Restriction map for
pF1KB5907
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BamHI BbeI BbsI BcgI
BciVI BclI BcnI BfrI BglII
BpuAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstYI Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI HphI KasI
KpnI KspI MamI MboI MflI
MluI MroI MseI MslI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 144 GTATAC GT^[AC][GT]AC
Alw44I 14 GTGCAC G^TGCAC
AocI 369 CCTGAGG CC^T[ACGT]AGG
ApaLI 14 GTGCAC G^TGCAC
AspHI 14 GTGCAC G[AT]GC[AT]^C
AvrII 955 CCTAGG C^CTAGG
BanII 690 GAGCCC G[GA]GC[CT]^C
BbrPI 852 CACGTG CAC^GTG
BbvCI 1328 CCTCAGC CCTCAGC(-5/-2)
BglI 692 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 955 CCTAGG C^CTAGG
BlpI 103 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 103 GCTCAGC GC^T[ACGT]AGC
BsaWI 727 ACCGGA [AT]^CCGG[AT]
BsiHKAI 14 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1307 TGTACA T^GTACA
BsrBI 379 GAGCGG CCGCTC(-3/-3)
BsrGI 1307 TGTACA T^GTACA
Bst1107I 144 GTATAC GTA^TAC
BstZ17I 144 GTATAC GTA^TAC
Bsu36I 369 CCTGAGG CC^T[ACGT]AGG
CelII 103 GCTCAGC GC^T[ACGT]AGC
CfoI 1013 GCGC GCG^C
DraII 325 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1095 CCACGG C^C[GA][CT]GG
EarI 460 GAAGAG CTCTTC(1/4)
Eco81I 369 CCTGAGG CC^T[ACGT]AGG
EcoO109I 325 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 1012 GGCGCT [GA]GCGC^[CT]
HapII 728 CCGG C^CGG
HhaI 1013 GCGC GCG^C
HinP1I 1013 GCGC G^CGC
HincII 1345 GTCAAC GT[CT]^[GA]AC
HindII 1345 GTCAAC GT[CT]^[GA]AC
HpaII 728 CCGG C^CGG
Ksp632I 460 GAAGAG CTCTTC(1/4)
MaeIII 1483 GTCAC ^GT[ACGT]AC
MfeI 301 CAATTG C^AATTG
MspI 728 CCGG C^CGG
MunI 301 CAATTG C^AATTG
PmaCI 852 CACGTG CAC^GTG
PmlI 852 CACGTG CAC^GTG
SpeI 741 ACTAGT A^CTAGT
SspBI 1307 TGTACA T^GTACA
StuI 1158 AGGCCT AGG^CCT
Tsp45I 1483 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 615 GAATTT [GA]^AATT[CT]
677 GAATTT
AlwNI 55 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
637 CAGGAACTG
ApoI 615 GAATTT [GA]^AATT[CT]
677 GAATTT
AvaII 312 GGACC G^G[AT]CC
384 GGACC
BalI 158 TGGCCA TGG^CCA
497 TGGCCA
BfaI 742 CTAG C^TAG
956 CTAG
BsaAI 609 TACGTG [CT]AC^GT[GA]
852 CACGTG
BseRI 292 CTCCTC GAGGAG(10/8)
433 CTCCTC
BstXI 404 CCAGAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
500 CCAGGAGGTTGG
EcoT14I 308 CCTTGG C^C[AT][AT]GG
955 CCTAGG
FauI 375 GCGGG CCCGC(4/6)
381 GCGGG
MaeI 742 CTAG C^TAG
956 CTAG
MluNI 158 TGGCCA TGG^CCA
497 TGGCCA
MscI 158 TGGCCA TGG^CCA
497 TGGCCA
PleI 272 GACTC GAGTC(4/5)
1018 GAGTC
PstI 24 CTGCAG CTGCA^G
349 CTGCAG
StyI 308 CCTTGG C^C[AT][AT]GG
955 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaI BanI
BbvI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeII MboII MnlI MspA1I MvaI
MwoI NlaIII NlaIV PvuII RsaI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI Tsp509I TspRI TthHB8I