Restriction map for
pF1KE2427
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbvCI BclI BfrI
BlnI BlpI Bpu1102I BsgI BsiEI
BsiWI BsmI BspDI BspMI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1774 GTCTAC GT^[AC][GT]AC
AccIII 1682 TCCGGA T^CCGGA
AgeI 1067 ACCGGT A^CCGGT
Asp700I 1551 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 555 GGACC G^G[AT]CC
BamHI 1679 GGATCC G^GATCC
BbeI 3 GGCGCC GGCGC^C
BbrPI 802 CACGTG CAC^GTG
BcgI 1211 GCACATTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1389 GGATAC GTATCC(6/5)
BsaAI 802 CACGTG [CT]AC^GT[GA]
BsaBI 1150 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 319 GAGACC GGTCTC(1/5)
BseAI 1682 TCCGGA T^CCGGA
BsmBI 451 CGTCTC CGTCTC(1/5)
Bsp1407I 1373 TGTACA T^GTACA
BspEI 1682 TCCGGA T^CCGGA
BspLU11I 1793 ACATGT A^CATGT
BsrFI 1067 ACCGGT [GA]^CCGG[CT]
BsrGI 1373 TGTACA T^GTACA
BstAPI 430 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 4 GCGC GCG^C
Cfr10I 1067 ACCGGT [GA]^CCGG[CT]
DraIII 431 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
HaeII 3 GGCGCC [GA]GCGC^[CT]
HgaI 69 GACGC GACGC(5/10)
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
KasI 3 GGCGCC G^GCGCC
MamI 1150 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1682 TCCGGA T^CCGGA
MslI 1614 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 3 GGCGCC GG^CGCC
PinAI 1067 ACCGGT A^CCGGT
PmaCI 802 CACGTG CAC^GTG
PmlI 802 CACGTG CAC^GTG
PpuMI 554 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1299 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1090 GAGCTC GAGCT^C
SapI 1665 GCTCTTC GCTCTTC(1/4)
SfiI 276 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3 GGCGCC GGC^GCC
SmaI 1181 CCCGGG CCC^GGG
SspBI 1373 TGTACA T^GTACA
XcmI 89 CCAAGCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1181 CCCGGG C^CCGGG
XmaI 1181 CCCGGG C^CCGGG
XmnI 1551 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 714 CGCG CG^CG
1591 CGCG
AcyI 3 GGCGCC G[GA]^CG[CT]C
69 GACGCC
AflIII 1370 ACGTGT A^C[GA][CT]GT
1793 ACATGT
AvaI 580 CTCGGG C^[CT]CG[GA]G
1181 CCCGGG
BalI 1329 TGGCCA TGG^CCA
1817 TGGCCA
BfaI 919 CTAG C^TAG
1721 CTAG
BglI 277 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1864 GCCCAGCAGGC
BpmI 595 CTCCAG CTGGAG(16/14)
958 CTCCAG
BsaHI 3 GGCGCC G[GA]^CG[CT]C
69 GACGCC
BsaWI 1067 ACCGGT [AT]^CCGG[AT]
1682 TCCGGA
BsoBI 580 CTCGGG C^[CT]CG[GA]G
1181 CCCGGG
BspHI 846 TCATGA T^CATGA
1007 TCATGA
BsrDI 1164 CATTGC GCAATG(2/0)
245 GCAATG
BstUI 714 CGCG CG^CG
1591 CGCG
DrdI 1039 GACTTCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1536 GACTGTCTTGTC
DsaI 1145 CCGTGG C^C[GA][CT]GG
1616 CCGTGG
EaeI 1329 TGGCCA [CT]^GGCC[GA]
1817 TGGCCA
Hin1I 3 GGCGCC G[GA]^CG[CT]C
69 GACGCC
MaeI 919 CTAG C^TAG
1721 CTAG
MluNI 1329 TGGCCA TGG^CCA
1817 TGGCCA
MscI 1329 TGGCCA TGG^CCA
1817 TGGCCA
MvnI 714 CGCG CG^CG
1591 CGCG
PflMI 53 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1332 CCAGGAAGTGG
PstI 493 CTGCAG CTGCA^G
770 CTGCAG
RcaI 846 TCATGA T^CATGA
1007 TCATGA
TaqI 1049 TCGA T^CGA
1534 TCGA
TfiI 145 GAATC G^A[AT]TC
253 GATTC
TthHB8I 1049 TCGA T^CGA
1534 TCGA
Van91I 53 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1332 CCAGGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI BanI
BanII BbsI BbvI BcnI BglII
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII