Restriction map for
pF1KB5730
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# NO CUT ENZYMES ( 108 enzymes) #
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Acc65I AccI AccIII AclI AfaI
AflII AgeI AocI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbeI BbsI BclI BfrI
BglI BpuAI BsaBI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1612 GACGTC GACGT^C
AccII 1932 CGCG CG^CG
AcsI 408 GAATTC [GA]^AATT[CT]
AcyI 1612 GACGTC G[GA]^CG[CT]C
AflIII 1034 ACATGT A^C[GA][CT]GT
AhdI 943 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2150 GTGCAC G^TGCAC
ApaLI 2150 GTGCAC G^TGCAC
ApoI 408 GAATTC [GA]^AATT[CT]
AspEI 943 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1296 CCCGAG C^[CT]CG[GA]G
AvrII 630 CCTAGG C^CTAGG
BbrPI 2153 CACGTG CAC^GTG
BciVI 468 GGATAC GTATCC(6/5)
BlnI 630 CCTAGG C^CTAGG
BlpI 1161 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1161 GCTCAGC GC^T[ACGT]AGC
BsaAI 2153 CACGTG [CT]AC^GT[GA]
BsaHI 1612 GACGTC G[GA]^CG[CT]C
BsaWI 1535 TCCGGT [AT]^CCGG[AT]
BsgI 297 CTGCAC GTGCAG(16/14)
BsmI 1902 GAATGC GAATGC(1/-1)
BsoBI 1296 CCCGAG C^[CT]CG[GA]G
BspLU11I 1034 ACATGT A^CATGT
BstAPI 2152 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2021 TTCGAA TT^CGAA
BstEII 1110 GGTGACC G^GT[ACGT]ACC
BstPI 1110 GGTGACC G^GT[ACGT]ACC
BstUI 1932 CGCG CG^CG
CelII 1161 GCTCAGC GC^T[ACGT]AGC
DraIII 2153 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 943 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1110 GGTGACC G^GT[ACGT]ACC
EcoRI 408 GAATTC G^AATTC
Hin1I 1612 GACGTC G[GA]^CG[CT]C
MvnI 1932 CGCG CG^CG
NheI 1923 GCTAGC G^CTAGC
NspI 1034 ACATGT [GA]CATG^[CT]
NspV 2021 TTCGAA TT^CGAA
PmaCI 2153 CACGTG CAC^GTG
PmlI 2153 CACGTG CAC^GTG
PpuMI 633 AGGACCT [GA]G^G[AT]CC[CT]
SfuI 2021 TTCGAA TT^CGAA
SspI 1022 AATATT AAT^ATT
StuI 1286 AGGCCT AGG^CCT
TfiI 762 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 24 enzymes) #
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ApaI 1451 GGGCCC GGGCC^C
2107 GGGCCC
Asp700I 438 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1320 GAAATTATTC
BalI 131 TGGCCA TGG^CCA
603 TGGCCA
BanI 416 GGCACC G^G[CT][GA]CC
1964 GGCACC
BbvCI 1998 CCTCAGC CCTCAGC(-5/-2)
2112 CCTCAGC
BcgI 1505 CGAGCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1244 GCACGTTCTTCG
BcnI 255 CCGGG CC^[GC]GG
1338 CCGGG
BspMI 49 GCAGGT ACCTGC(4/8)
1425 GCAGGT
BssSI 1741 CACGAG CACGAG(-5/-1)
1003 CTCGTG
BstXI 45 CCAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
207 CCAGAACTGTGG
EaeI 131 TGGCCA [CT]^GGCC[GA]
603 TGGCCA
EcoNI 320 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2129 CCTTTGCCAGG
HaeII 1717 GGCGCT [GA]GCGC^[CT]
1835 AGCGCC
MluNI 131 TGGCCA TGG^CCA
603 TGGCCA
MscI 131 TGGCCA TGG^CCA
603 TGGCCA
MseI 562 TTAA T^TAA
569 TTAA
NciI 255 CCGGG CC^[GC]GG
1338 CCGGG
NcoI 121 CCATGG C^CATGG
1968 CCATGG
PvuII 766 CAGCTG CAG^CTG
1658 CAGCTG
SapI 25 GAAGAGC GCTCTTC(1/4)
891 GCTCTTC
SmlI 700 CTCAAG C^T[CT][GA]AG
919 CTCAAG
Tru9I 562 TTAA T^TAA
569 TTAA
TspRI 128 CAGTG CA[GC]TG[ACGT][ACGT]^
1329 CACTG
XmnI 438 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1320 GAAATTATTC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanII BbvI BfaI BglII
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TthHB8I Van91I XhoII