Restriction map for
pF1KB6681
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BanI BbeI BbrPI BcgI
BciVI BclI BfrI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII Eam1105I EarI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SfcI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 939 CGCG CG^CG
AcsI 376 AAATTC [GA]^AATT[CT]
Aor51HI 869 AGCGCT AGC^GCT
ApoI 376 AAATTC [GA]^AATT[CT]
AspHI 692 GAGCAC G[AT]GC[AT]^C
AspI 412 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 563 GGATCC G^GATCC
BbsI 270 GAAGAC GAAGAC(2/6)
BbvCI 964 GCTGAGG CCTCAGC(-5/-2)
BglII 356 AGATCT A^GATCT
BpuAI 270 GAAGAC GAAGAC(2/6)
BsaI 36 GGTCTC GGTCTC(1/5)
BsiEI 245 CGGCCG CG[GA][CT]^CG
BsiHKAI 692 GAGCAC G[AT]GC[AT]^C
BsmBI 304 GAGACG CGTCTC(1/5)
BsrBI 778 GAGCGG CCGCTC(-3/-3)
BstAPI 753 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 939 CGCG CG^CG
DrdI 670 GACATCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 245 CGGCCG C^GGCCG
EclXI 245 CGGCCG C^GGCCG
Eco47III 869 AGCGCT AGC^GCT
Eco52I 245 CGGCCG C^GGCCG
EcoNI 888 CCTAGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 940 GCGTC GACGC(5/10)
HphI 1254 GGTGA GGTGA(8/7)
MaeII 307 ACGT A^CGT
MvnI 939 CGCG CG^CG
NotI 244 GCGGCCGC GC^GGCCGC
NspI 1064 ACATGC [GA]CATG^[CT]
PflFI 412 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 786 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1229 GACTC GAGTC(4/5)
PpuMI 179 GGGTCCC [GA]G^G[AT]CC[CT]
ScaI 1188 AGTACT AGT^ACT
SexAI 413 ACCTGGT A^CC[AT]GGT
SfiI 327 GGCCATCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 842 CCCGGG CCC^GGG
SmlI 509 CTCAAG C^T[CT][GA]AG
StuI 968 AGGCCT AGG^CCT
TfiI 1257 GAATC G^A[AT]TC
Tth111I 412 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 786 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 842 CCCGGG C^CCGGG
XmaI 842 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 191 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
604 CAGTACCTG
ApaI 782 GGGCCC GGGCC^C
983 GGGCCC
BfaI 889 CTAG C^TAG
1072 CTAG
BlpI 1003 GCTGAGC GC^T[ACGT]AGC
1054 GCTCAGC
Bpu1102I 1003 GCTGAGC GC^T[ACGT]AGC
1054 GCTCAGC
BsmAI 304 GAGAC GTCTC(1/5)
37 GTCTC
BsrDI 201 CATTGC GCAATG(2/0)
294 CATTGC
BstF5I 102 CATCC GGATG(2/0)
950 GGATG
CelII 1003 GCTGAGC GC^T[ACGT]AGC
1054 GCTCAGC
CfoI 870 GCGC GCG^C
885 GCGC
DraII 179 GGGTCCC [GA]G^G[ACGT]CC[CT]
982 GGGGCCC
EcoO109I 179 GGGTCCC [GA]G^G[ACGT]CC[CT]
982 GGGGCCC
FokI 102 CATCC GGATG(9/13)
950 GGATG
HaeII 869 AGCGCT [GA]GCGC^[CT]
884 AGCGCC
HhaI 870 GCGC GCG^C
885 GCGC
HinP1I 870 GCGC G^CGC
885 GCGC
HincII 418 GTCAAC GT[CT]^[GA]AC
679 GTCAAC
HindII 418 GTCAAC GT[CT]^[GA]AC
679 GTCAAC
HinfI 1229 GACTC G^A[ACGT]TC
1257 GAATC
MaeI 889 CTAG C^TAG
1072 CTAG
MaeIII 193 GTCAC ^GT[ACGT]AC
1177 GTGAC
MboII 270 GAAGA GAAGA(8/7)
654 GAAGA
PvuII 1036 CAGCTG CAG^CTG
1057 CAGCTG
Tsp45I 193 GTCAC ^GT[GC]AC
1177 GTGAC
Tsp509I 377 AATT ^AATT
915 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HpaII ItaI
MboI MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I
XhoII