##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI AscI AseI
AsnI Asp700I AspEI AvrII BbsI
BfrI BglI BglII BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoT22I FseI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MroI MseI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tru9I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 412 GGTACC G^GTACC
Aor51HI 1939 AGCGCT AGC^GCT
Asp718I 412 GGTACC G^GTACC
AspI 2424 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2312 TGCGCA TGC^GCA
BamHI 213 GGATCC G^GATCC
BbvCI 570 CCTCAGC CCTCAGC(-5/-2)
BcgI 939 CGAGGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1917 GGATAC GTATCC(6/5)
BclI 1329 TGATCA T^GATCA
BlpI 402 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC
BseRI 259 GAGGAG GAGGAG(10/8)
BsmI 846 GAATGC GAATGC(1/-1)
BspHI 1575 TCATGA T^CATGA
BspMI 905 ACCTGC ACCTGC(4/8)
BsrFI 1735 GCCGGC [GA]^CCGG[CT]
CelII 402 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1735 GCCGGC [GA]^CCGG[CT]
DraIII 1114 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 436 CTCTTC CTCTTC(1/4)
Eco47III 1939 AGCGCT AGC^GCT
EcoRI 927 GAATTC G^AATTC
EcoRV 1321 GATATC GAT^ATC
FbaI 1329 TGATCA T^GATCA
FspI 2312 TGCGCA TGC^GCA
HgaI 1200 GACGC GACGC(5/10)
KpnI 412 GGTACC GGTAC^C
Ksp632I 436 CTCTTC CTCTTC(1/4)
NaeI 1735 GCCGGC GCC^GGC
NgoMIV 1735 GCCGGC G^CCGGC
PaeR7I 1435 CTCGAG C^TCGAG
PflFI 2424 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1575 TCATGA T^CATGA
SbfI 1866 CCTGCAGG CCTGCA^GG
Sse8387I 1866 CCTGCAGG CCTGCA^GG
Tth111I 2424 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1435 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1873 GGCGCC G[GA]^CG[CT]C
2147 GGCGCC
ApaI 198 GGGCCC GGGCC^C
245 GGGCCC
BbeI 1873 GGCGCC GGCGC^C
2147 GGCGCC
BsaHI 1873 GGCGCC G[GA]^CG[CT]C
2147 GGCGCC
BspLU11I 309 ACATGT A^CATGT
533 ACATGT
BstAPI 1078 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2304 GCAGTGCATGC
BstYI 213 GGATCC [GA]^GATC[CT]
483 GGATCT
DrdI 1018 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1065 GACTAGACTGTC
Hin1I 1873 GGCGCC G[GA]^CG[CT]C
2147 GGCGCC
KasI 1873 GGCGCC G^GCGCC
2147 GGCGCC
MflI 213 GGATCC [GA]^GATC[CT]
483 GGATCT
MslI 708 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
784 CACTGGAGTG
NarI 1873 GGCGCC GG^CGCC
2147 GGCGCC
PstI 65 CTGCAG CTGCA^G
1867 CTGCAG
PvuII 541 CAGCTG CAG^CTG
622 CAGCTG
SacI 395 GAGCTC GAGCT^C
1039 GAGCTC
SexAI 362 ACCAGGT A^CC[AT]GGT
1050 ACCAGGT
SfoI 1873 GGCGCC GGC^GCC
2147 GGCGCC
SmaI 828 CCCGGG CCC^GGG
1393 CCCGGG
SphI 2309 GCATGC GCATG^C
2336 GCATGC
StuI 418 AGGCCT AGG^CCT
656 AGGCCT
XcmI 674 CCACTGCCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2154 CCAGACTCTCAATGG
XhoII 213 GGATCC [GA]^GATC[CT]
483 GGATCT
XmaCI 828 CCCGGG C^CCGGG
1393 CCCGGG
XmaI 828 CCCGGG C^CCGGG
1393 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbvI BcnI
BfaI BmyI BpmI BsaAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I