Restriction map for pF1KB6962
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BciVI       
BclI        BcnI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BseAI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrFI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        Eam1105I    
EarI        Eco47III    EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
HphI        KpnI        Ksp632I     KspI        MamI        
MboII       MflI        MluI        MluNI       MroI        
MscI        MseI        NaeI        NciI        NcoI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AlwI          645  GGATC                GGATC(4/5)
AlwNI         371  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          349  CCTAAGG              CC^T[ACGT]AGG
ApaI          480  GGGCCC               GGGCC^C
AvaII         628  GGTCC                G^G[AT]CC
BbvCI         178  GCTGAGG              CCTCAGC(-5/-2)
BfaI           40  CTAG                 C^TAG
BglI           62  GCCCAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         556  TACGTG               [CT]AC^GT[GA]
BsaWI         587  ACCGGA               [AT]^CCGG[AT]
BsgI          106  GTGCAG               GTGCAG(16/14)
BsiEI         297  CGGCCG               CG[GA][CT]^CG
BsrBI         603  CCGCTC               CCGCTC(-3/-3)
BsrDI         683  GCAATG               GCAATG(2/0)
BsrI          706  ACTGG                ACTGG(1/-1)
BssHII        323  GCGCGC               G^CGCGC
Bsu36I        349  CCTAAGG              CC^T[ACGT]AGG
DpnII         646  GATC                 ^GATC
DraII         627  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI          297  CGGCCG               [CT]^GGCC[GA]
EagI          297  CGGCCG               C^GGCCG
EclXI         297  CGGCCG               C^GGCCG
Eco52I        297  CGGCCG               C^GGCCG
Eco81I        349  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      627  AGGTCCC              [GA]G^G[ACGT]CC[CT]
MaeI           40  CTAG                 C^TAG
MaeII         557  ACGT                 A^CGT
MaeIII        569  GTGAC                ^GT[ACGT]AC
MboI          646  GATC                 ^GATC
MfeI          564  CAATTG               C^AATTG
MslI          471  CACTGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          564  CAATTG               C^AATTG
NdeII         646  GATC                 ^GATC
NheI           39  GCTAGC               G^CTAGC
NlaIII        485  CATG                 CATG^
PleI          502  GACTC                GAGTC(4/5)
PpuMI         627  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         525  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI        646  GATC                 ^GATC
TaqI          576  TCGA                 T^CGA
Tsp45I        569  GTGAC                ^GT[GC]AC
TthHB8I       576  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI           70  GGCGCC               G[GA]^CG[CT]C
              273  GGCGCC              
BanII         480  GGGCCC               G[GA]GC[CT]^C
              542  GGGCTC              
BbeI           70  GGCGCC               GGCGC^C
              273  GGCGCC              
BcgI          301  CGAGGCCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              625  CGAGGTCCCTGC        
BpmI          520  CTCCAG               CTGGAG(16/14)
              674  CTCCAG              
BsaHI          70  GGCGCC               G[GA]^CG[CT]C
              273  GGCGCC              
BsaI          404  GAGACC               GGTCTC(1/5)
              671  GGTCTC              
BsmFI         629  GTCCC                GGGAC(10/14)
              660  GTCCC               
BstAPI         99  GCAAGGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              370  GCAGGCACTGC         
EcoT14I        74  CCTTGG               C^C[AT][AT]GG
              260  CCAAGG              
HapII         316  CCGG                 C^CGG
              588  CCGG                
Hin1I          70  GGCGCC               G[GA]^CG[CT]C
              273  GGCGCC              
HpaII         316  CCGG                 C^CGG
              588  CCGG                
KasI           70  GGCGCC               G^GCGCC
              273  GGCGCC              
MspA1I         25  CCGCTG               C[AC]G^C[GT]G
              306  CCGCTG              
MspI          316  CCGG                 C^CGG
              588  CCGG                
NarI           70  GGCGCC               GG^CGCC
              273  GGCGCC              
PstI          244  CTGCAG               CTGCA^G
              511  CTGCAG              
SfcI          244  CTGCAG               C^T[GA][CT]AG
              511  CTGCAG              
SfoI           70  GGCGCC               GGC^GCC
              273  GGCGCC              
StyI           74  CCTTGG               C^C[AT][AT]GG
              260  CCAAGG              
TfiI          345  GAATC                G^A[AT]TC
              417  GAATC               
Tsp509I       451  AATT                 ^AATT
              565  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccI        AccII       AciI        AluI        BanI        
BbvI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HhaI        HinP1I      HinfI       
ItaI        MnlI        MvaI        MvnI        MwoI        
NlaIV       Sau96I      ScrFI       TseI        TspRI       
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