################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspLU11I BspMI BsrBI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I FauI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MscI MspA1I MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 304 TCCGGA T^CCGGA AfaI 454 GTAC GT^AC AluI 84 AGCT AG^CT AlwNI 386 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 26 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BclI 341 TGATCA T^GATCA BfaI 349 CTAG C^TAG BglII 183 AGATCT A^GATCT BpmI 415 CTGGAG CTGGAG(16/14) BsaWI 304 TCCGGA [AT]^CCGG[AT] BseAI 304 TCCGGA T^CCGGA BsgI 119 GTGCAG GTGCAG(16/14) BsmI 89 GCATTC GAATGC(1/-1) Bsp1407I 453 TGTACA T^GTACA BspEI 304 TCCGGA T^CCGGA BspHI 321 TCATGA T^CATGA BsrDI 259 GCAATG GCAATG(2/0) BsrGI 453 TGTACA T^GTACA BstBI 9 TTCGAA TT^CGAA BstYI 183 AGATCT [GA]^GATC[CT] Cac8I 85 GCTGGC GC[ACGT]^[ACGT]GC EcoT22I 199 ATGCAT ATGCA^T FbaI 341 TGATCA T^GATCA HapII 305 CCGG C^CGG HpaII 305 CCGG C^CGG MaeI 349 CTAG C^TAG MflI 183 AGATCT [GA]^GATC[CT] MroI 304 TCCGGA T^CCGGA MseI 431 TTAA T^TAA MslI 202 CATATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 305 CCGG C^CGG NsiI 199 ATGCAT ATGCA^T NspV 9 TTCGAA TT^CGAA PleI 301 GAGTC GAGTC(4/5) RcaI 321 TCATGA T^CATGA RsaI 454 GTAC GT^AC SfuI 9 TTCGAA TT^CGAA SmlI 298 CTTGAG C^T[CT][GA]AG SspBI 453 TGTACA T^GTACA SspI 427 AATATT AAT^ATT TaqI 10 TCGA T^CGA TfiI 388 GAATC G^A[AT]TC Tru9I 431 TTAA T^TAA TthHB8I 10 TCGA T^CGA XhoII 183 AGATCT [GA]^GATC[CT] XmnI 26 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 5 GAATTT [GA]^AATT[CT] 436 GAATTT ApoI 5 GAATTT [GA]^AATT[CT] 436 GAATTT BbsI 97 GTCTTC GAAGAC(2/6) 366 GTCTTC BpuAI 97 GTCTTC GAAGAC(2/6) 366 GTCTTC DpnII 184 GATC ^GATC 342 GATC HinfI 301 GAGTC G^A[ACGT]TC 388 GAATC HphI 150 GGTGA GGTGA(8/7) 237 TCACC MboI 184 GATC ^GATC 342 GATC MnlI 409 GAGG CCTC(7/6) 418 GAGG NdeII 184 GATC ^GATC 342 GATC NlaIII 94 CATG CATG^ 322 CATG Sau3AI 184 GATC ^GATC 342 GATC TspRI 40 CAGTG CA[GC]TG[ACGT][ACGT]^ 117 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BsrI BstF5I FokI MaeIII MboII SfaNI Tsp509I