Restriction map for
pF1KA0869
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
AclI AgeI Aor51HI ApaI AscI
Asp700I AspI AviII AvrII BbrPI
BcgI BclI BlnI BlpI Bpu1102I
BsiWI BsmBI BsmI Bsp1407I BspHI
BspLU11I BsrDI BsrGI Bst1107I BstEII
BstPI BstZ17I CelII CpoI DraI
DraIII Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI FspI HpaI MluI
NcoI NheI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SapI ScaI SfiI SgrAI
SnaBI SpeI SspBI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 3334 GACGTC GACGT^C
Acc65I 2642 GGTACC G^GTACC
AcsI 1982 GAATTC [GA]^AATT[CT]
AflII 2229 CTTAAG C^TTAAG
AflIII 2813 ACGTGT A^C[GA][CT]GT
ApoI 1982 GAATTC [GA]^AATT[CT]
AseI 2031 ATTAAT AT^TAAT
AsnI 2031 ATTAAT AT^TAAT
Asp718I 2642 GGTACC G^GTACC
BbeI 2284 GGCGCC GGCGC^C
BbvCI 3129 GCTGAGG CCTCAGC(-5/-2)
BciVI 2194 GTATCC GTATCC(6/5)
BfrI 2229 CTTAAG C^TTAAG
BsaAI 2812 TACGTG [CT]AC^GT[GA]
BsiEI 7 CGGCCG CG[GA][CT]^CG
BspDI 592 ATCGAT AT^CGAT
BssHII 43 GCGCGC G^CGCGC
BstBI 2876 TTCGAA TT^CGAA
BstXI 1824 CCAGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 592 ATCGAT AT^CGAT
DrdI 55 GACGGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 7 CGGCCG C^GGCCG
EclXI 7 CGGCCG C^GGCCG
Eco52I 7 CGGCCG C^GGCCG
EcoRI 1982 GAATTC G^AATTC
KasI 2284 GGCGCC G^GCGCC
KpnI 2642 GGTACC GGTAC^C
KspI 109 CCGCGG CCGC^GG
MfeI 3010 CAATTG C^AATTG
MunI 3010 CAATTG C^AATTG
NaeI 2819 GCCGGC GCC^GGC
NarI 2284 GGCGCC GG^CGCC
NgoMIV 2819 GCCGGC G^CCGGC
NspV 2876 TTCGAA TT^CGAA
PaeR7I 251 CTCGAG C^TCGAG
PshBI 2031 ATTAAT AT^TAAT
SacII 109 CCGCGG CCGC^GG
SalI 2328 GTCGAC G^TCGAC
SfoI 2284 GGCGCC GGC^GCC
SfuI 2876 TTCGAA TT^CGAA
SspI 2028 AATATT AAT^ATT
StuI 983 AGGCCT AGG^CCT
XcmI 1235 CCACCCGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 251 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 64 GTCTAC GT^[AC][GT]AC
2328 GTCGAC
AccIII 2138 TCCGGA T^CCGGA
2903 TCCGGA
Alw44I 323 GTGCAC G^TGCAC
3220 GTGCAC
ApaLI 323 GTGCAC G^TGCAC
3220 GTGCAC
BalI 965 TGGCCA TGG^CCA
1517 TGGCCA
BamHI 246 GGATCC G^GATCC
1318 GGATCC
BfaI 169 CTAG C^TAG
2170 CTAG
BglI 307 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2276 GCCTGGGAGGC
BsaBI 1023 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3306 GATGAATATC
BseAI 2138 TCCGGA T^CCGGA
2903 TCCGGA
BsgI 1255 CTGCAC GTGCAG(16/14)
1912 GTGCAG
BspEI 2138 TCCGGA T^CCGGA
2903 TCCGGA
BssSI 547 CACGAG CACGAG(-5/-1)
1504 CACGAG
DsaI 109 CCGCGG C^C[GA][CT]GG
3072 CCACGG
HincII 511 GTCAAC GT[CT]^[GA]AC
2328 GTCGAC
HindII 511 GTCAAC GT[CT]^[GA]AC
2328 GTCGAC
HindIII 864 AAGCTT A^AGCTT
970 AAGCTT
MaeI 169 CTAG C^TAG
2170 CTAG
MamI 1023 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3306 GATGAATATC
MluNI 965 TGGCCA TGG^CCA
1517 TGGCCA
MroI 2138 TCCGGA T^CCGGA
2903 TCCGGA
MscI 965 TGGCCA TGG^CCA
1517 TGGCCA
NdeI 185 CATATG CA^TATG
280 CATATG
NspI 393 GCATGC [GA]CATG^[CT]
1286 GCATGC
PflMI 92 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
193 CCACAGGTTGG
PpuMI 1136 AGGACCT [GA]G^G[AT]CC[CT]
2796 AGGACCC
SexAI 296 ACCTGGT A^CC[AT]GGT
341 ACCAGGT
SmaI 506 CCCGGG CCC^GGG
1151 CCCGGG
SphI 393 GCATGC GCATG^C
1286 GCATGC
TfiI 1957 GAATC G^A[AT]TC
2826 GAATC
Tsp45I 1952 GTGAC ^GT[GC]AC
2840 GTGAC
Van91I 92 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
193 CCACAGGTTGG
XmaCI 506 CCCGGG C^CCGGG
1151 CCCGGG
XmaI 506 CCCGGG C^CCGGG
1151 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI AspEI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI Sse8387I
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII