Restriction map for
pF1KE1394
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MluI MroI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 453 CGCG CG^CG
AflII 516 CTTAAG C^TTAAG
AlwNI 668 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1139 GAGCAC G[AT]GC[AT]^C
AvaII 173 GGACC G^G[AT]CC
BalI 425 TGGCCA TGG^CCA
BfrI 516 CTTAAG C^TTAAG
BglI 241 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 484 AGATCT A^GATCT
BmyI 1139 GAGCAC G[AGT]GC[ACT]^C
BsaAI 682 TACGTG [CT]AC^GT[GA]
BsaBI 192 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1139 GAGCAC G[AT]GC[AT]^C
Bsp1286I 1139 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 1292 TGTACA T^GTACA
BspHI 752 TCATGA T^CATGA
BsrFI 1035 GCCGGT [GA]^CCGG[CT]
BsrGI 1292 TGTACA T^GTACA
BstUI 453 CGCG CG^CG
BstXI 737 CCATTGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1035 GCCGGT [GA]^CCGG[CT]
Cfr13I 173 GGACC G^G[ACGT]CC
EaeI 425 TGGCCA [CT]^GGCC[GA]
EcoNI 313 CCTTATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 46 GAATTC G^AATTC
EcoT22I 97 ATGCAT ATGCA^T
HapII 1036 CCGG C^CGG
HincII 955 GTTGAC GT[CT]^[GA]AC
HindII 955 GTTGAC GT[CT]^[GA]AC
HpaII 1036 CCGG C^CGG
MamI 192 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 569 CAATTG C^AATTG
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MspA1I 1107 CAGCGG C[AC]G^C[GT]G
MspI 1036 CCGG C^CGG
MunI 569 CAATTG C^AATTG
MvnI 453 CGCG CG^CG
NsiI 97 ATGCAT ATGCA^T
NspI 99 GCATGT [GA]CATG^[CT]
RcaI 752 TCATGA T^CATGA
SapI 1154 GAAGAGC GCTCTTC(1/4)
Sau96I 173 GGACC G^G[ACGT]CC
SmlI 516 CTTAAG C^T[CT][GA]AG
SspBI 1292 TGTACA T^GTACA
SspI 832 AATATT AAT^ATT
Tsp45I 1521 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 151 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1045 GAAGTGGTTC
BamHI 197 GGATCC G^GATCC
717 GGATCC
BbsI 606 GTCTTC GAAGAC(2/6)
1017 GTCTTC
BpmI 182 CTCCAG CTGGAG(16/14)
1234 CTGGAG
BpuAI 606 GTCTTC GAAGAC(2/6)
1017 GTCTTC
BsiYI 313 CCTTATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1144 CCTGCAAAGGG
BslI 313 CCTTATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1144 CCTGCAAAGGG
BspMI 663 ACCTGC ACCTGC(4/8)
1143 ACCTGC
DdeI 119 CTCAG C^T[ACGT]AG
602 CTCAG
DsaI 428 CCATGG C^C[GA][CT]GG
965 CCATGG
EcoT14I 428 CCATGG C^C[AT][AT]GG
965 CCATGG
HphI 459 GGTGA GGTGA(8/7)
928 TCACC
MaeII 683 ACGT A^CGT
920 ACGT
NcoI 428 CCATGG C^CATGG
965 CCATGG
SfaNI 96 GATGC GCATC(5/9)
548 GCATC
StuI 10 AGGCCT AGG^CCT
37 AGGCCT
StyI 428 CCATGG C^C[AT][AT]GG
965 CCATGG
TspRI 474 CAGTG CA[GC]TG[ACGT][ACGT]^
1311 CACTG
XmnI 151 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1045 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsaJI BseRI BsmAI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI ScrFI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I XhoII