Restriction map for
pF1KE0181
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbvCI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HaeII HapII HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccI 679 GTCTAC GT^[AC][GT]AC
AlwNI 67 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 765 GGGCCC GGGCC^C
AscI 84 GGCGCGCC GG^CGCGCC
AspHI 450 GAGCTC G[AT]GC[AT]^C
AspI 723 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 668 GGACC G^G[AT]CC
BalI 584 TGGCCA TGG^CCA
BbrPI 79 CACGTG CAC^GTG
BbsI 208 GTCTTC GAAGAC(2/6)
BcgI 257 GCAGGAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 98 TGATCA T^GATCA
BfaI 391 CTAG C^TAG
BpmI 112 CTGGAG CTGGAG(16/14)
BpuAI 208 GTCTTC GAAGAC(2/6)
BsaAI 79 CACGTG [CT]AC^GT[GA]
BseRI 371 GAGGAG GAGGAG(10/8)
BsgI 268 GTGCAG GTGCAG(16/14)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsiHKAI 450 GAGCTC G[AT]GC[AT]^C
BsmAI 778 GTCTC GTCTC(1/5)
BspHI 506 TCATGA T^CATGA
BssHII 85 GCGCGC G^CGCGC
BstAPI 66 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 99 GATC ^GATC
DraI 595 TTTAAA TTT^AAA
DraII 765 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 782 CCATGG C^C[GA][CT]GG
EagI 26 CGGCCG C^GGCCG
EarI 414 GAAGAG CTCTTC(1/4)
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoO109I 765 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 782 CCATGG C^C[AT][AT]GG
EcoT22I 691 ATGCAT ATGCA^T
FauI 427 GCGGG CCCGC(4/6)
FbaI 98 TGATCA T^GATCA
HphI 732 GGTGA GGTGA(8/7)
Ksp632I 414 GAAGAG CTCTTC(1/4)
MaeI 391 CTAG C^TAG
MaeIII 58 GTCAC ^GT[ACGT]AC
MboI 99 GATC ^GATC
MluNI 584 TGGCCA TGG^CCA
MscI 584 TGGCCA TGG^CCA
MseI 596 TTAA T^TAA
MspA1I 229 CAGCTG C[AC]G^C[GT]G
NcoI 782 CCATGG C^CATGG
NdeII 99 GATC ^GATC
NsiI 691 ATGCAT ATGCA^T
PflFI 723 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 618 GACTC GAGTC(4/5)
PmaCI 79 CACGTG CAC^GTG
PmlI 79 CACGTG CAC^GTG
PstI 307 CTGCAG CTGCA^G
PvuII 229 CAGCTG CAG^CTG
RcaI 506 TCATGA T^CATGA
SacI 450 GAGCTC GAGCT^C
SapI 414 GAAGAGC GCTCTTC(1/4)
Sau3AI 99 GATC ^GATC
SfaNI 122 GCATC GCATC(5/9)
StuI 375 AGGCCT AGG^CCT
StyI 782 CCATGG C^C[AT][AT]GG
TaqI 164 TCGA T^CGA
Tru9I 596 TTAA T^TAA
Tsp45I 58 GTCAC ^GT[GC]AC
TspRI 139 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 723 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 164 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 86 CGCG CG^CG
154 CGCG
AciI 182 GCGG CCGC(-3/-1)
427 GCGG
AcyI 430 GGCGTC G[GA]^CG[CT]C
526 GACGCC
BsaHI 430 GGCGTC G[GA]^CG[CT]C
526 GACGCC
BspMI 71 ACCTGC ACCTGC(4/8)
188 ACCTGC
BstF5I 123 CATCC GGATG(2/0)
786 GGATG
BstUI 86 CGCG CG^CG
154 CGCG
EaeI 26 CGGCCG [CT]^GGCC[GA]
584 TGGCCA
FokI 123 CATCC GGATG(9/13)
786 GGATG
Hin1I 430 GGCGTC G[GA]^CG[CT]C
526 GACGCC
MaeII 80 ACGT A^CGT
647 ACGT
MslI 123 CATCCGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
726 CATGTCGGTG
MvnI 86 CGCG CG^CG
154 CGCG
NspI 419 GCATGT [GA]CATG^[CT]
467 ACATGC
SfcI 307 CTGCAG C^T[GA][CT]AG
321 CTACAG
TfiI 296 GAATC G^A[AT]TC
397 GAATC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BanII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I EcoRII Fnu4HI HaeIII HgaI
HhaI HinP1I HinfI ItaI MboII
MnlI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI TseI