Restriction map for
pF1KB6254
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbrPI BciVI BclI BfrI
BglII BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
DraI DraIII Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KspI MamI
MluI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI Van91I
XcmI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1589 GGTACC G^GTACC
AcsI 958 GAATTT [GA]^AATT[CT]
AcyI 1208 GGCGCC G[GA]^CG[CT]C
AgeI 233 ACCGGT A^CCGGT
ApoI 958 GAATTT [GA]^AATT[CT]
Asp700I 162 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1589 GGTACC G^GTACC
AspI 1567 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1208 GGCGCC GGCGC^C
BbsI 1990 GTCTTC GAAGAC(2/6)
BbvCI 360 CCTCAGC CCTCAGC(-5/-2)
BcgI 56 CGACCTCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1034 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1034 GCTCAGC GC^T[ACGT]AGC
BpuAI 1990 GTCTTC GAAGAC(2/6)
BsaAI 1996 TACGTG [CT]AC^GT[GA]
BsaHI 1208 GGCGCC G[GA]^CG[CT]C
BstAPI 1393 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1034 GCTCAGC GC^T[ACGT]AGC
CpoI 1906 CGGACCG CG^G[AT]CCG
EcoT14I 326 CCTTGG C^C[AT][AT]GG
Hin1I 1208 GGCGCC G[GA]^CG[CT]C
HincII 948 GTCAAC GT[CT]^[GA]AC
HindII 948 GTCAAC GT[CT]^[GA]AC
KasI 1208 GGCGCC G^GCGCC
KpnI 1589 GGTACC GGTAC^C
MfeI 300 CAATTG C^AATTG
MslI 1752 CATCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 300 CAATTG C^AATTG
NaeI 1188 GCCGGC GCC^GGC
NarI 1208 GGCGCC GG^CGCC
NgoMIV 1188 GCCGGC G^CCGGC
PflFI 1567 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 233 ACCGGT A^CCGGT
RsrII 1906 CGGACCG CG^G[AT]CCG
SacI 508 GAGCTC GAGCT^C
SbfI 684 CCTGCAGG CCTGCA^GG
SfaNI 311 GATGC GCATC(5/9)
SfoI 1208 GGCGCC GGC^GCC
Sse8387I 684 CCTGCAGG CCTGCA^GG
StyI 326 CCTTGG C^C[AT][AT]GG
Tsp45I 2023 GTCAC ^GT[GC]AC
Tth111I 1567 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1821 TCTAGA T^CTAGA
XmnI 162 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 566 TCCGGA T^CCGGA
977 TCCGGA
AspHI 508 GAGCTC G[AT]GC[AT]^C
1863 GTGCTC
BfaI 112 CTAG C^TAG
1822 CTAG
BseAI 566 TCCGGA T^CCGGA
977 TCCGGA
BsgI 98 GTGCAG GTGCAG(16/14)
1054 GTGCAG
BsiEI 8 CGGCCG CG[GA][CT]^CG
1732 CGGCCG
BsiHKAI 508 GAGCTC G[AT]GC[AT]^C
1863 GTGCTC
BsmBI 645 CGTCTC CGTCTC(1/5)
90 GAGACG
BspEI 566 TCCGGA T^CCGGA
977 TCCGGA
DrdI 556 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1072 GACAGCCATGTC
DsaI 122 CCGTGG C^C[GA][CT]GG
1483 CCACGG
EagI 8 CGGCCG C^GGCCG
1732 CGGCCG
EclXI 8 CGGCCG C^GGCCG
1732 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
1732 CGGCCG
HaeII 155 GGCGCT [GA]GCGC^[CT]
1208 GGCGCC
HgaI 92 GACGC GACGC(5/10)
644 GCGTC
MaeI 112 CTAG C^TAG
1822 CTAG
MaeII 494 ACGT A^CGT
1997 ACGT
MaeIII 638 GTAAC ^GT[ACGT]AC
2023 GTCAC
MroI 566 TCCGGA T^CCGGA
977 TCCGGA
MseI 1135 TTAA T^TAA
1618 TTAA
SapI 595 GAAGAGC GCTCTTC(1/4)
1096 GAAGAGC
TaqI 1070 TCGA T^CGA
1833 TCGA
TfiI 333 GAATC G^A[AT]TC
653 GAATC
Tru9I 1135 TTAA T^TAA
1618 TTAA
TthHB8I 1070 TCGA T^CGA
1833 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AvaI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI SmaI SmlI TseI
Tsp509I TspRI XhoII XmaCI XmaI