Restriction map for
pF1KB9485
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AflII AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BcnI BfrI
BlnI BlpI Bpu1102I BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BsrBI BsrGI BssHII Bst1107I BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MluI NaeI NarI NciI NdeI
NgoMIV NheI NotI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SfiI
SfoI SgrAI SmaI SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 637 CGCG CG^CG
AccIII 195 TCCGGA T^CCGGA
AclI 1277 AACGTT AA^CGTT
AflIII 4087 ACATGT A^C[GA][CT]GT
AgeI 1470 ACCGGT A^CCGGT
Alw44I 2804 GTGCAC G^TGCAC
ApaLI 2804 GTGCAC G^TGCAC
BalI 2 TGGCCA TGG^CCA
BciVI 4405 GTATCC GTATCC(6/5)
BsaAI 3091 TACGTA [CT]AC^GT[GA]
BsaI 2521 GAGACC GGTCTC(1/5)
BseAI 195 TCCGGA T^CCGGA
BspEI 195 TCCGGA T^CCGGA
BspHI 4170 TCATGA T^CATGA
BspLU11I 4087 ACATGT A^CATGT
BssSI 2518 CACGAG CACGAG(-5/-1)
BstEII 2833 GGTTACC G^GT[ACGT]ACC
BstPI 2833 GGTTACC G^GT[ACGT]ACC
BstUI 637 CGCG CG^CG
BstXI 6093 CCATTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 2833 GGTTACC G^GT[ACGT]ACC
EcoRV 5142 GATATC GAT^ATC
FauI 666 GCGGG CCCGC(4/6)
HaeII 710 AGCGCC [GA]GCGC^[CT]
MluNI 2 TGGCCA TGG^CCA
MroI 195 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MvnI 637 CGCG CG^CG
NcoI 3745 CCATGG C^CATGG
NruI 636 TCGCGA TCG^CGA
PflMI 208 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1470 ACCGGT A^CCGGT
Psp1406I 1277 AACGTT AA^CGTT
RcaI 4170 TCATGA T^CATGA
SbfI 4745 CCTGCAGG CCTGCA^GG
ScaI 1435 AGTACT AGT^ACT
SexAI 3525 ACCAGGT A^CC[AT]GGT
SnaBI 3091 TACGTA TAC^GTA
SpeI 5692 ACTAGT A^CTAGT
Sse8387I 4745 CCTGCAGG CCTGCA^GG
Van91I 208 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2793 GTCTAC GT^[AC][GT]AC
4206 GTCTAC
AcyI 1108 GGCGTC G[GA]^CG[CT]C
3866 GACGCC
BanI 103 GGTGCC G^G[CT][GA]CC
1467 GGCACC
BbvCI 910 CCTCAGC CCTCAGC(-5/-2)
2749 CCTCAGC
BclI 39 TGATCA T^GATCA
5421 TGATCA
BglI 3973 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5269 GCCAAAGAGGC
BsaHI 1108 GGCGTC G[GA]^CG[CT]C
3866 GACGCC
BsaWI 195 TCCGGA [AT]^CCGG[AT]
1470 ACCGGT
BsrFI 1470 ACCGGT [GA]^CCGG[CT]
4898 ACCGGC
BstBI 1604 TTCGAA TT^CGAA
1760 TTCGAA
Cfr10I 1470 ACCGGT [GA]^CCGG[CT]
4898 ACCGGC
DraI 5644 TTTAAA TTT^AAA
5763 TTTAAA
DsaI 896 CCACGG C^C[GA][CT]GG
3745 CCATGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
899 CGGCCA
EcoRI 3621 GAATTC G^AATTC
4023 GAATTC
EcoT22I 464 ATGCAT ATGCA^T
4388 ATGCAT
FbaI 39 TGATCA T^GATCA
5421 TGATCA
Hin1I 1108 GGCGTC G[GA]^CG[CT]C
3866 GACGCC
MfeI 2359 CAATTG C^AATTG
2982 CAATTG
MunI 2359 CAATTG C^AATTG
2982 CAATTG
NsiI 464 ATGCAT ATGCA^T
4388 ATGCAT
NspV 1604 TTCGAA TT^CGAA
1760 TTCGAA
SacI 998 GAGCTC GAGCT^C
3082 GAGCTC
SapI 2878 GAAGAGC GCTCTTC(1/4)
4132 GAAGAGC
SfuI 1604 TTCGAA TT^CGAA
1760 TTCGAA
SphI 2422 GCATGC GCATG^C
3047 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaBI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI