Restriction map for
pF1KB4675
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BclI BcnI BglI BglII
BlnI BmyI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
BspEI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NciI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Van91I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AciI 4 CCGC CCGC(-3/-1)
AclI 909 AACGTT AA^CGTT
AflII 1030 CTTAAG C^TTAAG
AlwNI 773 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1357 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 412 GGTGCC G^G[CT][GA]CC
BbvCI 245 CCTCAGC CCTCAGC(-5/-2)
BfrI 1030 CTTAAG C^TTAAG
BpmI 110 CTGGAG CTGGAG(16/14)
BseRI 681 CTCCTC GAGGAG(10/8)
BsiYI 395 CCCTTAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 395 CCCTTAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1181 GTCCC GGGAC(10/14)
BspDI 727 ATCGAT AT^CGAT
BspMI 409 GCAGGT ACCTGC(4/8)
BsrBI 4 CCGCTC CCGCTC(-3/-3)
BsrFI 953 GCCGGC [GA]^CCGG[CT]
BssKI 1278 CCAGG ^CC[ACGT]GG
BstAPI 528 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 1278 CCAGG CC^[AT]GG
BstXI 974 CCATTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1125 GGATCT [GA]^GATC[CT]
CfoI 445 GCGC GCG^C
Cfr10I 953 GCCGGC [GA]^CCGG[CT]
Cfr13I 1441 GGGCC G^G[ACGT]CC
ClaI 727 ATCGAT AT^CGAT
DsaI 485 CCATGG C^C[GA][CT]GG
EcoRII 1278 CCAGG ^CC[AT]GG
EcoRV 1384 GATATC GAT^ATC
HaeIII 1442 GGCC GG^CC
HapII 954 CCGG C^CGG
HhaI 445 GCGC GCG^C
HinP1I 445 GCGC G^CGC
HincII 105 GTTGAC GT[CT]^[GA]AC
HindII 105 GTTGAC GT[CT]^[GA]AC
HpaII 954 CCGG C^CGG
MflI 1125 GGATCT [GA]^GATC[CT]
MslI 975 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 94 CAGCTG C[AC]G^C[GT]G
MspI 954 CCGG C^CGG
MvaI 1278 CCAGG CC^[AT]GG
NaeI 953 GCCGGC GCC^GGC
NcoI 485 CCATGG C^CATGG
NgoMIV 953 GCCGGC G^CCGGC
PflFI 1357 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 108 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 909 AACGTT AA^CGTT
PvuII 94 CAGCTG CAG^CTG
SapI 229 GCTCTTC GCTCTTC(1/4)
Sau96I 1441 GGGCC G^G[ACGT]CC
ScaI 1266 AGTACT AGT^ACT
ScrFI 1278 CCAGG CC^[ACGT]GG
TspRI 361 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1357 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1128 TCTAGA T^CTAGA
XhoII 1125 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 1112 GATCC GGATC(4/5)
1125 GGATC
BbsI 268 GAAGAC GAAGAC(2/6)
259 GTCTTC
BciVI 611 GGATAC GTATCC(6/5)
1246 GGATAC
BfaI 1129 CTAG C^TAG
1289 CTAG
BlpI 91 GCTCAGC GC^T[ACGT]AGC
822 GCTTAGC
Bpu1102I 91 GCTCAGC GC^T[ACGT]AGC
822 GCTTAGC
BpuAI 268 GAAGAC GAAGAC(2/6)
259 GTCTTC
BsmI 348 GAATGC GAATGC(1/-1)
1017 GAATGC
Bsp1407I 769 TGTACA T^GTACA
1075 TGTACA
BsrGI 769 TGTACA T^GTACA
1075 TGTACA
CelII 91 GCTCAGC GC^T[ACGT]AGC
822 GCTTAGC
EarI 230 CTCTTC CTCTTC(1/4)
1465 GAAGAG
EcoT14I 327 CCTTGG C^C[AT][AT]GG
485 CCATGG
Ksp632I 230 CTCTTC CTCTTC(1/4)
1465 GAAGAG
MaeI 1129 CTAG C^TAG
1289 CTAG
MaeII 910 ACGT A^CGT
1427 ACGT
NlaIV 412 GGTGCC GG[ACGT]^[ACGT]CC
1231 GGCTCC
PstI 458 CTGCAG CTGCA^G
526 CTGCAG
SfcI 458 CTGCAG C^T[GA][CT]AG
526 CTGCAG
SmlI 417 CTTGAG C^T[CT][GA]AG
1030 CTTAAG
SspBI 769 TGTACA T^GTACA
1075 TGTACA
StyI 327 CCTTGG C^C[AT][AT]GG
485 CCATGG
Tsp45I 793 GTCAC ^GT[GC]AC
1166 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsaJI BsmAI BsrI BstF5I Cac8I
DdeI DpnII Fnu4HI FokI HinfI
HphI ItaI MaeIII MboI MboII
MnlI MseI MwoI NdeII NlaIII
PleI RsaI Sau3AI SfaNI TaqI
TfiI Tru9I TseI Tsp509I TthHB8I