Restriction map for
pF1KB9951
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanII BbeI BbrPI
BcgI BclI BcnI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MluI MroI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI SspBI StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 1174 AAATTC [GA]^AATT[CT]
AflIII 455 ACATGT A^C[GA][CT]GT
ApoI 1174 AAATTC [GA]^AATT[CT]
AvaI 1459 CCCGAG C^[CT]CG[GA]G
AvaII 1114 GGACC G^G[AT]CC
BalI 251 TGGCCA TGG^CCA
BanI 1421 GGCACC G^G[CT][GA]CC
BlpI 1471 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1471 GCTGAGC GC^T[ACGT]AGC
BsaI 1537 GAGACC GGTCTC(1/5)
BsmBI 1596 CGTCTC CGTCTC(1/5)
BsoBI 1459 CCCGAG C^[CT]CG[GA]G
BspLU11I 455 ACATGT A^CATGT
BspMI 569 ACCTGC ACCTGC(4/8)
BsrDI 827 GCAATG GCAATG(2/0)
BstXI 1070 CCAAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1199 GGATCT [GA]^GATC[CT]
CelII 1471 GCTGAGC GC^T[ACGT]AGC
CfoI 186 GCGC GCG^C
DrdI 81 GACATCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 251 TGGCCA [CT]^GGCC[GA]
HaeII 185 GGCGCT [GA]GCGC^[CT]
HhaI 186 GCGC GCG^C
HinP1I 186 GCGC G^CGC
HincII 1518 GTTGAC GT[CT]^[GA]AC
HindII 1518 GTTGAC GT[CT]^[GA]AC
MfeI 367 CAATTG C^AATTG
MflI 1199 GGATCT [GA]^GATC[CT]
MluNI 251 TGGCCA TGG^CCA
MscI 251 TGGCCA TGG^CCA
MunI 367 CAATTG C^AATTG
NdeI 842 CATATG CA^TATG
PflMI 733 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 703 GAAGAGC GCTCTTC(1/4)
SbfI 1108 CCTGCAGG CCTGCA^GG
ScaI 24 AGTACT AGT^ACT
SexAI 228 ACCAGGT A^CC[AT]GGT
SpeI 1084 ACTAGT A^CTAGT
Sse8387I 1108 CCTGCAGG CCTGCA^GG
SspI 401 AATATT AAT^ATT
TaqI 699 TCGA T^CGA
TthHB8I 699 TCGA T^CGA
Van91I 733 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1199 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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Alw44I 540 GTGCAC G^TGCAC
794 GTGCAC
AlwI 1041 GATCC GGATC(4/5)
1199 GGATC
AlwNI 68 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1583 CAGCCGCTG
ApaLI 540 GTGCAC G^TGCAC
794 GTGCAC
AspHI 540 GTGCAC G[AT]GC[AT]^C
794 GTGCAC
BbvCI 113 CCTCAGC CCTCAGC(-5/-2)
1309 GCTGAGG
BciVI 258 GGATAC GTATCC(6/5)
1080 GGATAC
BglI 177 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1090 GCCGTACTGGC
BpmI 799 CTGGAG CTGGAG(16/14)
814 CTGGAG
BsgI 571 CTGCAC GTGCAG(16/14)
1480 GTGCAG
BsiHKAI 540 GTGCAC G[AT]GC[AT]^C
794 GTGCAC
BsmFI 1026 GGGAC GGGAC(10/14)
15 GTCCC
BsrFI 1418 ACCGGC [GA]^CCGG[CT]
1514 GCCGGT
Cfr10I 1418 ACCGGC [GA]^CCGG[CT]
1514 GCCGGT
DsaI 254 CCATGG C^C[GA][CT]GG
597 CCATGG
EcoT14I 254 CCATGG C^C[AT][AT]GG
597 CCATGG
HapII 1419 CCGG C^CGG
1515 CCGG
HpaII 1419 CCGG C^CGG
1515 CCGG
MslI 574 CACTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
750 CACTAATGTG
MspI 1419 CCGG C^CGG
1515 CCGG
NcoI 254 CCATGG C^CATGG
597 CCATGG
NspI 455 ACATGT [GA]CATG^[CT]
788 GCATGT
StyI 254 CCATGG C^C[AT][AT]GG
597 CCATGG
XbaI 882 TCTAGA T^CTAGA
1056 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI Asp700I BbsI
BbvI BfaI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI