##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        TseI        Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AciI           80  CCGC                 CCGC(-3/-1)
BciVI         157  GGATAC               GTATCC(6/5)
BclI          237  TGATCA               T^GATCA
BmyI           46  GGGCAC               G[AGT]GC[ACT]^C
BsaBI          87  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI         258  GTCTC                GTCTC(1/5)
BsmFI         245  GGGAC                GGGAC(10/14)
Bsp1286I       46  GGGCAC               G[AGT]GC[ACT]^C
BspMI         303  ACCTGC               ACCTGC(4/8)
BssKI         108  CCTGG                ^CC[ACGT]GG
BstEII         31  GGTTACC              G^GT[ACGT]ACC
BstF5I         93  CATCC                GGATG(2/0)
BstNI         108  CCTGG                CC^[AT]GG
BstPI          31  GGTTACC              G^GT[ACGT]ACC
DsaI           65  CCACGG               C^C[GA][CT]GG
EaeI           51  CGGCCA               [CT]^GGCC[GA]
EcoO65I        31  GGTTACC              G^GT[ACGT]ACC
EcoRII        108  CCTGG                ^CC[AT]GG
FbaI          237  TGATCA               T^GATCA
Fnu4HI         79  GCCGC                GC^[ACGT]GC
FokI           93  CATCC                GGATG(9/13)
HinfI         113  GAGTC                G^A[ACGT]TC
ItaI           79  GCCGC                GC^[ACGT]GC
MamI           87  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MnlI           76  GAGG                 CCTC(7/6)
MseI          208  TTAA                 T^TAA
MspA1I         80  CCGCTG               C[AC]G^C[GT]G
MvaI          108  CCTGG                CC^[AT]GG
NlaIII        187  CATG                 CATG^
NlaIV         288  GGTTCC               GG[ACGT]^[ACGT]CC
PleI          113  GAGTC                GAGTC(4/5)
PstI          323  CTGCAG               CTGCA^G
ScrFI         108  CCTGG                CC^[ACGT]GG
SfcI          323  CTGCAG               C^T[GA][CT]AG
Tru9I         208  TTAA                 T^TAA
Tsp45I        333  GTCAC                ^GT[GC]AC
TspRI         101  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwI          105  GATCC                GGATC(4/5)
               86  GGATC               
BbsI          146  GAAGAC               GAAGAC(2/6)
              182  GTCTTC              
BpuAI         146  GAAGAC               GAAGAC(2/6)
              182  GTCTTC              
BsaJI          65  CCACGG               C^C[ACGT][ACGT]GG
              108  CCTGGG              
EarI          260  CTCTTC               CTCTTC(1/4)
               73  GAAGAG              
HaeIII         52  GGCC                 GG^CC
               78  GGCC                
HphI          334  TCACC                GGTGA(8/7)
              349  TCACC               
Ksp632I       260  CTCTTC               CTCTTC(1/4)
               73  GAAGAG              
MaeIII         32  GTTAC                ^GT[ACGT]AC
              333  GTCAC               
MwoI           70  GCTGAAGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              298  GCTCCACCTGC         


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

BsiYI       BslI        DpnII       MboI        MboII       
NdeII       Sau3AI      SfaNI