Restriction map for
pF1KE0099
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BpuAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HapII HincII HindII
HindIII HinfI HpaI HpaII KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MslI MspI MunI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 628 GGCGCC G[GA]^CG[CT]C
AhdI 154 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 466 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 315 CCTGAGG CC^T[ACGT]AGG
AspEI 154 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 548 TGCGCA TGC^GCA
BalI 113 TGGCCA TGG^CCA
BanI 628 GGCGCC G^G[CT][GA]CC
BbeI 628 GGCGCC GGCGC^C
BsaAI 124 TACGTG [CT]AC^GT[GA]
BsaHI 628 GGCGCC G[GA]^CG[CT]C
BsiEI 519 CGACCG CG[GA][CT]^CG
BssSI 475 CACGAG CACGAG(-5/-1)
BstAPI 474 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 129 GGATG GGATG(2/0)
BstYI 336 GGATCT [GA]^GATC[CT]
Bsu36I 315 CCTGAGG CC^T[ACGT]AGG
Eam1105I 154 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 31 CTCTTC CTCTTC(1/4)
Eco81I 315 CCTGAGG CC^T[ACGT]AGG
FauI 697 CCCGC CCCGC(4/6)
FokI 129 GGATG GGATG(9/13)
FspI 548 TGCGCA TGC^GCA
HgaI 541 GACGC GACGC(5/10)
Hin1I 628 GGCGCC G[GA]^CG[CT]C
HphI 348 GGTGA GGTGA(8/7)
KasI 628 GGCGCC G^GCGCC
Ksp632I 31 CTCTTC CTCTTC(1/4)
MaeII 125 ACGT A^CGT
MflI 336 GGATCT [GA]^GATC[CT]
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
NarI 628 GGCGCC GG^CGCC
NlaIII 535 CATG CATG^
SapI 30 GCTCTTC GCTCTTC(1/4)
SfcI 567 CTACAG C^T[GA][CT]AG
SfoI 628 GGCGCC GGC^GCC
StuI 720 AGGCCT AGG^CCT
XcmI 116 CCACTGTGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 336 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 96 GGATC GGATC(4/5)
336 GGATC
Aor51HI 587 AGCGCT AGC^GCT
763 AGCGCT
AvaI 703 CTCGAG C^[CT]CG[GA]G
769 CTCGAG
AvaII 108 GGACC G^G[AT]CC
708 GGACC
BglI 497 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
631 GCCAGACTGGC
BlpI 492 GCTGAGC GC^T[ACGT]AGC
678 GCTCAGC
BpmI 340 CTGGAG CTGGAG(16/14)
736 CTGGAG
Bpu1102I 492 GCTGAGC GC^T[ACGT]AGC
678 GCTCAGC
BsgI 472 CTGCAC GTGCAG(16/14)
361 GTGCAG
BsmAI 152 GAGAC GTCTC(1/5)
304 GAGAC
BsmFI 221 GGGAC GGGAC(10/14)
162 GTCCC
BsoBI 703 CTCGAG C^[CT]CG[GA]G
769 CTCGAG
CelII 492 GCTGAGC GC^T[ACGT]AGC
678 GCTCAGC
DpnII 97 GATC ^GATC
337 GATC
Eco47III 587 AGCGCT AGC^GCT
763 AGCGCT
EcoNI 294 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
315 CCTGAGGCAGG
MaeIII 229 GTGAC ^GT[ACGT]AC
271 GTGAC
MboI 97 GATC ^GATC
337 GATC
MboII 387 GAAGA GAAGA(8/7)
32 TCTTC
MspA1I 144 CAGCGG C[AC]G^C[GT]G
433 CCGCTG
NdeII 97 GATC ^GATC
337 GATC
PaeR7I 703 CTCGAG C^TCGAG
769 CTCGAG
PflMI 420 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
585 CCAGCGCTTGG
PpuMI 107 AGGACCT [GA]G^G[AT]CC[CT]
707 AGGACCT
SacI 409 GAGCTC GAGCT^C
445 GAGCTC
Sau3AI 97 GATC ^GATC
337 GATC
Tsp45I 229 GTGAC ^GT[GC]AC
271 GTGAC
TspRI 117 CACTG CA[GC]TG[ACGT][ACGT]^
500 CACTG
Van91I 420 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
585 CCAGCGCTTGG
XhoI 703 CTCGAG C^TCGAG
769 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BanII BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI HaeII
HaeIII HhaI HinP1I ItaI MnlI
MvaI MvnI MwoI NlaIV RsaI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TseI TthHB8I