Restriction map for
pF1KB3580
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AspHI AvaI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspEI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT14I FbaI FseI HaeII
HgaI Hin1I HpaI HphI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StyI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AlwNI 503 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1350 CCTGAGG CC^T[ACGT]AGG
ApaI 827 GGGCCC GGGCC^C
AseI 214 ATTAAT AT^TAAT
AsnI 214 ATTAAT AT^TAAT
AspI 613 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 71 TGCGCA TGC^GCA
BalI 383 TGGCCA TGG^CCA
BanI 231 GGCACC G^G[CT][GA]CC
BbsI 1525 GAAGAC GAAGAC(2/6)
BcnI 686 CCCGG CC^[GC]GG
BfaI 1255 CTAG C^TAG
BglI 820 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1472 AGATCT A^GATCT
BpuAI 1525 GAAGAC GAAGAC(2/6)
BsgI 606 GTGCAG GTGCAG(16/14)
BsmAI 897 GAGAC GTCTC(1/5)
BsmI 690 GAATGC GAATGC(1/-1)
BspDI 877 ATCGAT AT^CGAT
BsrBI 1279 CCGCTC CCGCTC(-3/-3)
BstF5I 978 GGATG GGATG(2/0)
BstXI 386 CCAGGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1350 CCTGAGG CC^T[ACGT]AGG
CfoI 72 GCGC GCG^C
ClaI 877 ATCGAT AT^CGAT
DraII 827 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 233 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 383 TGGCCA [CT]^GGCC[GA]
Eco81I 1350 CCTGAGG CC^T[ACGT]AGG
EcoNI 1594 CCTATGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 827 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1083 GATATC GAT^ATC
EcoT22I 916 ATGCAT ATGCA^T
FauI 883 GCGGG CCCGC(4/6)
FokI 978 GGATG GGATG(9/13)
FspI 71 TGCGCA TGC^GCA
HapII 687 CCGG C^CGG
HhaI 72 GCGC GCG^C
HinP1I 72 GCGC G^CGC
HincII 1549 GTTGAC GT[CT]^[GA]AC
HindII 1549 GTTGAC GT[CT]^[GA]AC
HindIII 1421 AAGCTT A^AGCTT
HpaII 687 CCGG C^CGG
MaeI 1255 CTAG C^TAG
MluNI 383 TGGCCA TGG^CCA
MscI 383 TGGCCA TGG^CCA
MspI 687 CCGG C^CGG
NciI 686 CCCGG CC^[GC]GG
NsiI 916 ATGCAT ATGCA^T
NspI 1072 GCATGT [GA]CATG^[CT]
PflFI 613 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 214 ATTAAT AT^TAAT
SapI 121 GAAGAGC GCTCTTC(1/4)
SexAI 234 ACCAGGT A^CC[AT]GGT
StuI 1484 AGGCCT AGG^CCT
TaqI 878 TCGA T^CGA
Tth111I 613 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 878 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 1116 GATCC GGATC(4/5)
1231 GGATC
AvaII 850 GGACC G^G[AT]CC
1514 GGACC
BanII 572 GGGCTC G[GA]GC[CT]^C
827 GGGCCC
BlpI 531 GCTCAGC GC^T[ACGT]AGC
1010 GCTCAGC
Bpu1102I 531 GCTCAGC GC^T[ACGT]AGC
1010 GCTCAGC
BsaJI 9 CCCAGG C^C[ACGT][ACGT]GG
1544 CCCAGG
BseRI 1291 CTCCTC GAGGAG(10/8)
78 GAGGAG
BsmFI 611 GGGAC GGGAC(10/14)
1513 GGGAC
Bsp1407I 421 TGTACA T^GTACA
1317 TGTACA
BspHI 201 TCATGA T^CATGA
1458 TCATGA
BsrDI 585 CATTGC GCAATG(2/0)
1536 CATTGC
BsrGI 421 TGTACA T^GTACA
1317 TGTACA
BstYI 1115 AGATCC [GA]^GATC[CT]
1472 AGATCT
Cac8I 820 GCCTGC GC[ACGT]^[ACGT]GC
951 GCGAGC
CelII 531 GCTCAGC GC^T[ACGT]AGC
1010 GCTCAGC
EarI 476 CTCTTC CTCTTC(1/4)
121 GAAGAG
Ksp632I 476 CTCTTC CTCTTC(1/4)
121 GAAGAG
MflI 1115 AGATCC [GA]^GATC[CT]
1472 AGATCT
MslI 222 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1296 CACGGTCATG
MspA1I 1097 CAGCTG C[AC]G^C[GT]G
1235 CAGCTG
PleI 1306 GACTC GAGTC(4/5)
1455 GAGTC
PvuII 1097 CAGCTG CAG^CTG
1235 CAGCTG
RcaI 201 TCATGA T^CATGA
1458 TCATGA
SspBI 421 TGTACA T^GTACA
1317 TGTACA
SspI 457 AATATT AAT^ATT
1090 AATATT
Tsp45I 797 GTGAC ^GT[GC]AC
970 GTCAC
XhoII 1115 AGATCC [GA]^GATC[CT]
1472 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BmyI BpmI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI