Restriction map for
pF1KB3077
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BamHI BanII BbeI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tsp45I Tsp509I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 763 GTCTAC GT^[AC][GT]AC
AflIII 530 ACATGT A^C[GA][CT]GT
AluI 42 AGCT AG^CT
Alw44I 473 GTGCAC G^TGCAC
ApaLI 473 GTGCAC G^TGCAC
AspHI 473 GTGCAC G[AT]GC[AT]^C
AspI 517 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 27 TGGCCA TGG^CCA
BanI 237 GGTGCC G^G[CT][GA]CC
BbrPI 470 CACGTG CAC^GTG
BbsI 3 GAAGAC GAAGAC(2/6)
BbvI 164 GCTGC GCAGC(8/12)
BcnI 146 CCCGG CC^[GC]GG
BfaI 298 CTAG C^TAG
BglI 635 GCCTGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 473 GTGCAC G[AGT]GC[ACT]^C
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaAI 470 CACGTG [CT]AC^GT[GA]
BsaJI 145 CCCCGG C^C[ACGT][ACGT]GG
BsiHKAI 473 GTGCAC G[AT]GC[AT]^C
BsiYI 140 CCCAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 140 CCCAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 424 GAGAC GTCTC(1/5)
Bsp1286I 473 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 527 TGTACA T^GTACA
BspHI 482 TCATGA T^CATGA
BspLU11I 530 ACATGT A^CATGT
BsrGI 527 TGTACA T^GTACA
BstAPI 49 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 328 GCGC GCG^C
EaeI 27 TGGCCA [CT]^GGCC[GA]
EarI 84 GAAGAG CTCTTC(1/4)
Fnu4HI 164 GCTGC GC^[ACGT]GC
HapII 147 CCGG C^CGG
HhaI 328 GCGC GCG^C
HinP1I 328 GCGC G^CGC
HpaII 147 CCGG C^CGG
ItaI 164 GCTGC GC^[ACGT]GC
Ksp632I 84 GAAGAG CTCTTC(1/4)
MaeI 298 CTAG C^TAG
MaeII 471 ACGT A^CGT
MaeIII 205 GTTAC ^GT[ACGT]AC
MluNI 27 TGGCCA TGG^CCA
MscI 27 TGGCCA TGG^CCA
MseI 305 TTAA T^TAA
MslI 754 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 31 CAGCGG C[AC]G^C[GT]G
MspI 147 CCGG C^CGG
NciI 146 CCCGG CC^[GC]GG
NdeI 415 CATATG CA^TATG
NspI 530 ACATGT [GA]CATG^[CT]
PflFI 517 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 470 CACGTG CAC^GTG
PmlI 470 CACGTG CAC^GTG
PstI 165 CTGCAG CTGCA^G
RcaI 482 TCATGA T^CATGA
SexAI 233 ACCAGGT A^CC[AT]GGT
SfcI 165 CTGCAG C^T[GA][CT]AG
SspBI 527 TGTACA T^GTACA
Tru9I 305 TTAA T^TAA
TseI 164 GCTGC G^C[AT]GC
Tth111I 517 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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AciI 33 GCGG CCGC(-3/-1)
150 GCGG
AfaI 528 GTAC GT^AC
534 GTAC
BpmI 599 CTCCAG CTGGAG(16/14)
59 CTGGAG
BsrI 685 ACTGG ACTGG(1/-1)
210 CCAGT
Cac8I 29 GCCAGC GC[ACGT]^[ACGT]GC
49 GCAGGC
DdeI 465 CTTAG C^T[ACGT]AG
543 CTTAG
HphI 774 GGTGA GGTGA(8/7)
595 TCACC
RsaI 528 GTAC GT^AC
534 GTAC
SfaNI 46 GATGC GCATC(5/9)
373 GATGC
TaqI 363 TCGA T^CGA
676 TCGA
TthHB8I 363 TCGA T^CGA
676 TCGA
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AvaII BssKI BstF5I BstNI Cfr13I
EcoRII FokI HaeIII HinfI MboII
MnlI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI TfiI TspRI