATTC [GA]^AATT[CT]
BalI 419 TGGCCA TGG^CCA
BanII 115 GGGCCC G[GA]GC[CT]^C
BbsI 64 GTCTTC GAAGAC(2/6)
BclI 590 TGATCA T^GATCA
BpuAI 64 GTCTTC GAAGAC(2/6)
BsaI 187 GGTCTC GGTCTC(1/5)
BsiEI 95 CGGCCG CG[GA][CT]^CG
BsiYI 48 CCTCATCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 48 CCTCATCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 84 GGGAC GGGAC(10/14)
BspHI 299 TCATGA T^CATGA
BsrI 450 CCAGT ACTGG(1/-1)
BstF5I 585 GGATG GGATG(2/0)
BstUI 54 CGCG CG^CG
DdeI 234 CTTAG C^T[ACGT]AG
EagI 95 CGGCCG C^GGCCG
EarI 190 CTCTTC CTCTTC(1/4)
EclXI 95 CGGCCG C^GGCCG
Eco47III 362 AGCGCT AGC^GCT
Eco52I 95 CGGCCG C^GGCCG
EcoT14I 641 CCAAGG C^C[AT][AT]GG
FauI 55 GCGGG CCCGC(4/6)
FbaI 590 TGATCA T^GATCA
FokI 585 GGATG GGATG(9/13)
HaeII 362 AGCGCT [GA]GCGC^[CT]
Ksp632I 190 CTCTTC CTCTTC(1/4)
MaeIII 124 GTCAC ^GT[ACGT]AC
MluNI 419 TGGCCA TGG^CCA
MscI 419 TGGCCA TGG^CCA
MvnI 54 CGCG CG^CG
NspI 495 GCATGT [GA]CATG^[CT]
PpuMI 84 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 299 TCATGA T^CATGA
SfaNI 350 GCATC GCATC(5/9)
SfcI 239 CTACAG C^T[GA][CT]AG
StyI 641 CCAAGG C^C[AT][AT]GG
TaqI 614 TCGA T^CGA
Tsp45I 124 GTCAC ^GT[GC]AC
TspRI 215 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 614 TCGA T^CGA
XbaI 516 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AspHI 31 GTGCTC G[AT]GC[AT]^C
549 GAGCAC
AvaII 59 GGTCC G^G[AT]CC
85 GGACC
BfaI 484 CTAG C^TAG
517 CTAG
BsiHKAI 31 GTGCTC G[AT]GC[AT]^C
549 GAGCAC
BsmAI 314 GAGAC GTCTC(1/5)
188 GTCTC
Cac8I 427 GCAGGC GC[ACGT]^[ACGT]GC
491 GCAAGC
CfoI 6 GCGC GCG^C
363 GCGC
DraII 84 GGGACCT [GA]G^G[ACGT]CC[CT]
114 GGGGCCC
EaeI 95 CGGCCG [CT]^GGCC[GA]
419 TGGCCA
EcoO109I 84 GGGACCT [GA]G^G[ACGT]CC[CT]
114 GGGGCCC
HhaI 6 GCGC GCG^C
363 GCGC
HinP1I 6 GCGC G^CGC
363 GCGC
MaeI 484 CTAG C^TAG
517 CTAG
MslI 24 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
525 CATGGAGGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI BbvI BcnI BmyI BsaJI
Bsp1286I BssKI BstNI Cfr13I DpnII
EcoRII Fnu4HI HaeIII HapII HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp509I