##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HaeIII      HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AflII          67  CTTAAG               C^TTAAG
AspHI         215  GTGCTC               G[AT]GC[AT]^C
BamHI         153  GGATCC               G^GATCC
BfrI           67  CTTAAG               C^TTAAG
BsaI           10  GAGACC               GGTCTC(1/5)
BseRI         220  CTCCTC               GAGGAG(10/8)
BsiHKAI       215  GTGCTC               G[AT]GC[AT]^C
BsiYI         189  CCCCTTCCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          189  CCCCTTCCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          10  GAGAC                GTCTC(1/5)
BstYI         153  GGATCC               [GA]^GATC[CT]
DpnII         154  GATC                 ^GATC
EcoT14I       104  CCAAGG               C^C[AT][AT]GG
MboI          154  GATC                 ^GATC
MflI          153  GGATCC               [GA]^GATC[CT]
MseI           68  TTAA                 T^TAA
NdeII         154  GATC                 ^GATC
NlaIV         153  GGATCC               GG[ACGT]^[ACGT]CC
Sau3AI        154  GATC                 ^GATC
SfaNI         136  GATGC                GCATC(5/9)
SmlI           67  CTTAAG               C^T[CT][GA]AG
StyI          104  CCAAGG               C^C[AT][AT]GG
Tru9I          68  TTAA                 T^TAA
XhoII         153  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AluI           65  AGCT                 AG^CT
               82  AGCT                
AlwI          154  GATCC                GGATC(4/5)
              153  GGATC               
BbvI          109  GCAGC                GCAGC(8/12)
              112  GCAGC               
BcnI           15  CCCGG                CC^[GC]GG
              195  CCGGG               
BmyI          172  GTGCCC               G[AGT]GC[ACT]^C
              215  GTGCTC              
BsaJI          14  CCCCGG               C^C[ACGT][ACGT]GG
              104  CCAAGG              
Bsp1286I      172  GTGCCC               G[AGT]GC[ACT]^C
              215  GTGCTC              
BssKI          15  CCCGG                ^CC[ACGT]GG
              195  CCGGG               
Fnu4HI        109  GCAGC                GC^[ACGT]GC
              112  GCAGC               
HapII          16  CCGG                 C^CGG
              195  CCGG                
HpaII          16  CCGG                 C^CGG
              195  CCGG                
ItaI          109  GCAGC                GC^[ACGT]GC
              112  GCAGC               
MboII         201  GAAGA                GAAGA(8/7)
              209  TCTTC               
MslI          142  CACGCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              165  CACCCCTGTG          
MspI           16  CCGG                 C^CGG
              195  CCGG                
NciI           15  CCCGG                CC^[GC]GG
              195  CCGGG               
ScrFI          15  CCCGG                CC^[ACGT]GG
              195  CCGGG               
TseI          109  GCAGC                G^C[AT]GC
              112  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

Asp700I     Cac8I       HphI        MnlI        XmnI