Restriction map for
pF1KE0705
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 3 GTCTAC GT^[AC][GT]AC
AfaI 613 GTAC GT^AC
AlwI 658 GATCC GGATC(4/5)
AlwNI 662 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 330 GGGCCC GGGCC^C
AvaII 744 GGACC G^G[AT]CC
BanII 330 GGGCCC G[GA]GC[CT]^C
BfaI 182 CTAG C^TAG
BmyI 330 GGGCCC G[AGT]GC[ACT]^C
BpmI 39 CTCCAG CTGGAG(16/14)
BseRI 401 CTCCTC GAGGAG(10/8)
BsiYI 709 CCACCCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 709 CCACCCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 330 GGGCCC G[AGT]GC[ACT]^C
BspHI 696 TCATGA T^CATGA
BsrDI 868 GCAATG GCAATG(2/0)
BssKI 661 CCAGG ^CC[ACGT]GG
BstNI 661 CCAGG CC^[AT]GG
BstXI 574 CCACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 49 GCCAGC GC[ACGT]^[ACGT]GC
DraII 330 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 309 CCATGG C^C[GA][CT]GG
EcoO109I 330 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 661 CCAGG ^CC[AT]GG
EcoRV 542 GATATC GAT^ATC
HapII 619 CCGG C^CGG
HincII 634 GTTAAC GT[CT]^[GA]AC
HindII 634 GTTAAC GT[CT]^[GA]AC
HindIII 171 AAGCTT A^AGCTT
HpaI 634 GTTAAC GTT^AAC
HpaII 619 CCGG C^CGG
HphI 520 TCACC GGTGA(8/7)
MaeI 182 CTAG C^TAG
MaeIII 811 GTTAC ^GT[ACGT]AC
MspI 619 CCGG C^CGG
MvaI 661 CCAGG CC^[AT]GG
MwoI 622 GCTTATTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 309 CCATGG C^CATGG
NlaIV 330 GGGCCC GG[ACGT]^[ACGT]CC
PflMI 709 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 696 TCATGA T^CATGA
RsaI 613 GTAC GT^AC
ScrFI 661 CCAGG CC^[ACGT]GG
SmlI 815 CTCAAG C^T[CT][GA]AG
TaqI 222 TCGA T^CGA
TthHB8I 222 TCGA T^CGA
Van91I 709 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BsaJI 81 CCAAGG C^C[ACGT][ACGT]GG
309 CCATGG
BsgI 277 GTGCAG GTGCAG(16/14)
370 GTGCAG
DpnII 220 GATC ^GATC
658 GATC
EcoT14I 81 CCAAGG C^C[AT][AT]GG
309 CCATGG
HinfI 292 GAATC G^A[ACGT]TC
474 GAATC
MaeII 269 ACGT A^CGT
378 ACGT
MboI 220 GATC ^GATC
658 GATC
MslI 575 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
643 CACTGTAATG
MspA1I 68 CAGCTG C[AC]G^C[GT]G
629 CAGCTG
NdeII 220 GATC ^GATC
658 GATC
PvuII 68 CAGCTG CAG^CTG
629 CAGCTG
Sau3AI 220 GATC ^GATC
658 GATC
SfaNI 570 GATGC GCATC(5/9)
846 GCATC
StyI 81 CCAAGG C^C[AT][AT]GG
309 CCATGG
TfiI 292 GAATC G^A[AT]TC
474 GAATC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsrI
BstF5I Cfr13I DdeI Fnu4HI FokI
HaeIII ItaI MboII MnlI MseI
NlaIII Sau96I Tru9I TseI Tsp509I
TspRI