Restriction map for
pF1KE0668
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HphI KasI KpnI Ksp632I KspI
MaeII MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 320 TCCGGA T^CCGGA
AfaI 372 GTAC GT^AC
AflIII 686 ACATGT A^C[GA][CT]GT
AlwI 453 GGATC GGATC(4/5)
AspHI 430 GAGCAC G[AT]GC[AT]^C
AvaI 877 CCCGGG C^[CT]CG[GA]G
BanI 827 GGTGCC G^G[CT][GA]CC
BbvCI 1096 GCTGAGG CCTCAGC(-5/-2)
BcgI 45 CGACGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 780 GTATCC GTATCC(6/5)
BglII 1087 AGATCT A^GATCT
BsaBI 414 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 320 TCCGGA [AT]^CCGG[AT]
BseAI 320 TCCGGA T^CCGGA
BsgI 724 GTGCAG GTGCAG(16/14)
BsiHKAI 430 GAGCAC G[AT]GC[AT]^C
BsoBI 877 CCCGGG C^[CT]CG[GA]G
BspEI 320 TCCGGA T^CCGGA
BspLU11I 686 ACATGT A^CATGT
BsrI 977 CCAGT ACTGG(1/-1)
BstAPI 345 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1087 AGATCT [GA]^GATC[CT]
CfoI 144 GCGC GCG^C
DrdI 622 GACTTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 479 GAATTC G^AATTC
HaeII 143 AGCGCC [GA]GCGC^[CT]
HhaI 144 GCGC GCG^C
HinP1I 144 GCGC G^CGC
HpaI 58 GTTAAC GTT^AAC
MaeIII 620 GTGAC ^GT[ACGT]AC
MamI 414 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1087 AGATCT [GA]^GATC[CT]
MroI 320 TCCGGA T^CCGGA
NspI 686 ACATGT [GA]CATG^[CT]
PvuII 28 CAGCTG CAG^CTG
RsaI 372 GTAC GT^AC
SmaI 877 CCCGGG CCC^GGG
SmlI 148 CTTGAG C^T[CT][GA]AG
TaqI 647 TCGA T^CGA
Tsp45I 620 GTGAC ^GT[GC]AC
TthHB8I 647 TCGA T^CGA
XhoII 1087 AGATCT [GA]^GATC[CT]
XmaCI 877 CCCGGG C^CCGGG
XmaI 877 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 18 GACGCC G[GA]^CG[CT]C
1174 GGCGTC
AlwNI 346 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
417 CAGTATCTG
BalI 281 TGGCCA TGG^CCA
656 TGGCCA
BfaI 118 CTAG C^TAG
589 CTAG
BmyI 430 GAGCAC G[AGT]GC[ACT]^C
672 GGGCAC
BsaHI 18 GACGCC G[GA]^CG[CT]C
1174 GGCGTC
Bsp1286I 430 GAGCAC G[AGT]GC[ACT]^C
672 GGGCAC
BspMI 345 GCAGGT ACCTGC(4/8)
1114 GCAGGT
BsrDI 510 CATTGC GCAATG(2/0)
230 GCAATG
BstXI 803 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1164 CCATGCTGCTGG
EaeI 281 TGGCCA [CT]^GGCC[GA]
656 TGGCCA
HgaI 18 GACGC GACGC(5/10)
1175 GCGTC
Hin1I 18 GACGCC G[GA]^CG[CT]C
1174 GGCGTC
MaeI 118 CTAG C^TAG
589 CTAG
MluNI 281 TGGCCA TGG^CCA
656 TGGCCA
MscI 281 TGGCCA TGG^CCA
656 TGGCCA
MslI 433 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
562 CACAGAGATG
MspA1I 28 CAGCTG C[AC]G^C[GT]G
633 CAGCGG
NcoI 653 CCATGG C^CATGG
920 CCATGG
PpuMI 679 AGGTCCT [GA]G^G[AT]CC[CT]
871 AGGACCC
PstI 53 CTGCAG CTGCA^G
343 CTGCAG
TspRI 213 CAGTG CA[GC]TG[ACGT][ACGT]^
978 CAGTG
XcmI 258 CCACAAGCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
659 CCAACTCCTTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BcnI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII ItaI MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI Tru9I TseI Tsp509I