Restriction map for pF1KB6819
##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BcnI        BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspDI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstEII      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
FauI        Fnu4HI      FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        ItaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MluI        MluNI       MscI        
MspA1I      MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        StyI        SwaI        TseI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        304  TCCGGA               T^CCGGA
AfaI          454  GTAC                 GT^AC
AluI           84  AGCT                 AG^CT
AlwNI         386  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I        26  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI          341  TGATCA               T^GATCA
BfaI          349  CTAG                 C^TAG
BglII         183  AGATCT               A^GATCT
BpmI          415  CTGGAG               CTGGAG(16/14)
BsaWI         304  TCCGGA               [AT]^CCGG[AT]
BseAI         304  TCCGGA               T^CCGGA
BsgI          119  GTGCAG               GTGCAG(16/14)
BsmI           89  GCATTC               GAATGC(1/-1)
Bsp1407I      453  TGTACA               T^GTACA
BspEI         304  TCCGGA               T^CCGGA
BspHI         321  TCATGA               T^CATGA
BsrDI         259  GCAATG               GCAATG(2/0)
BsrGI         453  TGTACA               T^GTACA
BstBI           9  TTCGAA               TT^CGAA
BstYI         183  AGATCT               [GA]^GATC[CT]
Cac8I          85  GCTGGC               GC[ACGT]^[ACGT]GC
EcoT22I       199  ATGCAT               ATGCA^T
FbaI          341  TGATCA               T^GATCA
HapII         305  CCGG                 C^CGG
HpaII         305  CCGG                 C^CGG
MaeI          349  CTAG                 C^TAG
MflI          183  AGATCT               [GA]^GATC[CT]
MroI          304  TCCGGA               T^CCGGA
MseI          431  TTAA                 T^TAA
MslI          202  CATATTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          305  CCGG                 C^CGG
NsiI          199  ATGCAT               ATGCA^T
NspV            9  TTCGAA               TT^CGAA
PleI          301  GAGTC                GAGTC(4/5)
RcaI          321  TCATGA               T^CATGA
RsaI          454  GTAC                 GT^AC
SfuI            9  TTCGAA               TT^CGAA
SmlI          298  CTTGAG               C^T[CT][GA]AG
SspBI         453  TGTACA               T^GTACA
SspI          427  AATATT               AAT^ATT
TaqI           10  TCGA                 T^CGA
TfiI          388  GAATC                G^A[AT]TC
Tru9I         431  TTAA                 T^TAA
TthHB8I        10  TCGA                 T^CGA
XhoII         183  AGATCT               [GA]^GATC[CT]
XmnI           26  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AcsI            5  GAATTT               [GA]^AATT[CT]
              436  GAATTT              
ApoI            5  GAATTT               [GA]^AATT[CT]
              436  GAATTT              
BbsI           97  GTCTTC               GAAGAC(2/6)
              366  GTCTTC              
BpuAI          97  GTCTTC               GAAGAC(2/6)
              366  GTCTTC              
DpnII         184  GATC                 ^GATC
              342  GATC                
HinfI         301  GAGTC                G^A[ACGT]TC
              388  GAATC               
HphI          150  GGTGA                GGTGA(8/7)
              237  TCACC               
MboI          184  GATC                 ^GATC
              342  GATC                
MnlI          409  GAGG                 CCTC(7/6)
              418  GAGG                
NdeII         184  GATC                 ^GATC
              342  GATC                
NlaIII         94  CATG                 CATG^
              322  CATG                
Sau3AI        184  GATC                 ^GATC
              342  GATC                
TspRI          40  CAGTG                CA[GC]TG[ACGT][ACGT]^
              117  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BsrI        BstF5I      FokI        MaeIII      MboII       
SfaNI       Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com