Restriction map for
pF1KB9592
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccIII AciI AclI
AcyI AflII Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AvaI AvaII AviII
AvrII BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaHI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1286I BspDI BspEI
BspHI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
Bsu36I CelII Cfr13I ClaI CpoI
DraII DraIII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV FauI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MluI
MroI NaeI NarI NciI NgoMIV
NheI NotI NruI PacI PaeR7I
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SapI Sau96I SbfI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
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AccII 1685 CGCG CG^CG
AgeI 659 ACCGGT A^CCGGT
AhdI 1575 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 439 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1575 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 454 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1599 TGGCCA TGG^CCA
BamHI 711 GGATCC G^GATCC
BanI 898 GGTGCC G^G[CT][GA]CC
BbsI 1610 GAAGAC GAAGAC(2/6)
BciVI 1280 GTATCC GTATCC(6/5)
BclI 1355 TGATCA T^GATCA
BpuAI 1610 GAAGAC GAAGAC(2/6)
BsaBI 1411 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1573 GAGACC GGTCTC(1/5)
BsaWI 659 ACCGGT [AT]^CCGG[AT]
BseRI 309 GAGGAG GAGGAG(10/8)
BsgI 286 GTGCAG GTGCAG(16/14)
BsmBI 651 GAGACG CGTCTC(1/5)
BsmFI 1101 GGGAC GGGAC(10/14)
BspLU11I 1746 ACATGT A^CATGT
BspMI 373 GCAGGT ACCTGC(4/8)
BsrFI 659 ACCGGT [GA]^CCGG[CT]
BstBI 1471 TTCGAA TT^CGAA
BstUI 1685 CGCG CG^CG
BstXI 902 CCACCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 711 GGATCC [GA]^GATC[CT]
Cac8I 496 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 1691 GCGC GCG^C
Cfr10I 659 ACCGGT [GA]^CCGG[CT]
DraI 1170 TTTAAA TTT^AAA
EaeI 1599 TGGCCA [CT]^GGCC[GA]
Eam1105I 1575 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1578 CCATGG C^C[AT][AT]GG
EcoT22I 768 ATGCAT ATGCA^T
FbaI 1355 TGATCA T^GATCA
HaeIII 1600 GGCC GG^CC
HapII 660 CCGG C^CGG
HgaI 985 GCGTC GACGC(5/10)
HhaI 1691 GCGC GCG^C
HinP1I 1691 GCGC G^CGC
HincII 1547 GTCGAC GT[CT]^[GA]AC
HindII 1547 GTCGAC GT[CT]^[GA]AC
HpaII 660 CCGG C^CGG
MamI 1411 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1498 CAATTG C^AATTG
MflI 711 GGATCC [GA]^GATC[CT]
MluNI 1599 TGGCCA TGG^CCA
MscI 1599 TGGCCA TGG^CCA
MslI 408 CACCGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 179 CAGCTG C[AC]G^C[GT]G
MspI 660 CCGG C^CGG
MunI 1498 CAATTG C^AATTG
MvnI 1685 CGCG CG^CG
NcoI 1578 CCATGG C^CATGG
NdeI 1511 CATATG CA^TATG
NsiI 768 ATGCAT ATGCA^T
NspV 1471 TTCGAA TT^CGAA
PflFI 454 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 659 ACCGGT A^CCGGT
PstI 1230 CTGCAG CTGCA^G
PvuII 179 CAGCTG CAG^CTG
SalI 1547 GTCGAC G^TCGAC
ScaI 479 AGTACT AGT^ACT
SfuI 1471 TTCGAA TT^CGAA
SspI 1222 AATATT AAT^ATT
StyI 1578 CCATGG C^C[AT][AT]GG
Tth111I 454 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 711 GGATCC [GA]^GATC[CT]
XmnI 439 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1387 GTAGAC GT^[AC][GT]AC
1547 GTCGAC
AflIII 1551 ACGTGT A^C[GA][CT]GT
1746 ACATGT
BfaI 527 CTAG C^TAG
587 CTAG
Bsp1407I 360 TGTACA T^GTACA
510 TGTACA
BsrGI 360 TGTACA T^GTACA
510 TGTACA
BstF5I 1285 CATCC GGATG(2/0)
1585 CATCC
DsaI 1431 CCGTGG C^C[GA][CT]GG
1578 CCATGG
EcoRI 535 GAATTC G^AATTC
811 GAATTC
FokI 1285 CATCC GGATG(9/13)
1585 CATCC
HphI 407 TCACC GGTGA(8/7)
1064 TCACC
MaeI 527 CTAG C^TAG
587 CTAG
MseI 758 TTAA T^TAA
1171 TTAA
NspI 687 GCATGT [GA]CATG^[CT]
1746 ACATGT
SspBI 360 TGTACA T^GTACA
510 TGTACA
TfiI 121 GAATC G^A[AT]TC
1156 GAATC
Tru9I 758 TTAA T^TAA
1171 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BbvI BpmI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstNI
DdeI DpnII EcoRII Fnu4HI HinfI
ItaI MaeII MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI RsaI Sau3AI
ScrFI SfaNI SfcI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I