Restriction map for
pF1KB8730
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1125 GTCTAC GT^[AC][GT]AC
AclI 88 AACGTT AA^CGTT
AcsI 597 AAATTT [GA]^AATT[CT]
AflIII 707 ACATGT A^C[GA][CT]GT
AocI 667 CCTGAGG CC^T[ACGT]AGG
ApoI 597 AAATTT [GA]^AATT[CT]
AvaII 175 GGTCC G^G[AT]CC
BbvCI 571 CCTCAGC CCTCAGC(-5/-2)
BfaI 23 CTAG C^TAG
BsaAI 372 CACGTA [CT]AC^GT[GA]
BsaI 839 GAGACC GGTCTC(1/5)
BsaWI 740 ACCGGA [AT]^CCGG[AT]
BsmAI 839 GAGAC GTCTC(1/5)
BspLU11I 707 ACATGT A^CATGT
BspMI 418 ACCTGC ACCTGC(4/8)
BsrDI 115 GCAATG GCAATG(2/0)
BssSI 889 CACGAG CACGAG(-5/-1)
Bsu36I 667 CCTGAGG CC^T[ACGT]AGG
Cac8I 915 GCGAGC GC[ACGT]^[ACGT]GC
DraII 1164 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 351 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 856 GAAGAG CTCTTC(1/4)
Eco81I 667 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1164 AGGGCCT [GA]G^G[ACGT]CC[CT]
Ksp632I 856 GAAGAG CTCTTC(1/4)
MaeI 23 CTAG C^TAG
MspA1I 430 CAGCTG C[AC]G^C[GT]G
NcoI 476 CCATGG C^CATGG
NspI 707 ACATGT [GA]CATG^[CT]
PshAI 169 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 88 AACGTT AA^CGTT
PvuII 430 CAGCTG CAG^CTG
SgrAI 242 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1048 CTCAAG C^T[CT][GA]AG
SspI 977 AATATT AAT^ATT
StuI 1037 AGGCCT AGG^CCT
Tsp509I 598 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 375 GTAC GT^AC
686 GTAC
AlwI 19 GATCC GGATC(4/5)
993 GATCC
AlwNI 200 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
717 CAGTTCCTG
Aor51HI 539 AGCGCT AGC^GCT
1007 AGCGCT
AspHI 906 GAGCAC G[AT]GC[AT]^C
917 GAGCAC
BalI 410 TGGCCA TGG^CCA
479 TGGCCA
BanI 460 GGCACC G^G[CT][GA]CC
651 GGTGCC
BanII 31 GAGCCC G[GA]GC[CT]^C
1088 GGGCTC
BbsI 166 GAAGAC GAAGAC(2/6)
772 GAAGAC
BcnI 138 CCCGG CC^[GC]GG
457 CCCGG
BpmI 777 CTCCAG CTGGAG(16/14)
1095 CTCCAG
BpuAI 166 GAAGAC GAAGAC(2/6)
772 GAAGAC
BsiHKAI 906 GAGCAC G[AT]GC[AT]^C
917 GAGCAC
BsmI 194 GAATGC GAATGC(1/-1)
342 GCATTC
BsrFI 243 ACCGGC [GA]^CCGG[CT]
842 ACCGGC
BstYI 18 AGATCC [GA]^GATC[CT]
992 AGATCC
Cfr10I 243 ACCGGC [GA]^CCGG[CT]
842 ACCGGC
DpnII 19 GATC ^GATC
993 GATC
DsaI 476 CCATGG C^C[GA][CT]GG
996 CCGTGG
EaeI 410 TGGCCA [CT]^GGCC[GA]
479 TGGCCA
Eco47III 539 AGCGCT AGC^GCT
1007 AGCGCT
EcoT14I 476 CCATGG C^C[AT][AT]GG
1040 CCTTGG
HgaI 26 GCGTC GACGC(5/10)
467 GCGTC
MaeII 89 ACGT A^CGT
373 ACGT
MboI 19 GATC ^GATC
993 GATC
MflI 18 AGATCC [GA]^GATC[CT]
992 AGATCC
MluNI 410 TGGCCA TGG^CCA
479 TGGCCA
MscI 410 TGGCCA TGG^CCA
479 TGGCCA
NciI 138 CCCGG CC^[GC]GG
457 CCCGG
NdeII 19 GATC ^GATC
993 GATC
PflMI 111 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCACTGGATGG
RsaI 375 GTAC GT^AC
686 GTAC
Sau3AI 19 GATC ^GATC
993 GATC
StyI 476 CCATGG C^C[AT][AT]GG
1040 CCTTGG
TfiI 442 GATTC G^A[AT]TC
861 GAATC
Tsp45I 364 GTGAC ^GT[GC]AC
495 GTCAC
Van91I 111 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCACTGGATGG
XcmI 259 CCAGCCCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
758 CCATCATCACACTGG
XhoII 18 AGATCC [GA]^GATC[CT]
992 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI CfoI Cfr13I DdeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboII
MnlI MspI MvaI MvnI MwoI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I