################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeIII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 306 GCGG CCGC(-3/-1) Alw44I 363 GTGCAC G^TGCAC AlwNI 196 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 363 GTGCAC G^TGCAC BcgI 333 CGAGTCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsmI 426 GAATGC GAATGC(1/-1) BsrFI 302 ACCGGC [GA]^CCGG[CT] CfoI 16 GCGC GCG^C Cfr10I 302 ACCGGC [GA]^CCGG[CT] DdeI 7 CTGAG C^T[ACGT]AG EarI 118 GAAGAG CTCTTC(1/4) EcoT14I 275 CCAAGG C^C[AT][AT]GG FauI 306 GCGGG CCCGC(4/6) HaeII 15 GGCGCT [GA]GCGC^[CT] HhaI 16 GCGC GCG^C HinP1I 16 GCGC G^CGC Ksp632I 118 GAAGAG CTCTTC(1/4) MaeIII 461 GTGAC ^GT[ACGT]AC MboII 118 GAAGA GAAGA(8/7) MseI 110 TTAA T^TAA MwoI 54 GCTTTATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 93 GCATGC [GA]CATG^[CT] PflMI 137 CCAATCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 259 GAGCTC GAGCT^C SfaNI 151 GATGC GCATC(5/9) SmaI 348 CCCGGG CCC^GGG SphI 93 GCATGC GCATG^C StyI 275 CCAAGG C^C[AT][AT]GG TaqI 332 TCGA T^CGA TfiI 280 GAATC G^A[AT]TC Tru9I 110 TTAA T^TAA Tsp45I 461 GTGAC ^GT[GC]AC TthHB8I 332 TCGA T^CGA Van91I 137 CCAATCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 348 CCCGGG C^CCGGG XmaI 348 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AspHI 259 GAGCTC G[AT]GC[AT]^C 363 GTGCAC AvaI 348 CCCGGG C^[CT]CG[GA]G 409 CCCGAG AvaII 3 GGTCC G^G[AT]CC 132 GGTCC BanI 271 GGTGCC G^G[CT][GA]CC 369 GGTGCC BanII 259 GAGCTC G[GA]GC[CT]^C 316 GGGCTC BbvI 90 GCTGC GCAGC(8/12) 340 GCTGC BcnI 348 CCCGG CC^[GC]GG 349 CCGGG BfaI 79 CTAG C^TAG 238 CTAG BpmI 324 CTCCAG CTGGAG(16/14) 83 CTGGAG BsiHKAI 259 GAGCTC G[AT]GC[AT]^C 363 GTGCAC BsoBI 348 CCCGGG C^[CT]CG[GA]G 409 CCCGAG BsrI 195 CCAGT ACTGG(1/-1) 244 CCAGT BstF5I 171 CATCC GGATG(2/0) 150 GGATG BstNI 174 CCTGG CC^[AT]GG 183 CCAGG Cac8I 93 GCATGC GC[ACGT]^[ACGT]GC 154 GCCAGC Cfr13I 3 GGTCC G^G[ACGT]CC 132 GGTCC DpnII 123 GATC ^GATC 382 GATC EcoRII 174 CCTGG ^CC[AT]GG 183 CCAGG Fnu4HI 90 GCTGC GC^[ACGT]GC 340 GCTGC FokI 171 CATCC GGATG(9/13) 150 GGATG HapII 303 CCGG C^CGG 349 CCGG HpaII 303 CCGG C^CGG 349 CCGG ItaI 90 GCTGC GC^[ACGT]GC 340 GCTGC MaeI 79 CTAG C^TAG 238 CTAG MboI 123 GATC ^GATC 382 GATC MnlI 323 CCTC CCTC(7/6) 86 GAGG MspI 303 CCGG C^CGG 349 CCGG MvaI 174 CCTGG CC^[AT]GG 183 CCAGG NciI 348 CCCGG CC^[GC]GG 349 CCGGG NdeII 123 GATC ^GATC 382 GATC Sau3AI 123 GATC ^GATC 382 GATC Sau96I 3 GGTCC G^G[ACGT]CC 132 GGTCC TseI 90 GCTGC G^C[AT]GC 340 GCTGC XcmI 72 CCAGCTTCTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 137 CCAATCGGTGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI HinfI HphI NlaIII NlaIV PleI ScrFI TspRI