Restriction map for
pF1KB5427
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsiEI BsiWI BspDI
BspHI BspLU11I BsrDI BsrFI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 137 TCCGGA T^CCGGA
AcyI 888 GACGCC G[GA]^CG[CT]C
AocI 68 CCTCAGG CC^T[ACGT]AGG
BamHI 770 GGATCC G^GATCC
BclI 986 TGATCA T^GATCA
BglI 900 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 888 GACGCC G[GA]^CG[CT]C
BsaWI 137 TCCGGA [AT]^CCGG[AT]
BseAI 137 TCCGGA T^CCGGA
BsgI 820 CTGCAC GTGCAG(16/14)
BsmBI 886 GAGACG CGTCTC(1/5)
BsmI 214 GAATGC GAATGC(1/-1)
Bsp1407I 682 TGTACA T^GTACA
BspEI 137 TCCGGA T^CCGGA
BspMI 567 GCAGGT ACCTGC(4/8)
BsrBI 993 GAGCGG CCGCTC(-3/-3)
BsrGI 682 TGTACA T^GTACA
BssHII 237 GCGCGC G^CGCGC
BssSI 922 CTCGTG CACGAG(-5/-1)
BstAPI 209 GCACGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 353 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 770 GGATCC [GA]^GATC[CT]
Bsu36I 68 CCTCAGG CC^T[ACGT]AGG
DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 893 CCATGG C^C[GA][CT]GG
Eco81I 68 CCTCAGG CC^T[ACGT]AGG
EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 986 TGATCA T^GATCA
Hin1I 888 GACGCC G[GA]^CG[CT]C
MaeII 56 ACGT A^CGT
MaeIII 1072 GTGAC ^GT[ACGT]AC
MflI 770 GGATCC [GA]^GATC[CT]
MroI 137 TCCGGA T^CCGGA
MseI 122 TTAA T^TAA
NcoI 893 CCATGG C^CATGG
NspI 563 GCATGC [GA]CATG^[CT]
PflMI 969 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 725 GAGTC GAGTC(4/5)
PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 150 CTGCAG CTGCA^G
ScaI 860 AGTACT AGT^ACT
SexAI 41 ACCTGGT A^CC[AT]GGT
SmaI 575 CCCGGG CCC^GGG
SphI 563 GCATGC GCATG^C
SspBI 682 TGTACA T^GTACA
TaqI 1056 TCGA T^CGA
TfiI 516 GATTC G^A[AT]TC
Tru9I 122 TTAA T^TAA
Tsp45I 1072 GTGAC ^GT[GC]AC
TthHB8I 1056 TCGA T^CGA
Van91I 969 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 770 GGATCC [GA]^GATC[CT]
XmaCI 575 CCCGGG C^CCGGG
XmaI 575 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 807 GTGCTC G[AT]GC[AT]^C
952 GTGCTC
AvaI 496 CCCGAG C^[CT]CG[GA]G
575 CCCGGG
BalI 362 TGGCCA TGG^CCA
1013 TGGCCA
BanI 874 GGCACC G^G[CT][GA]CC
897 GGTGCC
BanII 348 GGGCTC G[GA]GC[CT]^C
1089 GGGCTC
BpmI 193 CTGGAG CTGGAG(16/14)
315 CTGGAG
BsiHKAI 807 GTGCTC G[AT]GC[AT]^C
952 GTGCTC
BsmAI 886 GAGAC GTCTC(1/5)
24 GTCTC
BsoBI 496 CCCGAG C^[CT]CG[GA]G
575 CCCGGG
DdeI 26 CTCAG C^T[ACGT]AG
69 CTCAG
EaeI 362 TGGCCA [CT]^GGCC[GA]
1013 TGGCCA
EarI 97 CTCTTC CTCTTC(1/4)
133 CTCTTC
HgaI 888 GACGC GACGC(5/10)
1008 GACGC
HinfI 516 GATTC G^A[ACGT]TC
725 GAGTC
Ksp632I 97 CTCTTC CTCTTC(1/4)
133 CTCTTC
MluNI 362 TGGCCA TGG^CCA
1013 TGGCCA
MscI 362 TGGCCA TGG^CCA
1013 TGGCCA
MspA1I 178 CAGCTG C[AC]G^C[GT]G
668 CAGCTG
PvuII 178 CAGCTG CAG^CTG
668 CAGCTG
SapI 96 GCTCTTC GCTCTTC(1/4)
132 GCTCTTC
SfcI 150 CTGCAG C^T[GA][CT]AG
864 CTACAG
XcmI 365 CCAAGAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
388 CCAATTATCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BbsI BbvI BcnI
BmyI BpuAI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TseI Tsp509I
TspRI