Restriction map for
pF1KB5545
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BseAI BsgI BsiHKAI BsiWI BsmI
BsoBI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV EcoT14I FseI
FspI HaeII HgaI Hin1I KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SacI SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1535 GTCGAC GT^[AC][GT]AC
AlwNI 860 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1544 GGGCCC GGGCC^C
Asp700I 875 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 36 TGATCA T^GATCA
BcnI 794 CCCGG CC^[GC]GG
BglII 721 AGATCT A^GATCT
BpmI 989 CTGGAG CTGGAG(16/14)
BsaBI 539 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 165 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 1441 TCCGGT [AT]^CCGG[AT]
BsiEI 1791 CGATCG CG[GA][CT]^CG
BsmFI 604 GTCCC GGGAC(10/14)
Bsp1407I 1259 TGTACA T^GTACA
BspDI 1789 ATCGAT AT^CGAT
BsrBI 1111 GAGCGG CCGCTC(-3/-3)
BsrDI 1669 GCAATG GCAATG(2/0)
BsrGI 1259 TGTACA T^GTACA
BstEII 686 GGTCACC G^GT[ACGT]ACC
BstPI 686 GGTCACC G^GT[ACGT]ACC
BstYI 721 AGATCT [GA]^GATC[CT]
CfoI 54 GCGC GCG^C
ClaI 1789 ATCGAT AT^CGAT
EcoNI 610 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 686 GGTCACC G^GT[ACGT]ACC
EcoT22I 1694 ATGCAT ATGCA^T
FauI 1113 GCGGG CCCGC(4/6)
FbaI 36 TGATCA T^GATCA
HhaI 54 GCGC GCG^C
HinP1I 54 GCGC G^CGC
HindIII 89 AAGCTT A^AGCTT
MamI 539 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 721 AGATCT [GA]^GATC[CT]
MspA1I 908 CAGCTG C[AC]G^C[GT]G
NciI 794 CCCGG CC^[GC]GG
NsiI 1694 ATGCAT ATGCA^T
PflMI 388 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 602 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 44 CTGCAG CTGCA^G
PvuI 1791 CGATCG CGAT^CG
PvuII 908 CAGCTG CAG^CTG
SalI 1535 GTCGAC G^TCGAC
ScaI 175 AGTACT AGT^ACT
SmlI 884 CTCAAG C^T[CT][GA]AG
SspBI 1259 TGTACA T^GTACA
Van91I 388 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 721 AGATCT [GA]^GATC[CT]
XmnI 875 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 229 CCGC CCGC(-3/-1)
1113 GCGG
AlwI 78 GGATC GGATC(4/5)
931 GGATC
AvaII 69 GGACC G^G[AT]CC
603 GGTCC
BanII 642 GAGCCC G[GA]GC[CT]^C
1544 GGGCCC
BmyI 642 GAGCCC G[AGT]GC[ACT]^C
1544 GGGCCC
BsaI 942 GAGACC GGTCTC(1/5)
1037 GGTCTC
BsmBI 195 GAGACG CGTCTC(1/5)
772 GAGACG
Bsp1286I 642 GAGCCC G[AGT]GC[ACT]^C
1544 GGGCCC
BspMI 892 GCAGGT ACCTGC(4/8)
1117 GCAGGT
Cac8I 1018 GCCAGC GC[ACGT]^[ACGT]GC
1113 GCGGGC
DraIII 799 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1325 CACGGAGTG
EcoRI 1148 GAATTC G^AATTC
1809 GAATTC
HapII 795 CCGG C^CGG
1442 CCGG
HpaI 427 GTTAAC GTT^AAC
594 GTTAAC
HpaII 795 CCGG C^CGG
1442 CCGG
MaeII 483 ACGT A^CGT
1104 ACGT
MspI 795 CCGG C^CGG
1442 CCGG
XbaI 565 TCTAGA T^CTAGA
787 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI ApoI BanI
BbvI BfaI BseRI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I