Restriction map for
pF1KB4401
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BclI BcnI BfaI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRV
EcoT22I FbaI FokI FseI FspI
HaeIII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MluI
MluNI MroI MscI MspA1I MspI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 312 GCGG CCGC(-3/-1)
AcsI 415 GAATTC [GA]^AATT[CT]
AflIII 434 ACATGT A^C[GA][CT]GT
AlwI 255 GATCC GGATC(4/5)
ApoI 415 GAATTC [GA]^AATT[CT]
AseI 157 ATTAAT AT^TAAT
AsnI 157 ATTAAT AT^TAAT
Asp700I 480 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 215 GGTCC G^G[AT]CC
BbsI 63 GAAGAC GAAGAC(2/6)
BbvCI 452 CCTCAGC CCTCAGC(-5/-2)
BciVI 547 GTATCC GTATCC(6/5)
BglII 182 AGATCT A^GATCT
BpuAI 63 GAAGAC GAAGAC(2/6)
BsaI 448 GAGACC GGTCTC(1/5)
BsmAI 448 GAGAC GTCTC(1/5)
BsmFI 188 GGGAC GGGAC(10/14)
BspLU11I 434 ACATGT A^CATGT
BspMI 196 GCAGGT ACCTGC(4/8)
BstYI 182 AGATCT [GA]^GATC[CT]
CfoI 242 GCGC GCG^C
Cfr13I 215 GGTCC G^G[ACGT]CC
EarI 484 CTCTTC CTCTTC(1/4)
EcoNI 562 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 415 GAATTC G^AATTC
EcoT14I 458 CCAAGG C^C[AT][AT]GG
FauI 312 GCGGG CCCGC(4/6)
HaeII 241 AGCGCC [GA]GCGC^[CT]
HhaI 242 GCGC GCG^C
HinP1I 242 GCGC G^CGC
Ksp632I 484 CTCTTC CTCTTC(1/4)
MaeII 67 ACGT A^CGT
MaeIII 230 GTTAC ^GT[ACGT]AC
MfeI 122 CAATTG C^AATTG
MflI 182 AGATCT [GA]^GATC[CT]
MunI 122 CAATTG C^AATTG
NlaIV 115 GGAGCC GG[ACGT]^[ACGT]CC
PleI 506 GACTC GAGTC(4/5)
PshBI 157 ATTAAT AT^TAAT
Sau96I 215 GGTCC G^G[ACGT]CC
SfaNI 578 GCATC GCATC(5/9)
SfcI 178 CTACAG C^T[GA][CT]AG
SphI 499 GCATGC GCATG^C
StyI 458 CCAAGG C^C[AT][AT]GG
XhoII 182 AGATCT [GA]^GATC[CT]
XmnI 480 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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BbvI 402 GCAGC GCAGC(8/12)
309 GCTGC
BpmI 445 CTGGAG CTGGAG(16/14)
563 CTGGAG
BsaJI 105 CCCAGG C^C[ACGT][ACGT]GG
458 CCAAGG
DpnII 183 GATC ^GATC
255 GATC
Fnu4HI 309 GCTGC GC^[ACGT]GC
402 GCAGC
ItaI 309 GCTGC GC^[ACGT]GC
402 GCAGC
MboI 183 GATC ^GATC
255 GATC
MslI 345 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
531 CACACTTGTG
NdeII 183 GATC ^GATC
255 GATC
NlaIII 435 CATG CATG^
500 CATG
NspI 434 ACATGT [GA]CATG^[CT]
499 GCATGC
Sau3AI 183 GATC ^GATC
255 GATC
TseI 309 GCTGC G^C[AT]GC
402 GCAGC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BsiYI BslI BssKI BstNI
Cac8I DdeI EcoRII HinfI HphI
MboII MnlI MseI MvaI ScrFI
TfiI Tru9I Tsp509I TspRI