################################## # NO CUT ENZYMES ( 211 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvaI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AflII 67 CTTAAG C^TTAAG AspHI 215 GTGCTC G[AT]GC[AT]^C BamHI 153 GGATCC G^GATCC BfrI 67 CTTAAG C^TTAAG BsaI 10 GAGACC GGTCTC(1/5) BseRI 220 CTCCTC GAGGAG(10/8) BsiHKAI 215 GTGCTC G[AT]GC[AT]^C BsiYI 189 CCCCTTCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 189 CCCCTTCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 10 GAGAC GTCTC(1/5) BstYI 153 GGATCC [GA]^GATC[CT] DpnII 154 GATC ^GATC EcoT14I 104 CCAAGG C^C[AT][AT]GG MboI 154 GATC ^GATC MflI 153 GGATCC [GA]^GATC[CT] MseI 68 TTAA T^TAA NdeII 154 GATC ^GATC NlaIV 153 GGATCC GG[ACGT]^[ACGT]CC Sau3AI 154 GATC ^GATC SfaNI 136 GATGC GCATC(5/9) SmlI 67 CTTAAG C^T[CT][GA]AG StyI 104 CCAAGG C^C[AT][AT]GG Tru9I 68 TTAA T^TAA XhoII 153 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AluI 65 AGCT AG^CT 82 AGCT AlwI 154 GATCC GGATC(4/5) 153 GGATC BbvI 109 GCAGC GCAGC(8/12) 112 GCAGC BcnI 15 CCCGG CC^[GC]GG 195 CCGGG BmyI 172 GTGCCC G[AGT]GC[ACT]^C 215 GTGCTC BsaJI 14 CCCCGG C^C[ACGT][ACGT]GG 104 CCAAGG Bsp1286I 172 GTGCCC G[AGT]GC[ACT]^C 215 GTGCTC BssKI 15 CCCGG ^CC[ACGT]GG 195 CCGGG Fnu4HI 109 GCAGC GC^[ACGT]GC 112 GCAGC HapII 16 CCGG C^CGG 195 CCGG HpaII 16 CCGG C^CGG 195 CCGG ItaI 109 GCAGC GC^[ACGT]GC 112 GCAGC MboII 201 GAAGA GAAGA(8/7) 209 TCTTC MslI 142 CACGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 165 CACCCCTGTG MspI 16 CCGG C^CGG 195 CCGG NciI 15 CCCGG CC^[GC]GG 195 CCGGG ScrFI 15 CCCGG CC^[ACGT]GG 195 CCGGG TseI 109 GCAGC G^C[AT]GC 112 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### Asp700I Cac8I HphI MnlI XmnI