################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp700I AspEI AvrII BbsI BfrI BglI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoT22I FseI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MseI MunI MvnI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tru9I XbaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 412 GGTACC G^GTACC Aor51HI 1939 AGCGCT AGC^GCT Asp718I 412 GGTACC G^GTACC AspI 2424 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2312 TGCGCA TGC^GCA BamHI 213 GGATCC G^GATCC BbvCI 570 CCTCAGC CCTCAGC(-5/-2) BcgI 939 CGAGGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1917 GGATAC GTATCC(6/5) BclI 1329 TGATCA T^GATCA BlpI 402 GCTGAGC GC^T[ACGT]AGC Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC BseRI 259 GAGGAG GAGGAG(10/8) BsmI 846 GAATGC GAATGC(1/-1) BspHI 1575 TCATGA T^CATGA BspMI 905 ACCTGC ACCTGC(4/8) BsrFI 1735 GCCGGC [GA]^CCGG[CT] CelII 402 GCTGAGC GC^T[ACGT]AGC Cfr10I 1735 GCCGGC [GA]^CCGG[CT] DraIII 1114 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 436 CTCTTC CTCTTC(1/4) Eco47III 1939 AGCGCT AGC^GCT EcoRI 927 GAATTC G^AATTC EcoRV 1321 GATATC GAT^ATC FbaI 1329 TGATCA T^GATCA FspI 2312 TGCGCA TGC^GCA HgaI 1200 GACGC GACGC(5/10) KpnI 412 GGTACC GGTAC^C Ksp632I 436 CTCTTC CTCTTC(1/4) NaeI 1735 GCCGGC GCC^GGC NgoMIV 1735 GCCGGC G^CCGGC PaeR7I 1435 CTCGAG C^TCGAG PflFI 2424 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1575 TCATGA T^CATGA SbfI 1866 CCTGCAGG CCTGCA^GG Sse8387I 1866 CCTGCAGG CCTGCA^GG Tth111I 2424 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1435 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 1873 GGCGCC G[GA]^CG[CT]C 2147 GGCGCC ApaI 198 GGGCCC GGGCC^C 245 GGGCCC BbeI 1873 GGCGCC GGCGC^C 2147 GGCGCC BsaHI 1873 GGCGCC G[GA]^CG[CT]C 2147 GGCGCC BspLU11I 309 ACATGT A^CATGT 533 ACATGT BstAPI 1078 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2304 GCAGTGCATGC BstYI 213 GGATCC [GA]^GATC[CT] 483 GGATCT DrdI 1018 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1065 GACTAGACTGTC Hin1I 1873 GGCGCC G[GA]^CG[CT]C 2147 GGCGCC KasI 1873 GGCGCC G^GCGCC 2147 GGCGCC MflI 213 GGATCC [GA]^GATC[CT] 483 GGATCT MslI 708 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 784 CACTGGAGTG NarI 1873 GGCGCC GG^CGCC 2147 GGCGCC PstI 65 CTGCAG CTGCA^G 1867 CTGCAG PvuII 541 CAGCTG CAG^CTG 622 CAGCTG SacI 395 GAGCTC GAGCT^C 1039 GAGCTC SexAI 362 ACCAGGT A^CC[AT]GGT 1050 ACCAGGT SfoI 1873 GGCGCC GGC^GCC 2147 GGCGCC SmaI 828 CCCGGG CCC^GGG 1393 CCCGGG SphI 2309 GCATGC GCATG^C 2336 GCATGC StuI 418 AGGCCT AGG^CCT 656 AGGCCT XcmI 674 CCACTGCCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2154 CCAGACTCTCAATGG XhoII 213 GGATCC [GA]^GATC[CT] 483 GGATCT XmaCI 828 CCCGGG C^CCGGG 1393 CCCGGG XmaI 828 CCCGGG C^CCGGG 1393 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvI BcnI BfaI BmyI BpmI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I