Restriction map for
pF1KE5408
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI DsaI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MflI MluI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 318 CGCG CG^CG
AflIII 748 ACGTGT A^C[GA][CT]GT
AhdI 889 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 819 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 889 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 288 CTCGGG C^[CT]CG[GA]G
AviII 167 TGCGCA TGC^GCA
BanI 47 GGCACC G^G[CT][GA]CC
BanII 727 GAGCTC G[GA]GC[CT]^C
BbrPI 747 CACGTG CAC^GTG
BlpI 276 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 276 GCTCAGC GC^T[ACGT]AGC
BsaAI 747 CACGTG [CT]AC^GT[GA]
BsaBI 738 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 596 CGGCCG CG[GA][CT]^CG
BsmI 422 GCATTC GAATGC(1/-1)
BsoBI 288 CTCGGG C^[CT]CG[GA]G
BstAPI 20 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 318 CGCG CG^CG
CelII 276 GCTCAGC GC^T[ACGT]AGC
DraII 146 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 364 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 596 CGGCCG C^GGCCG
Eam1105I 889 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 322 CTCTTC CTCTTC(1/4)
EclXI 596 CGGCCG C^GGCCG
Eco52I 596 CGGCCG C^GGCCG
EcoNI 697 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 146 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 558 CCTTGG C^C[AT][AT]GG
FauI 482 CCCGC CCCGC(4/6)
FspI 167 TGCGCA TGC^GCA
HgaI 182 GACGC GACGC(5/10)
HinfI 640 GAATC G^A[ACGT]TC
Ksp632I 322 CTCTTC CTCTTC(1/4)
MamI 738 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 465 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 318 CGCG CG^CG
NaeI 16 GCCGGC GCC^GGC
NgoMIV 16 GCCGGC G^CCGGC
NlaIV 47 GGCACC GG[ACGT]^[ACGT]CC
PmaCI 747 CACGTG CAC^GTG
PmlI 747 CACGTG CAC^GTG
PpuMI 146 AGGACCT [GA]G^G[AT]CC[CT]
PstI 4 CTGCAG CTGCA^G
SacI 727 GAGCTC GAGCT^C
SapI 321 GCTCTTC GCTCTTC(1/4)
SexAI 98 ACCTGGT A^CC[AT]GGT
SfcI 4 CTGCAG C^T[GA][CT]AG
StuI 392 AGGCCT AGG^CCT
StyI 558 CCTTGG C^C[AT][AT]GG
TaqI 907 TCGA T^CGA
TfiI 640 GAATC G^A[AT]TC
TthHB8I 907 TCGA T^CGA
XcmI 524 CCAAGCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 819 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 718 AAATTC [GA]^AATT[CT]
823 AAATTC
Alw44I 472 GTGCAC G^TGCAC
871 GTGCAC
AlwI 743 GGATC GGATC(4/5)
864 GGATC
ApaLI 472 GTGCAC G^TGCAC
871 GTGCAC
ApoI 718 AAATTC [GA]^AATT[CT]
823 AAATTC
BcnI 240 CCGGG CC^[GC]GG
594 CCCGG
BsmFI 305 GGGAC GGGAC(10/14)
9 GTCCC
BsrBI 51 CCGCTC CCGCTC(-3/-3)
43 GAGCGG
BsrFI 16 GCCGGC [GA]^CCGG[CT]
489 GCCGGT
BssSI 199 CACGAG CACGAG(-5/-1)
24 CTCGTG
Cfr10I 16 GCCGGC [GA]^CCGG[CT]
489 GCCGGT
MaeII 476 ACGT A^CGT
748 ACGT
NciI 240 CCGGG CC^[GC]GG
594 CCCGG
PvuII 220 CAGCTG CAG^CTG
923 CAGCTG
SfaNI 401 GCATC GCATC(5/9)
464 GCATC
Tsp45I 478 GTCAC ^GT[GC]AC
568 GTCAC
Tsp509I 719 AATT ^AATT
824 AATT
TspRI 113 CAGTG CA[GC]TG[ACGT][ACGT]^
570 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BalI BbsI BbvI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI TseI