Restriction map for pF1KB6792
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AlwI        AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeIII      
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp509I     Tth111I     XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI          306  GCGG                 CCGC(-3/-1)
Alw44I        363  GTGCAC               G^TGCAC
AlwNI         196  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         363  GTGCAC               G^TGCAC
BcgI          333  CGAGTCGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmI          426  GAATGC               GAATGC(1/-1)
BsrFI         302  ACCGGC               [GA]^CCGG[CT]
CfoI           16  GCGC                 GCG^C
Cfr10I        302  ACCGGC               [GA]^CCGG[CT]
DdeI            7  CTGAG                C^T[ACGT]AG
EarI          118  GAAGAG               CTCTTC(1/4)
EcoT14I       275  CCAAGG               C^C[AT][AT]GG
FauI          306  GCGGG                CCCGC(4/6)
HaeII          15  GGCGCT               [GA]GCGC^[CT]
HhaI           16  GCGC                 GCG^C
HinP1I         16  GCGC                 G^CGC
Ksp632I       118  GAAGAG               CTCTTC(1/4)
MaeIII        461  GTGAC                ^GT[ACGT]AC
MboII         118  GAAGA                GAAGA(8/7)
MseI          110  TTAA                 T^TAA
MwoI           54  GCTTTATCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI           93  GCATGC               [GA]CATG^[CT]
PflMI         137  CCAATCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          259  GAGCTC               GAGCT^C
SfaNI         151  GATGC                GCATC(5/9)
SmaI          348  CCCGGG               CCC^GGG
SphI           93  GCATGC               GCATG^C
StyI          275  CCAAGG               C^C[AT][AT]GG
TaqI          332  TCGA                 T^CGA
TfiI          280  GAATC                G^A[AT]TC
Tru9I         110  TTAA                 T^TAA
Tsp45I        461  GTGAC                ^GT[GC]AC
TthHB8I       332  TCGA                 T^CGA
Van91I        137  CCAATCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         348  CCCGGG               C^CCGGG
XmaI          348  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AspHI         259  GAGCTC               G[AT]GC[AT]^C
              363  GTGCAC              
AvaI          348  CCCGGG               C^[CT]CG[GA]G
              409  CCCGAG              
AvaII           3  GGTCC                G^G[AT]CC
              132  GGTCC               
BanI          271  GGTGCC               G^G[CT][GA]CC
              369  GGTGCC              
BanII         259  GAGCTC               G[GA]GC[CT]^C
              316  GGGCTC              
BbvI           90  GCTGC                GCAGC(8/12)
              340  GCTGC               
BcnI          348  CCCGG                CC^[GC]GG
              349  CCGGG               
BfaI           79  CTAG                 C^TAG
              238  CTAG                
BpmI          324  CTCCAG               CTGGAG(16/14)
               83  CTGGAG              
BsiHKAI       259  GAGCTC               G[AT]GC[AT]^C
              363  GTGCAC              
BsoBI         348  CCCGGG               C^[CT]CG[GA]G
              409  CCCGAG              
BsrI          195  CCAGT                ACTGG(1/-1)
              244  CCAGT               
BstF5I        171  CATCC                GGATG(2/0)
              150  GGATG               
BstNI         174  CCTGG                CC^[AT]GG
              183  CCAGG               
Cac8I          93  GCATGC               GC[ACGT]^[ACGT]GC
              154  GCCAGC              
Cfr13I          3  GGTCC                G^G[ACGT]CC
              132  GGTCC               
DpnII         123  GATC                 ^GATC
              382  GATC                
EcoRII        174  CCTGG                ^CC[AT]GG
              183  CCAGG               
Fnu4HI         90  GCTGC                GC^[ACGT]GC
              340  GCTGC               
FokI          171  CATCC                GGATG(9/13)
              150  GGATG               
HapII         303  CCGG                 C^CGG
              349  CCGG                
HpaII         303  CCGG                 C^CGG
              349  CCGG                
ItaI           90  GCTGC                GC^[ACGT]GC
              340  GCTGC               
MaeI           79  CTAG                 C^TAG
              238  CTAG                
MboI          123  GATC                 ^GATC
              382  GATC                
MnlI          323  CCTC                 CCTC(7/6)
               86  GAGG                
MspI          303  CCGG                 C^CGG
              349  CCGG                
MvaI          174  CCTGG                CC^[AT]GG
              183  CCAGG               
NciI          348  CCCGG                CC^[GC]GG
              349  CCGGG               
NdeII         123  GATC                 ^GATC
              382  GATC                
Sau3AI        123  GATC                 ^GATC
              382  GATC                
Sau96I          3  GGTCC                G^G[ACGT]CC
              132  GGTCC               
TseI           90  GCTGC                G^C[AT]GC
              340  GCTGC               
XcmI           72  CCAGCTTCTAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              137  CCAATCGGTGGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BmyI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BssKI       HinfI       HphI        
NlaIII      NlaIV       PleI        ScrFI       TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com