################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbrPI BbsI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EarI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MamI MboII MflI MluI MluNI MroI MscI MseI NaeI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AlwI 645 GGATC GGATC(4/5) AlwNI 371 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 349 CCTAAGG CC^T[ACGT]AGG ApaI 480 GGGCCC GGGCC^C AvaII 628 GGTCC G^G[AT]CC BbvCI 178 GCTGAGG CCTCAGC(-5/-2) BfaI 40 CTAG C^TAG BglI 62 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 556 TACGTG [CT]AC^GT[GA] BsaWI 587 ACCGGA [AT]^CCGG[AT] BsgI 106 GTGCAG GTGCAG(16/14) BsiEI 297 CGGCCG CG[GA][CT]^CG BsrBI 603 CCGCTC CCGCTC(-3/-3) BsrDI 683 GCAATG GCAATG(2/0) BsrI 706 ACTGG ACTGG(1/-1) BssHII 323 GCGCGC G^CGCGC Bsu36I 349 CCTAAGG CC^T[ACGT]AGG DpnII 646 GATC ^GATC DraII 627 AGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 297 CGGCCG [CT]^GGCC[GA] EagI 297 CGGCCG C^GGCCG EclXI 297 CGGCCG C^GGCCG Eco52I 297 CGGCCG C^GGCCG Eco81I 349 CCTAAGG CC^T[ACGT]AGG EcoO109I 627 AGGTCCC [GA]G^G[ACGT]CC[CT] MaeI 40 CTAG C^TAG MaeII 557 ACGT A^CGT MaeIII 569 GTGAC ^GT[ACGT]AC MboI 646 GATC ^GATC MfeI 564 CAATTG C^AATTG MslI 471 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 564 CAATTG C^AATTG NdeII 646 GATC ^GATC NheI 39 GCTAGC G^CTAGC NlaIII 485 CATG CATG^ PleI 502 GACTC GAGTC(4/5) PpuMI 627 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 525 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 646 GATC ^GATC TaqI 576 TCGA T^CGA Tsp45I 569 GTGAC ^GT[GC]AC TthHB8I 576 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 70 GGCGCC G[GA]^CG[CT]C 273 GGCGCC BanII 480 GGGCCC G[GA]GC[CT]^C 542 GGGCTC BbeI 70 GGCGCC GGCGC^C 273 GGCGCC BcgI 301 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 625 CGAGGTCCCTGC BpmI 520 CTCCAG CTGGAG(16/14) 674 CTCCAG BsaHI 70 GGCGCC G[GA]^CG[CT]C 273 GGCGCC BsaI 404 GAGACC GGTCTC(1/5) 671 GGTCTC BsmFI 629 GTCCC GGGAC(10/14) 660 GTCCC BstAPI 99 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 370 GCAGGCACTGC EcoT14I 74 CCTTGG C^C[AT][AT]GG 260 CCAAGG HapII 316 CCGG C^CGG 588 CCGG Hin1I 70 GGCGCC G[GA]^CG[CT]C 273 GGCGCC HpaII 316 CCGG C^CGG 588 CCGG KasI 70 GGCGCC G^GCGCC 273 GGCGCC MspA1I 25 CCGCTG C[AC]G^C[GT]G 306 CCGCTG MspI 316 CCGG C^CGG 588 CCGG NarI 70 GGCGCC GG^CGCC 273 GGCGCC PstI 244 CTGCAG CTGCA^G 511 CTGCAG SfcI 244 CTGCAG C^T[GA][CT]AG 511 CTGCAG SfoI 70 GGCGCC GGC^GCC 273 GGCGCC StyI 74 CCTTGG C^C[AT][AT]GG 260 CCAAGG TfiI 345 GAATC G^A[AT]TC 417 GAATC Tsp509I 451 AATT ^AATT 565 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccI AccII AciI AluI BanI BbvI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI ItaI MnlI MvaI MvnI MwoI NlaIV Sau96I ScrFI TseI TspRI