Restriction map for
pF1KB6962
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbrPI BbsI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BseAI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KpnI Ksp632I KspI MamI
MboII MflI MluI MluNI MroI
MscI MseI NaeI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AlwI 645 GGATC GGATC(4/5)
AlwNI 371 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 349 CCTAAGG CC^T[ACGT]AGG
ApaI 480 GGGCCC GGGCC^C
AvaII 628 GGTCC G^G[AT]CC
BbvCI 178 GCTGAGG CCTCAGC(-5/-2)
BfaI 40 CTAG C^TAG
BglI 62 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 556 TACGTG [CT]AC^GT[GA]
BsaWI 587 ACCGGA [AT]^CCGG[AT]
BsgI 106 GTGCAG GTGCAG(16/14)
BsiEI 297 CGGCCG CG[GA][CT]^CG
BsrBI 603 CCGCTC CCGCTC(-3/-3)
BsrDI 683 GCAATG GCAATG(2/0)
BsrI 706 ACTGG ACTGG(1/-1)
BssHII 323 GCGCGC G^CGCGC
Bsu36I 349 CCTAAGG CC^T[ACGT]AGG
DpnII 646 GATC ^GATC
DraII 627 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 297 CGGCCG [CT]^GGCC[GA]
EagI 297 CGGCCG C^GGCCG
EclXI 297 CGGCCG C^GGCCG
Eco52I 297 CGGCCG C^GGCCG
Eco81I 349 CCTAAGG CC^T[ACGT]AGG
EcoO109I 627 AGGTCCC [GA]G^G[ACGT]CC[CT]
MaeI 40 CTAG C^TAG
MaeII 557 ACGT A^CGT
MaeIII 569 GTGAC ^GT[ACGT]AC
MboI 646 GATC ^GATC
MfeI 564 CAATTG C^AATTG
MslI 471 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 564 CAATTG C^AATTG
NdeII 646 GATC ^GATC
NheI 39 GCTAGC G^CTAGC
NlaIII 485 CATG CATG^
PleI 502 GACTC GAGTC(4/5)
PpuMI 627 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 525 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 646 GATC ^GATC
TaqI 576 TCGA T^CGA
Tsp45I 569 GTGAC ^GT[GC]AC
TthHB8I 576 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 70 GGCGCC G[GA]^CG[CT]C
273 GGCGCC
BanII 480 GGGCCC G[GA]GC[CT]^C
542 GGGCTC
BbeI 70 GGCGCC GGCGC^C
273 GGCGCC
BcgI 301 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
625 CGAGGTCCCTGC
BpmI 520 CTCCAG CTGGAG(16/14)
674 CTCCAG
BsaHI 70 GGCGCC G[GA]^CG[CT]C
273 GGCGCC
BsaI 404 GAGACC GGTCTC(1/5)
671 GGTCTC
BsmFI 629 GTCCC GGGAC(10/14)
660 GTCCC
BstAPI 99 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
370 GCAGGCACTGC
EcoT14I 74 CCTTGG C^C[AT][AT]GG
260 CCAAGG
HapII 316 CCGG C^CGG
588 CCGG
Hin1I 70 GGCGCC G[GA]^CG[CT]C
273 GGCGCC
HpaII 316 CCGG C^CGG
588 CCGG
KasI 70 GGCGCC G^GCGCC
273 GGCGCC
MspA1I 25 CCGCTG C[AC]G^C[GT]G
306 CCGCTG
MspI 316 CCGG C^CGG
588 CCGG
NarI 70 GGCGCC GG^CGCC
273 GGCGCC
PstI 244 CTGCAG CTGCA^G
511 CTGCAG
SfcI 244 CTGCAG C^T[GA][CT]AG
511 CTGCAG
SfoI 70 GGCGCC GGC^GCC
273 GGCGCC
StyI 74 CCTTGG C^C[AT][AT]GG
260 CCAAGG
TfiI 345 GAATC G^A[AT]TC
417 GAATC
Tsp509I 451 AATT ^AATT
565 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccI AccII AciI AluI BanI
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
ItaI MnlI MvaI MvnI MwoI
NlaIV Sau96I ScrFI TseI TspRI