Restriction map for
pF1KB4899
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MslI MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1296 GACGTC GACGT^C
AflIII 1313 ACGTGT A^C[GA][CT]GT
AlwNI 409 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1307 AGCGCT AGC^GCT
AspHI 861 GAGCTC G[AT]GC[AT]^C
AvaI 1360 CCCGAG C^[CT]CG[GA]G
BalI 405 TGGCCA TGG^CCA
BanII 861 GAGCTC G[GA]GC[CT]^C
BclI 497 TGATCA T^GATCA
BglI 37 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 861 GAGCTC G[AGT]GC[ACT]^C
BsaAI 1312 TACGTG [CT]AC^GT[GA]
BsiHKAI 861 GAGCTC G[AT]GC[AT]^C
BsmBI 1157 GAGACG CGTCTC(1/5)
BsoBI 1360 CCCGAG C^[CT]CG[GA]G
Bsp1286I 861 GAGCTC G[AGT]GC[ACT]^C
BspHI 180 TCATGA T^CATGA
BspMI 1047 ACCTGC ACCTGC(4/8)
BstBI 940 TTCGAA TT^CGAA
DraII 100 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 133 GAAGAG CTCTTC(1/4)
Eco47III 1307 AGCGCT AGC^GCT
EcoNI 423 CCTTGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 100 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 423 CCTTGG C^C[AT][AT]GG
FauI 1199 CCCGC CCCGC(4/6)
FbaI 497 TGATCA T^GATCA
HincII 210 GTTAAC GT[CT]^[GA]AC
HindII 210 GTTAAC GT[CT]^[GA]AC
HpaI 210 GTTAAC GTT^AAC
Ksp632I 133 GAAGAG CTCTTC(1/4)
MluNI 405 TGGCCA TGG^CCA
MscI 405 TGGCCA TGG^CCA
NaeI 1063 GCCGGC GCC^GGC
NgoMIV 1063 GCCGGC G^CCGGC
NspI 659 GCATGT [GA]CATG^[CT]
NspV 940 TTCGAA TT^CGAA
PpuMI 100 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 180 TCATGA T^CATGA
SacI 861 GAGCTC GAGCT^C
SapI 133 GAAGAGC GCTCTTC(1/4)
SexAI 29 ACCAGGT A^CC[AT]GGT
SfaNI 411 GATGC GCATC(5/9)
SfuI 940 TTCGAA TT^CGAA
SgrAI 1201 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 67 CTCAAG C^T[CT][GA]AG
StyI 423 CCTTGG C^C[AT][AT]GG
XcmI 298 CCAAAGAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 540 AAATTC [GA]^AATT[CT]
789 GAATTT
AfaI 685 GTAC GT^AC
1317 GTAC
ApoI 540 AAATTC [GA]^AATT[CT]
789 GAATTT
Asp700I 3 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
433 GAACGCTTTC
BbeI 284 GGCGCC GGCGC^C
606 GGCGCC
BcnI 1020 CCCGG CC^[GC]GG
1254 CCGGG
BlpI 729 GCTCAGC GC^T[ACGT]AGC
1248 GCTGAGC
Bpu1102I 729 GCTCAGC GC^T[ACGT]AGC
1248 GCTGAGC
BseRI 874 CTCCTC GAGGAG(10/8)
1011 GAGGAG
BsgI 1369 CTGCAC GTGCAG(16/14)
219 GTGCAG
BsmI 617 GAATGC GAATGC(1/-1)
293 GCATTC
BsrFI 1063 GCCGGC [GA]^CCGG[CT]
1202 GCCGGT
BstAPI 138 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
147 GCAAGAAGTGC
CelII 729 GCTCAGC GC^T[ACGT]AGC
1248 GCTGAGC
Cfr10I 1063 GCCGGC [GA]^CCGG[CT]
1202 GCCGGT
DpnII 498 GATC ^GATC
526 GATC
EaeI 405 TGGCCA [CT]^GGCC[GA]
1061 TGGCCG
KasI 284 GGCGCC G^GCGCC
606 GGCGCC
MboI 498 GATC ^GATC
526 GATC
NarI 284 GGCGCC GG^CGCC
606 GGCGCC
NciI 1020 CCCGG CC^[GC]GG
1254 CCGGG
NdeII 498 GATC ^GATC
526 GATC
RsaI 685 GTAC GT^AC
1317 GTAC
Sau3AI 498 GATC ^GATC
526 GATC
SfoI 284 GGCGCC GGC^GCC
606 GGCGCC
XmnI 3 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
433 GAACGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BbvI BpmI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI NlaIII
NlaIV PleI PstI PvuII Sau96I
ScrFI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I