Restriction map for
pF1KB6982
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I CelII
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeII MamI MluI
MluNI MroI MscI MslI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AlwNI 116 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 445 CCTCAGG CC^T[ACGT]AGG
AvaI 52 CCCGAG C^[CT]CG[GA]G
BfaI 724 CTAG C^TAG
BmyI 278 GGGCAC G[AGT]GC[ACT]^C
BsaWI 241 ACCGGA [AT]^CCGG[AT]
BsgI 140 GTGCAG GTGCAG(16/14)
BsiYI 53 CCGAGGGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 53 CCGAGGGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 734 GAGAC GTCTC(1/5)
BsmFI 3 GTCCC GGGAC(10/14)
BsmI 381 GAATGC GAATGC(1/-1)
BsoBI 52 CCCGAG C^[CT]CG[GA]G
Bsp1286I 278 GGGCAC G[AGT]GC[ACT]^C
BspMI 469 GCAGGT ACCTGC(4/8)
BsrFI 19 GCCGGT [GA]^CCGG[CT]
BsrI 299 ACTGG ACTGG(1/-1)
BstYI 262 AGATCC [GA]^GATC[CT]
Bsu36I 445 CCTCAGG CC^T[ACGT]AGG
Cac8I 280 GCACGC GC[ACGT]^[ACGT]GC
CfoI 17 GCGC GCG^C
Cfr10I 19 GCCGGT [GA]^CCGG[CT]
Cfr13I 442 GGCCC G^G[ACGT]CC
DraI 487 TTTAAA TTT^AAA
DraII 441 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 6 CCGTGG C^C[GA][CT]GG
EarI 433 GAAGAG CTCTTC(1/4)
Eco81I 445 CCTCAGG CC^T[ACGT]AGG
EcoO109I 441 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 502 GCGGG CCCGC(4/6)
HaeII 16 AGCGCC [GA]GCGC^[CT]
HhaI 17 GCGC GCG^C
HinP1I 17 GCGC G^CGC
HincII 522 GTCAAC GT[CT]^[GA]AC
HindII 522 GTCAAC GT[CT]^[GA]AC
Ksp632I 433 GAAGAG CTCTTC(1/4)
MaeI 724 CTAG C^TAG
MfeI 620 CAATTG C^AATTG
MflI 262 AGATCC [GA]^GATC[CT]
MunI 620 CAATTG C^AATTG
NlaIV 10 GGTTCC GG[ACGT]^[ACGT]CC
PvuII 251 CAGCTG CAG^CTG
SapI 433 GAAGAGC GCTCTTC(1/4)
Sau96I 442 GGCCC G^G[ACGT]CC
SbfI 466 CCTGCAGG CCTGCA^GG
SfaNI 706 GATGC GCATC(5/9)
Sse8387I 466 CCTGCAGG CCTGCA^GG
StuI 394 AGGCCT AGG^CCT
XhoII 262 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 370 GTCTAC GT^[AC][GT]AC
606 GTCTAC
AciI 30 CCGC CCGC(-3/-1)
502 GCGG
AcsI 556 AAATTT [GA]^AATT[CT]
676 AAATTC
AfaI 166 GTAC GT^AC
271 GTAC
AlwI 263 GATCC GGATC(4/5)
463 GATCC
ApoI 556 AAATTT [GA]^AATT[CT]
676 AAATTC
BbvI 292 GCAGC GCAGC(8/12)
499 GCAGC
BssKI 99 CCAGG ^CC[ACGT]GG
267 CCAGG
BstNI 99 CCAGG CC^[AT]GG
267 CCAGG
DpnII 263 GATC ^GATC
463 GATC
EcoRII 99 CCAGG ^CC[AT]GG
267 CCAGG
Fnu4HI 292 GCAGC GC^[ACGT]GC
499 GCAGC
HapII 20 CCGG C^CGG
242 CCGG
HpaII 20 CCGG C^CGG
242 CCGG
HphI 49 TCACC GGTGA(8/7)
711 TCACC
ItaI 292 GCAGC GC^[ACGT]GC
499 GCAGC
MboI 263 GATC ^GATC
463 GATC
MspA1I 251 CAGCTG C[AC]G^C[GT]G
500 CAGCGG
MspI 20 CCGG C^CGG
242 CCGG
MvaI 99 CCAGG CC^[AT]GG
267 CCAGG
MwoI 82 GCTTTTAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
493 GCCACTGCAGC
NdeII 263 GATC ^GATC
463 GATC
NlaIII 336 CATG CATG^
599 CATG
NspI 335 ACATGC [GA]CATG^[CT]
598 ACATGC
PleI 198 GACTC GAGTC(4/5)
520 GAGTC
PstI 467 CTGCAG CTGCA^G
497 CTGCAG
RsaI 166 GTAC GT^AC
271 GTAC
Sau3AI 263 GATC ^GATC
463 GATC
ScrFI 99 CCAGG CC^[ACGT]GG
267 CCAGG
SfcI 467 CTGCAG C^T[GA][CT]AG
497 CTGCAG
TseI 292 GCAGC G^C[AT]GC
499 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BsaJI DdeI HaeIII HinfI
MaeIII MboII MnlI MseI TfiI
Tru9I Tsp45I Tsp509I TspRI