Restriction map for
pF1KB6184
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 14 CGCG CG^CG
AccIII 1745 TCCGGA T^CCGGA
ApaI 128 GGGCCC GGGCC^C
AspI 238 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1092 GGTGCC G^G[CT][GA]CC
BbvCI 123 GCTGAGG CCTCAGC(-5/-2)
BcgI 1184 GCACTGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1115 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 777 AGATCT A^GATCT
BsaBI 1167 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1745 TCCGGA [AT]^CCGG[AT]
BseAI 1745 TCCGGA T^CCGGA
BspEI 1745 TCCGGA T^CCGGA
BspMI 1965 ACCTGC ACCTGC(4/8)
BsrDI 201 GCAATG GCAATG(2/0)
BsrFI 344 GCCGGC [GA]^CCGG[CT]
BstEII 1452 GGTCACC G^GT[ACGT]ACC
BstPI 1452 GGTCACC G^GT[ACGT]ACC
BstUI 14 CGCG CG^CG
CfoI 15 GCGC GCG^C
Cfr10I 344 GCCGGC [GA]^CCGG[CT]
DraIII 448 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 271 GACCAAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1452 GGTCACC G^GT[ACGT]ACC
EcoRI 814 GAATTC G^AATTC
HhaI 15 GCGC GCG^C
HinP1I 15 GCGC G^CGC
MamI 1167 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1745 TCCGGA T^CCGGA
MslI 1425 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 14 CGCG CG^CG
NaeI 344 GCCGGC GCC^GGC
NgoMIV 344 GCCGGC G^CCGGC
NspI 1497 ACATGC [GA]CATG^[CT]
PaeR7I 616 CTCGAG C^TCGAG
PflFI 238 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1004 CTGCAG CTGCA^G
SacI 613 GAGCTC GAGCT^C
ScaI 1526 AGTACT AGT^ACT
SfcI 1004 CTGCAG C^T[GA][CT]AG
Tth111I 238 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 616 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 814 GAATTC [GA]^AATT[CT]
979 AAATTT
AlwI 1167 GATCC GGATC(4/5)
24 GGATC
AocI 391 CCTGAGG CC^T[ACGT]AGG
991 CCTGAGG
ApoI 814 GAATTC [GA]^AATT[CT]
979 AAATTT
BalI 170 TGGCCA TGG^CCA
1122 TGGCCA
BfaI 917 CTAG C^TAG
1030 CTAG
BstAPI 431 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1059 GCAAGTCCTGC
BstYI 24 GGATCT [GA]^GATC[CT]
777 AGATCT
Bsu36I 391 CCTGAGG CC^T[ACGT]AGG
991 CCTGAGG
DraII 127 AGGGCCC [GA]G^G[ACGT]CC[CT]
128 GGGCCCT
EaeI 170 TGGCCA [CT]^GGCC[GA]
1122 TGGCCA
Eco81I 391 CCTGAGG CC^T[ACGT]AGG
991 CCTGAGG
EcoO109I 127 AGGGCCC [GA]G^G[ACGT]CC[CT]
128 GGGCCCT
MaeI 917 CTAG C^TAG
1030 CTAG
MflI 24 GGATCT [GA]^GATC[CT]
777 AGATCT
MluNI 170 TGGCCA TGG^CCA
1122 TGGCCA
MscI 170 TGGCCA TGG^CCA
1122 TGGCCA
MseI 293 TTAA T^TAA
1337 TTAA
NcoI 253 CCATGG C^CATGG
1532 CCATGG
PflMI 1440 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1979 CCAGTGCCTGG
PleI 574 GAGTC GAGTC(4/5)
946 GAGTC
PvuII 121 CAGCTG CAG^CTG
1036 CAGCTG
Tru9I 293 TTAA T^TAA
1337 TTAA
Tsp509I 815 AATT ^AATT
980 AATT
Van91I 1440 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1979 CCAGTGCCTGG
XhoII 24 GGATCT [GA]^GATC[CT]
777 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I