Restriction map for
pF1KE1020
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccII AccIII AclI AflII
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BlnI
BpuAI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BstBI BstUI BstXI Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FbaI FspI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1951 GGTACC G^GTACC
AcsI 718 GAATTT [GA]^AATT[CT]
AflIII 962 ACGTGT A^C[GA][CT]GT
AgeI 1268 ACCGGT A^CCGGT
AhdI 973 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 718 GAATTT [GA]^AATT[CT]
Asp718I 1951 GGTACC G^GTACC
AspEI 973 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1744 TGGCCA TGG^CCA
BglII 1893 AGATCT A^GATCT
BlpI 321 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 321 GCTTAGC GC^T[ACGT]AGC
BsaI 980 GGTCTC GGTCTC(1/5)
BsaWI 1268 ACCGGT [AT]^CCGG[AT]
BsmBI 1921 CGTCTC CGTCTC(1/5)
BsmI 354 GAATGC GAATGC(1/-1)
Bsp1407I 562 TGTACA T^GTACA
BsrGI 562 TGTACA T^GTACA
Bst1107I 762 GTATAC GTA^TAC
BstAPI 93 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1893 AGATCT [GA]^GATC[CT]
BstZ17I 762 GTATAC GTA^TAC
CelII 321 GCTTAGC GC^T[ACGT]AGC
DraIII 1235 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 973 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1779 GATATC GAT^ATC
FseI 1852 GGCCGGCC GGCCGG^CC
KpnI 1951 GGTACC GGTAC^C
MflI 1893 AGATCT [GA]^GATC[CT]
MluNI 1744 TGGCCA TGG^CCA
MscI 1744 TGGCCA TGG^CCA
PinAI 1268 ACCGGT A^CCGGT
SmaI 1331 CCCGGG CCC^GGG
SspBI 562 TGTACA T^GTACA
TaqI 1157 TCGA T^CGA
Tsp509I 719 AATT ^AATT
TthHB8I 1157 TCGA T^CGA
XcmI 701 CCATGCTGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1893 AGATCT [GA]^GATC[CT]
XmaCI 1331 CCCGGG C^CCGGG
XmaI 1331 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 762 GTATAC GT^[AC][GT]AC
958 GTAGAC
BbeI 1178 GGCGCC GGCGC^C
1661 GGCGCC
BsaAI 798 CACGTA [CT]AC^GT[GA]
1637 TACGTG
BsgI 666 CTGCAC GTGCAG(16/14)
1706 CTGCAC
BstEII 144 GGTCACC G^GT[ACGT]ACC
1003 GGTGACC
BstPI 144 GGTCACC G^GT[ACGT]ACC
1003 GGTGACC
DpnII 282 GATC ^GATC
1894 GATC
EcoO65I 144 GGTCACC G^GT[ACGT]ACC
1003 GGTGACC
EcoT14I 812 CCATGG C^C[AT][AT]GG
968 CCATGG
HaeII 1178 GGCGCC [GA]GCGC^[CT]
1661 GGCGCC
HgaI 527 GCGTC GACGC(5/10)
1920 GCGTC
KasI 1178 GGCGCC G^GCGCC
1661 GGCGCC
MboI 282 GATC ^GATC
1894 GATC
MslI 135 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
273 CACTCTAGTG
NarI 1178 GGCGCC GG^CGCC
1661 GGCGCC
NcoI 812 CCATGG C^CATGG
968 CCATGG
NdeII 282 GATC ^GATC
1894 GATC
PflMI 1266 CCACCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1716 CCACTCCCTGG
SacI 1814 GAGCTC GAGCT^C
1899 GAGCTC
SapI 519 GCTCTTC GCTCTTC(1/4)
1907 GCTCTTC
Sau3AI 282 GATC ^GATC
1894 GATC
SfiI 1141 GGCCTGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1852 GGCCGGCCTGGCC
SfoI 1178 GGCGCC GGC^GCC
1661 GGCGCC
SmlI 508 CTCAAG C^T[CT][GA]AG
1540 CTTGAG
StyI 812 CCATGG C^C[AT][AT]GG
968 CCATGG
Van91I 1266 CCACCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1716 CCACTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NaeI
NciI NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI