Restriction map for pF1KB5789
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AgeI        AhdI        AscI        AseI        
AsnI        Asp700I     AspEI       AvrII       BbsI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstZ17I     ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      EcoT22I     FseI        HincII      HindII      
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SspBI       SspI        
SwaI        Tru9I       XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        412  GGTACC               G^GTACC
Aor51HI      1939  AGCGCT               AGC^GCT
Asp718I       412  GGTACC               G^GTACC
AspI         2424  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2312  TGCGCA               TGC^GCA
BamHI         213  GGATCC               G^GATCC
BbvCI         570  CCTCAGC              CCTCAGC(-5/-2)
BcgI          939  CGAGGACTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1917  GGATAC               GTATCC(6/5)
BclI         1329  TGATCA               T^GATCA
BlpI          402  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      402  GCTGAGC              GC^T[ACGT]AGC
BseRI         259  GAGGAG               GAGGAG(10/8)
BsmI          846  GAATGC               GAATGC(1/-1)
BspHI        1575  TCATGA               T^CATGA
BspMI         905  ACCTGC               ACCTGC(4/8)
BsrFI        1735  GCCGGC               [GA]^CCGG[CT]
CelII         402  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1735  GCCGGC               [GA]^CCGG[CT]
DraIII       1114  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          436  CTCTTC               CTCTTC(1/4)
Eco47III     1939  AGCGCT               AGC^GCT
EcoRI         927  GAATTC               G^AATTC
EcoRV        1321  GATATC               GAT^ATC
FbaI         1329  TGATCA               T^GATCA
FspI         2312  TGCGCA               TGC^GCA
HgaI         1200  GACGC                GACGC(5/10)
KpnI          412  GGTACC               GGTAC^C
Ksp632I       436  CTCTTC               CTCTTC(1/4)
NaeI         1735  GCCGGC               GCC^GGC
NgoMIV       1735  GCCGGC               G^CCGGC
PaeR7I       1435  CTCGAG               C^TCGAG
PflFI        2424  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         1575  TCATGA               T^CATGA
SbfI         1866  CCTGCAGG             CCTGCA^GG
Sse8387I     1866  CCTGCAGG             CCTGCA^GG
Tth111I      2424  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1435  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI         1873  GGCGCC               G[GA]^CG[CT]C
             2147  GGCGCC              
ApaI          198  GGGCCC               GGGCC^C
              245  GGGCCC              
BbeI         1873  GGCGCC               GGCGC^C
             2147  GGCGCC              
BsaHI        1873  GGCGCC               G[GA]^CG[CT]C
             2147  GGCGCC              
BspLU11I      309  ACATGT               A^CATGT
              533  ACATGT              
BstAPI       1078  GCAGAGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2304  GCAGTGCATGC         
BstYI         213  GGATCC               [GA]^GATC[CT]
              483  GGATCT              
DrdI         1018  GACCCCACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1065  GACTAGACTGTC        
Hin1I        1873  GGCGCC               G[GA]^CG[CT]C
             2147  GGCGCC              
KasI         1873  GGCGCC               G^GCGCC
             2147  GGCGCC              
MflI          213  GGATCC               [GA]^GATC[CT]
              483  GGATCT              
MslI          708  CACCTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              784  CACTGGAGTG          
NarI         1873  GGCGCC               GG^CGCC
             2147  GGCGCC              
PstI           65  CTGCAG               CTGCA^G
             1867  CTGCAG              
PvuII         541  CAGCTG               CAG^CTG
              622  CAGCTG              
SacI          395  GAGCTC               GAGCT^C
             1039  GAGCTC              
SexAI         362  ACCAGGT              A^CC[AT]GGT
             1050  ACCAGGT             
SfoI         1873  GGCGCC               GGC^GCC
             2147  GGCGCC              
SmaI          828  CCCGGG               CCC^GGG
             1393  CCCGGG              
SphI         2309  GCATGC               GCATG^C
             2336  GCATGC              
StuI          418  AGGCCT               AGG^CCT
              656  AGGCCT              
XcmI          674  CCACTGCCACCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2154  CCAGACTCTCAATGG     
XhoII         213  GGATCC               [GA]^GATC[CT]
              483  GGATCT              
XmaCI         828  CCCGGG               C^CCGGG
             1393  CCCGGG              
XmaI          828  CCCGGG               C^CCGGG
             1393  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbrPI       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaAI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstEII      BstF5I      BstNI       BstPI       BstXI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PmaCI       PmlI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      
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