Restriction map for
pF1KA1662
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AclI AflII AscI AseI
AsnI BbeI BcgI BciVI BclI
BfrI BglII BsmI BspDI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRV EcoT22I FbaI FseI
HindIII HpaI KasI MfeI MluI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SfoI
SfuI SnaBI SphI SwaI Tru9I
XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 6967 GACGTC GACGT^C
Acc65I 3344 GGTACC G^GTACC
AccIII 6560 TCCGGA T^CCGGA
AflIII 814 ACACGT A^C[GA][CT]GT
AgeI 707 ACCGGT A^CCGGT
AhdI 5652 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 6272 GTGCAC G^TGCAC
ApaLI 6272 GTGCAC G^TGCAC
Asp718I 3344 GGTACC G^GTACC
AspEI 5652 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6996 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 7076 TGCGCA TGC^GCA
AvrII 958 CCTAGG C^CTAGG
BamHI 3567 GGATCC G^GATCC
BbrPI 815 CACGTG CAC^GTG
BbsI 4031 GAAGAC GAAGAC(2/6)
BlnI 958 CCTAGG C^CTAGG
BpuAI 4031 GAAGAC GAAGAC(2/6)
BsaBI 5352 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 6560 TCCGGA T^CCGGA
BsiEI 5021 CGACCG CG[GA][CT]^CG
BsiWI 5655 CGTACG C^GTACG
Bsp1407I 2542 TGTACA T^GTACA
BspEI 6560 TCCGGA T^CCGGA
BspHI 4497 TCATGA T^CATGA
BsrGI 2542 TGTACA T^GTACA
Eam1105I 5652 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 7076 TGCGCA TGC^GCA
HincII 711 GTCAAC GT[CT]^[GA]AC
HindII 711 GTCAAC GT[CT]^[GA]AC
KpnI 3344 GGTACC GGTAC^C
KspI 6159 CCGCGG CCGC^GG
MamI 5352 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 6560 TCCGGA T^CCGGA
NspI 6328 GCATGT [GA]CATG^[CT]
PaeR7I 3071 CTCGAG C^TCGAG
PflFI 6996 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 707 ACCGGT A^CCGGT
PmaCI 815 CACGTG CAC^GTG
PmlI 815 CACGTG CAC^GTG
RcaI 4497 TCATGA T^CATGA
SacII 6159 CCGCGG CCGC^GG
SbfI 4764 CCTGCAGG CCTGCA^GG
ScaI 6623 AGTACT AGT^ACT
SexAI 583 ACCAGGT A^CC[AT]GGT
SgrAI 630 CACCGGCG C[GA]^CCGG[CT]G
SpeI 3580 ACTAGT A^CTAGT
Sse8387I 4764 CCTGCAGG CCTGCA^GG
SspBI 2542 TGTACA T^GTACA
SspI 5459 AATATT AAT^ATT
Tth111I 6996 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3071 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 1200 GAATTC [GA]^AATT[CT]
2679 GAATTC
Aor51HI 5942 AGCGCT AGC^GCT
6941 AGCGCT
ApoI 1200 GAATTC [GA]^AATT[CT]
2679 GAATTC
Asp700I 1064 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2759 GAACCTCTTC
BsaAI 815 CACGTG [CT]AC^GT[GA]
5833 TACGTG
BsaWI 707 ACCGGT [AT]^CCGG[AT]
6560 TCCGGA
BsrDI 6783 CATTGC GCAATG(2/0)
6803 GCAATG
BstEII 4062 GGTGACC G^GT[ACGT]ACC
5238 GGTGACC
BstPI 4062 GGTGACC G^GT[ACGT]ACC
5238 GGTGACC
Eco47III 5942 AGCGCT AGC^GCT
6941 AGCGCT
EcoO65I 4062 GGTGACC G^GT[ACGT]ACC
5238 GGTGACC
EcoRI 1200 GAATTC G^AATTC
2679 GAATTC
SapI 6502 GAAGAGC GCTCTTC(1/4)
6220 GCTCTTC
XmnI 1064 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2759 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmaI SmlI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI