Restriction map for
pF1KB9925
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HindIII HpaI HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TfiI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 908 ACATGT A^C[GA][CT]GT
AlwNI 773 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 372 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 136 GGACC G^G[AT]CC
BanII 844 GAGCTC G[GA]GC[CT]^C
BbsI 567 GAAGAC GAAGAC(2/6)
BcgI 735 CGAAGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 423 TGATCA T^GATCA
BfaI 125 CTAG C^TAG
BpuAI 567 GAAGAC GAAGAC(2/6)
BsaI 484 GGTCTC GGTCTC(1/5)
BsmI 745 GCATTC GAATGC(1/-1)
BspHI 245 TCATGA T^CATGA
BspLU11I 908 ACATGT A^CATGT
BsrDI 864 CATTGC GCAATG(2/0)
BstAPI 447 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 447 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 720 GCGC GCG^C
EarI 959 GAAGAG CTCTTC(1/4)
EcoRV 304 GATATC GAT^ATC
FbaI 423 TGATCA T^GATCA
HhaI 720 GCGC GCG^C
HinP1I 720 GCGC G^CGC
HincII 825 GTTGAC GT[CT]^[GA]AC
HindII 825 GTTGAC GT[CT]^[GA]AC
Ksp632I 959 GAAGAG CTCTTC(1/4)
MaeI 125 CTAG C^TAG
MaeIII 508 GTTAC ^GT[ACGT]AC
NspI 908 ACATGT [GA]CATG^[CT]
RcaI 245 TCATGA T^CATGA
SacI 844 GAGCTC GAGCT^C
SmaI 11 CCCGGG CCC^GGG
SmlI 5 CTCAAG C^T[CT][GA]AG
TaqI 734 TCGA T^CGA
TthHB8I 734 TCGA T^CGA
XmaCI 11 CCCGGG C^CCGGG
XmaI 11 CCCGGG C^CCGGG
XmnI 372 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 543 AAATTT [GA]^AATT[CT]
677 AAATTC
AfaI 99 GTAC GT^AC
855 GTAC
ApoI 543 AAATTT [GA]^AATT[CT]
677 AAATTC
AspHI 844 GAGCTC G[AT]GC[AT]^C
1029 GAGCAC
AvaI 11 CCCGGG C^[CT]CG[GA]G
755 CCCGAG
BglII 178 AGATCT A^GATCT
881 AGATCT
BpmI 841 CTGGAG CTGGAG(16/14)
1001 CTGGAG
BsiHKAI 844 GAGCTC G[AT]GC[AT]^C
1029 GAGCAC
BsiYI 12 CCGGGTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
975 CCCCTCTAAGG
BslI 12 CCGGGTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
975 CCCCTCTAAGG
BsmAI 622 GAGAC GTCTC(1/5)
485 GTCTC
BsoBI 11 CCCGGG C^[CT]CG[GA]G
755 CCCGAG
BstYI 178 AGATCT [GA]^GATC[CT]
881 AGATCT
HapII 12 CCGG C^CGG
832 CCGG
HinfI 3 GACTC G^A[ACGT]TC
919 GACTC
HpaII 12 CCGG C^CGG
832 CCGG
MaeII 172 ACGT A^CGT
1010 ACGT
MflI 178 AGATCT [GA]^GATC[CT]
881 AGATCT
MslI 796 CACAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
909 CATGTTCGTG
MspI 12 CCGG C^CGG
832 CCGG
NlaIV 285 GGCTCC GG[ACGT]^[ACGT]CC
477 GGCCCC
PleI 3 GACTC GAGTC(4/5)
919 GACTC
RsaI 99 GTAC GT^AC
855 GTAC
SfaNI 739 GATGC GCATC(5/9)
775 GATGC
SspI 782 AATATT AAT^ATT
938 AATATT
XhoII 178 AGATCT [GA]^GATC[CT]
881 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI BbvI BcnI BmyI BsaJI
BseRI BsgI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII ItaI
MboI MboII MnlI MseI MvaI
MwoI NciI NdeII NlaIII PstI
Sau3AI Sau96I ScrFI SfcI Tru9I
TseI Tsp509I TspRI