Restriction map for
pF1KB8616
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# NO CUT ENZYMES ( 140 enzymes) #
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AccI AccIII AclI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspI AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
HapII HgaI HincII HindII HindIII
HpaI HpaII KasI MamI MluI
MluNI MroI MscI MspI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 398 GACGTC GACGT^C
Acc65I 483 GGTACC G^GTACC
AcyI 398 GACGTC G[GA]^CG[CT]C
AhdI 1081 GACGATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 593 GGATC GGATC(4/5)
ApaI 456 GGGCCC GGGCC^C
Asp718I 483 GGTACC G^GTACC
AspEI 1081 GACGATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3 GTGCTC G[AT]GC[AT]^C
AvaI 37 CCCGAG C^[CT]CG[GA]G
AviII 104 TGCGCA TGC^GCA
BanI 483 GGTACC G^G[CT][GA]CC
BbsI 1044 GTCTTC GAAGAC(2/6)
BglII 149 AGATCT A^GATCT
BpuAI 1044 GTCTTC GAAGAC(2/6)
BsaHI 398 GACGTC G[GA]^CG[CT]C
BsaI 806 GGTCTC GGTCTC(1/5)
BseRI 961 GAGGAG GAGGAG(10/8)
BsiHKAI 3 GTGCTC G[AT]GC[AT]^C
BsiYI 133 CCCGACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 133 CCCGACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 37 CCCGAG C^[CT]CG[GA]G
BsrBI 826 GAGCGG CCGCTC(-3/-3)
BstYI 149 AGATCT [GA]^GATC[CT]
DraI 745 TTTAAA TTT^AAA
DraII 455 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 392 GACTTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1081 GACGATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 455 AGGGCCC [GA]G^G[ACGT]CC[CT]
FspI 104 TGCGCA TGC^GCA
HaeII 86 AGCGCC [GA]GCGC^[CT]
Hin1I 398 GACGTC G[GA]^CG[CT]C
KpnI 483 GGTACC GGTAC^C
MaeII 399 ACGT A^CGT
MfeI 263 CAATTG C^AATTG
MflI 149 AGATCT [GA]^GATC[CT]
MunI 263 CAATTG C^AATTG
NdeI 892 CATATG CA^TATG
NlaIII 693 CATG CATG^
SapI 823 GAAGAGC GCTCTTC(1/4)
ScaI 640 AGTACT AGT^ACT
SfcI 350 CTGTAG C^T[GA][CT]AG
SpeI 330 ACTAGT A^CTAGT
SspI 1009 AATATT AAT^ATT
StuI 657 AGGCCT AGG^CCT
XhoII 149 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 16 CGCG CG^CG
518 CGCG
AvaII 606 GGACC G^G[AT]CC
1088 GGTCC
BanII 25 GAGCCC G[GA]GC[CT]^C
456 GGGCCC
BsmFI 390 GGGAC GGGAC(10/14)
670 GGGAC
BsrI 665 CCAGT ACTGG(1/-1)
724 CCAGT
BstF5I 340 GGATG GGATG(2/0)
1071 GGATG
BstUI 16 CGCG CG^CG
518 CGCG
BstXI 387 CCAGGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
795 CCAGAAGAGTGG
DpnII 150 GATC ^GATC
594 GATC
DsaI 15 CCGCGG C^C[GA][CT]GG
517 CCGCGG
EcoRI 300 GAATTC G^AATTC
1099 GAATTC
EcoT14I 609 CCAAGG C^C[AT][AT]GG
926 CCTTGG
FokI 340 GGATG GGATG(9/13)
1071 GGATG
KspI 15 CCGCGG CCGC^GG
517 CCGCGG
MaeIII 585 GTTAC ^GT[ACGT]AC
759 GTTAC
MboI 150 GATC ^GATC
594 GATC
MslI 693 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
850 CATCTTGATG
MvnI 16 CGCG CG^CG
518 CGCG
NdeII 150 GATC ^GATC
594 GATC
NlaIV 456 GGGCCC GG[ACGT]^[ACGT]CC
483 GGTACC
PleI 157 GACTC GAGTC(4/5)
1042 GAGTC
PvuII 733 CAGCTG CAG^CTG
904 CAGCTG
SacII 15 CCGCGG CCGC^GG
517 CCGCGG
Sau3AI 150 GATC ^GATC
594 GATC
StyI 609 CCAAGG C^C[AT][AT]GG
926 CCTTGG
TaqI 23 TCGA T^CGA
768 TCGA
TfiI 778 GATTC G^A[AT]TC
922 GAATC
TthHB8I 23 TCGA T^CGA
768 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BmyI BsaJI BsmAI
Bsp1286I BsrDI BssKI BstNI Cac8I
CfoI Cfr13I DdeI EarI EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MboII MnlI MseI MspA1I MvaI
MwoI RsaI Sau96I ScrFI SfaNI
Tru9I TseI Tsp509I TspRI