##################################
# NO CUT ENZYMES (  221 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        Alw44I      AlwI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      Sau96I      SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AhdI          163  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          206  AGCT                 AG^CT
AlwNI         218  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           34  CCTGAGG              CC^T[ACGT]AGG
AspEI         163  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII         177  GAGCCC               G[GA]GC[CT]^C
BciVI         119  GTATCC               GTATCC(6/5)
BmyI          177  GAGCCC               G[AGT]GC[ACT]^C
BpmI          165  CTCCAG               CTGGAG(16/14)
BsaJI         222  CCCTGG               C^C[ACGT][ACGT]GG
Bsp1286I      177  GAGCCC               G[AGT]GC[ACT]^C
BspMI         184  ACCTGC               ACCTGC(4/8)
Bsu36I         34  CCTGAGG              CC^T[ACGT]AGG
Eam1105I      163  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          213  CTCTTC               CTCTTC(1/4)
Eco81I         34  CCTGAGG              CC^T[ACGT]AGG
HphI          144  GGTGA                GGTGA(8/7)
Ksp632I       213  CTCTTC               CTCTTC(1/4)
MboII         214  TCTTC                GAAGA(8/7)
MwoI          179  GCCCAACCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII         87  CATG                 CATG^


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BsgI           73  GTGCAG               GTGCAG(16/14)
              150  GTGCAG              
BsiYI          21  CCCCACAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              223  CCTGGTGGCGG         
BslI           21  CCCCACAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              223  CCTGGTGGCGG         
BssKI         167  CCAGG                ^CC[ACGT]GG
              223  CCTGG               
BstNI         167  CCAGG                CC^[AT]GG
              223  CCTGG               
EcoRII        167  CCAGG                ^CC[AT]GG
              223  CCTGG               
Fnu4HI        230  GCGGC                GC^[ACGT]GC
              233  GCGGC               
HinfI          90  GACTC                G^A[ACGT]TC
              163  GACTC               
ItaI          230  GCGGC                GC^[ACGT]GC
              233  GCGGC               
MvaI          167  CCAGG                CC^[AT]GG
              223  CCTGG               
NlaIV          30  GGCTCC               GG[ACGT]^[ACGT]CC
               39  GGAACC              
PleI           90  GACTC                GAGTC(4/5)
              163  GACTC               
ScrFI         167  CCAGG                CC^[ACGT]GG
              223  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AciI        DdeI        MnlI