Restriction map for
pF1KE1005
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeIII
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 226 GTAGAC GT^[AC][GT]AC
AcsI 496 GAATTC [GA]^AATT[CT]
AluI 482 AGCT AG^CT
ApoI 496 GAATTC [GA]^AATT[CT]
AvaII 306 GGACC G^G[AT]CC
BalI 537 TGGCCA TGG^CCA
BamHI 245 GGATCC G^GATCC
BanI 70 GGCACC G^G[CT][GA]CC
BfaI 418 CTAG C^TAG
BpmI 436 CTCCAG CTGGAG(16/14)
BsaAI 529 TACGTG [CT]AC^GT[GA]
BsaWI 477 ACCGGA [AT]^CCGG[AT]
BspMI 346 ACCTGC ACCTGC(4/8)
BsrI 250 CCAGT ACTGG(1/-1)
BstF5I 534 GGATG GGATG(2/0)
DdeI 234 CTCAG C^T[ACGT]AG
DraII 316 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoNI 432 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 316 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 496 GAATTC G^AATTC
EcoT14I 20 CCATGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
FokI 534 GGATG GGATG(9/13)
HgaI 178 GACGC GACGC(5/10)
HinfI 263 GAGTC G^A[ACGT]TC
MaeI 418 CTAG C^TAG
MluNI 537 TGGCCA TGG^CCA
MscI 537 TGGCCA TGG^CCA
MslI 352 CATGTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 241 CAGCGG C[AC]G^C[GT]G
NcoI 20 CCATGG C^CATGG
NsiI 1 ATGCAT ATGCA^T
PleI 263 GAGTC GAGTC(4/5)
PstI 55 CTGCAG CTGCA^G
SexAI 73 ACCTGGT A^CC[AT]GGT
SfcI 55 CTGCAG C^T[GA][CT]AG
StyI 20 CCATGG C^C[AT][AT]GG
Tsp509I 497 AATT ^AATT
TspRI 251 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbsI 367 GAAGAC GAAGAC(2/6)
406 GAAGAC
BglI 16 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
328 GCCTCCCTGGC
BmyI 69 GGGCAC G[AGT]GC[ACT]^C
93 GGGCAC
BpuAI 367 GAAGAC GAAGAC(2/6)
406 GAAGAC
Bsp1286I 69 GGGCAC G[AGT]GC[ACT]^C
93 GGGCAC
BstYI 245 GGATCC [GA]^GATC[CT]
378 GGATCT
DraIII 72 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
298 CACGGGGTG
DsaI 20 CCATGG C^C[GA][CT]GG
297 CCACGG
EaeI 11 TGGCCG [CT]^GGCC[GA]
537 TGGCCA
FauI 166 GCGGG CCCGC(4/6)
181 GCGGG
HaeII 120 GGCGCT [GA]GCGC^[CT]
293 AGCGCC
MflI 245 GGATCC [GA]^GATC[CT]
378 GGATCT
NspI 3 GCATGT [GA]CATG^[CT]
413 ACATGC
PflMI 250 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
297 CCACGGGGTGG
SmaI 26 CCCGGG CCC^GGG
199 CCCGGG
Van91I 250 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
297 CCACGGGGTGG
XhoII 245 GGATCC [GA]^GATC[CT]
378 GGATCT
XmaCI 26 CCCGGG C^CCGGG
199 CCCGGG
XmaI 26 CCCGGG C^CCGGG
199 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AlwI AvaI BbvI
BcnI BsaJI BseRI BsiYI BslI
BsmFI BsoBI BsrBI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DpnII
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI