Restriction map for
pF1KB6665
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 1267 GGCGCC G[GA]^CG[CT]C
AlwI 837 GGATC GGATC(4/5)
AocI 32 CCTTAGG CC^T[ACGT]AGG
AspI 112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1023 CCCGGG C^[CT]CG[GA]G
AviII 554 TGCGCA TGC^GCA
BbeI 1267 GGCGCC GGCGC^C
BsaAI 1252 TACGTG [CT]AC^GT[GA]
BsaHI 1267 GGCGCC G[GA]^CG[CT]C
BsaI 685 GAGACC GGTCTC(1/5)
BsgI 280 CTGCAC GTGCAG(16/14)
BsiEI 392 CGGCCG CG[GA][CT]^CG
BsmBI 537 CGTCTC CGTCTC(1/5)
BsoBI 1023 CCCGGG C^[CT]CG[GA]G
BspMI 688 ACCTGC ACCTGC(4/8)
BstEII 966 GGTTACC G^GT[ACGT]ACC
BstPI 966 GGTTACC G^GT[ACGT]ACC
BstXI 1247 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 837 GGATCT [GA]^GATC[CT]
Bsu36I 32 CCTTAGG CC^T[ACGT]AGG
DpnII 838 GATC ^GATC
DrdI 529 GACAGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 392 CGGCCG C^GGCCG
EclXI 392 CGGCCG C^GGCCG
Eco52I 392 CGGCCG C^GGCCG
Eco81I 32 CCTTAGG CC^T[ACGT]AGG
EcoO65I 966 GGTTACC G^GT[ACGT]ACC
FspI 554 TGCGCA TGC^GCA
Hin1I 1267 GGCGCC G[GA]^CG[CT]C
HinfI 1071 GAGTC G^A[ACGT]TC
KasI 1267 GGCGCC G^GCGCC
MaeII 1253 ACGT A^CGT
MboI 838 GATC ^GATC
MflI 837 GGATCT [GA]^GATC[CT]
MslI 873 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1267 GGCGCC GG^CGCC
NdeII 838 GATC ^GATC
NspI 243 GCATGC [GA]CATG^[CT]
PflFI 112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1071 GAGTC GAGTC(4/5)
PstI 562 CTGCAG CTGCA^G
Sau3AI 838 GATC ^GATC
SexAI 113 ACCTGGT A^CC[AT]GGT
SfiI 1225 GGCCGCATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1267 GGCGCC GGC^GCC
SmaI 1023 CCCGGG CCC^GGG
SphI 243 GCATGC GCATG^C
Tth111I 112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1212 CCAGTTGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 837 GGATCT [GA]^GATC[CT]
XmaCI 1023 CCCGGG C^CCGGG
XmaI 1023 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 76 GTAC GT^AC
1239 GTAC
ApaI 179 GGGCCC GGGCC^C
210 GGGCCC
BbsI 492 GAAGAC GAAGAC(2/6)
600 GAAGAC
BglI 586 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1226 GCCGCATTGGC
BpuAI 492 GAAGAC GAAGAC(2/6)
600 GAAGAC
BsmAI 685 GAGAC GTCTC(1/5)
538 GTCTC
BsrBI 265 GAGCGG CCGCTC(-3/-3)
322 GAGCGG
BsrFI 98 ACCGGC [GA]^CCGG[CT]
356 ACCGGC
BstF5I 486 GGATG GGATG(2/0)
1047 GGATG
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
356 ACCGGC
DsaI 200 CCGTGG C^C[GA][CT]GG
861 CCACGG
FokI 486 GGATG GGATG(9/13)
1047 GGATG
HgaI 16 GACGC GACGC(5/10)
624 GACGC
HphI 1101 GGTGA GGTGA(8/7)
740 TCACC
PpuMI 110 GGGACCT [GA]G^G[AT]CC[CT]
1027 GGGTCCC
PvuII 120 CAGCTG CAG^CTG
550 CAGCTG
RsaI 76 GTAC GT^AC
1239 GTAC
SfaNI 143 GCATC GCATC(5/9)
639 GCATC
SfcI 63 CTACAG C^T[GA][CT]AG
562 CTGCAG
TaqI 146 TCGA T^CGA
896 TCGA
Tsp45I 797 GTGAC ^GT[GC]AC
827 GTGAC
TthHB8I 146 TCGA T^CGA
896 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BalI BanI BanII BbvI BcnI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssHII BssKI BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
Ksp632I MaeIII MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PflMI
Sau96I ScrFI StuI StyI TseI
TspRI Van91I