Restriction map for
pF1KB5292
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# NO CUT ENZYMES ( 129 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HaeII
HindIII HpaI KasI Ksp632I KspI
MaeI MfeI MluI MroI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tru9I Tsp509I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 1159 GACGTC GACGT^C
AhdI 460 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 678 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 460 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 208 GTGCTC G[AT]GC[AT]^C
AspI 102 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 714 TGCGCA TGC^GCA
BalI 836 TGGCCA TGG^CCA
BpmI 9 CTCCAG CTGGAG(16/14)
BsaAI 391 TACGTG [CT]AC^GT[GA]
BsaBI 246 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 297 CTCCTC GAGGAG(10/8)
BsiEI 1170 CGGCCG CG[GA][CT]^CG
BsiHKAI 208 GTGCTC G[AT]GC[AT]^C
BsmAI 154 GTCTC GTCTC(1/5)
BsmBI 153 CGTCTC CGTCTC(1/5)
EagI 1170 CGGCCG C^GGCCG
Eam1105I 460 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1170 CGGCCG C^GGCCG
Eco52I 1170 CGGCCG C^GGCCG
FspI 714 TGCGCA TGC^GCA
HincII 622 GTCAAC GT[CT]^[GA]AC
HindII 622 GTCAAC GT[CT]^[GA]AC
MamI 246 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 836 TGGCCA TGG^CCA
MscI 836 TGGCCA TGG^CCA
PflFI 102 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 302 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 751 GACTC GAGTC(4/5)
PstI 931 CTGCAG CTGCA^G
SexAI 824 ACCAGGT A^CC[AT]GGT
SfcI 931 CTGCAG C^T[GA][CT]AG
SfiI 939 GGCCATCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 800 AGGCCT AGG^CCT
TfiI 960 GATTC G^A[AT]TC
Tth111I 102 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 302 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 678 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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Acc65I 403 GGTACC G^GTACC
446 GGTACC
AlwNI 494 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
661 CAGAAGCTG
ApaI 193 GGGCCC GGGCC^C
906 GGGCCC
Asp718I 403 GGTACC G^GTACC
446 GGTACC
AvaI 202 CCCGAG C^[CT]CG[GA]G
429 CTCGGG
BglI 940 GCCATCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1097 GCCTGAGCGGC
BsgI 44 CTGCAC GTGCAG(16/14)
76 CTGCAC
BsoBI 202 CCCGAG C^[CT]CG[GA]G
429 CTCGGG
BspMI 528 GCAGGT ACCTGC(4/8)
810 GCAGGT
BsrBI 22 CCGCTC CCGCTC(-3/-3)
1101 GAGCGG
EaeI 836 TGGCCA [CT]^GGCC[GA]
1170 CGGCCG
HinfI 751 GACTC G^A[ACGT]TC
960 GATTC
KpnI 403 GGTACC GGTAC^C
446 GGTACC
MslI 387 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1110 CATGGGCATG
ScaI 161 AGTACT AGT^ACT
233 AGTACT
SmlI 211 CTCAAG C^T[CT][GA]AG
1066 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BamHI BanI BanII
BbsI BbvI BcnI BmyI BpuAI
BsaHI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I XhoII