Restriction map for
pF1KB6699
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DpnII DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MboI MfeI MflI MluI MroI
MseI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
Sau3AI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tsp509I Van91I XbaI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 471 GTAC GT^AC
AflIII 467 ACATGT A^C[GA][CT]GT
AgeI 712 ACCGGT A^CCGGT
AvaI 633 CTCGAG C^[CT]CG[GA]G
BalI 58 TGGCCA TGG^CCA
BsaI 237 GGTCTC GGTCTC(1/5)
BsaWI 712 ACCGGT [AT]^CCGG[AT]
BseRI 778 CTCCTC GAGGAG(10/8)
BsgI 178 CTGCAC GTGCAG(16/14)
BsoBI 633 CTCGAG C^[CT]CG[GA]G
BspLU11I 467 ACATGT A^CATGT
BspMI 728 ACCTGC ACCTGC(4/8)
BsrDI 141 GCAATG GCAATG(2/0)
BsrFI 712 ACCGGT [GA]^CCGG[CT]
BsrI 341 CCAGT ACTGG(1/-1)
BstEII 715 GGTAACC G^GT[ACGT]ACC
BstPI 715 GGTAACC G^GT[ACGT]ACC
BstXI 410 CCACGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 843 GCCTGC GC[ACGT]^[ACGT]GC
Cfr10I 712 ACCGGT [GA]^CCGG[CT]
EaeI 58 TGGCCA [CT]^GGCC[GA]
EcoO65I 715 GGTAACC G^GT[ACGT]ACC
HincII 529 GTCAAC GT[CT]^[GA]AC
HindII 529 GTCAAC GT[CT]^[GA]AC
MaeII 504 ACGT A^CGT
MluNI 58 TGGCCA TGG^CCA
MscI 58 TGGCCA TGG^CCA
MwoI 449 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PaeR7I 633 CTCGAG C^TCGAG
PinAI 712 ACCGGT A^CCGGT
RsaI 471 GTAC GT^AC
SacI 456 GAGCTC GAGCT^C
SbfI 844 CCTGCAGG CCTGCA^GG
SmlI 633 CTCGAG C^T[CT][GA]AG
Sse8387I 844 CCTGCAGG CCTGCA^GG
TaqI 634 TCGA T^CGA
TfiI 874 GATTC G^A[AT]TC
TthHB8I 634 TCGA T^CGA
XcmI 341 CCAGTGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 633 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
665 GAAACCCTTC
AspI 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
435 GACCTTGTC
BcnI 403 CCGGG CC^[GC]GG
452 CCCGG
BsmAI 127 GAGAC GTCTC(1/5)
238 GTCTC
BstF5I 135 CATCC GGATG(2/0)
774 CATCC
DdeI 393 CTCAG C^T[ACGT]AG
750 CTCAG
DsaI 410 CCACGG C^C[GA][CT]GG
573 CCACGG
EarI 781 CTCTTC CTCTTC(1/4)
124 GAAGAG
EcoT14I 242 CCAAGG C^C[AT][AT]GG
524 CCAAGG
FokI 135 CATCC GGATG(9/13)
774 CATCC
Ksp632I 781 CTCTTC CTCTTC(1/4)
124 GAAGAG
MslI 226 CACCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
727 CACCTGCATG
NciI 403 CCGGG CC^[GC]GG
452 CCCGG
NspI 467 ACATGT [GA]CATG^[CT]
732 GCATGT
PflFI 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
435 GACCTTGTC
PleI 610 GACTC GAGTC(4/5)
71 GAGTC
StyI 242 CCAAGG C^C[AT][AT]GG
524 CCAAGG
Tth111I 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
435 GACCTTGTC
XmnI 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
665 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI ApaI AspHI AvaII BanII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
Cfr13I DraII EcoO109I EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboII MnlI MspI
MvaI NlaIII NlaIV PpuMI PstI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI