Restriction map for
pF1KB4125
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BbrPI BbsI
BbvCI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsiWI BsmBI BsoBI BspHI
BsrDI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraI DsaI Eam1105I Eco81I EcoO65I
EcoT14I FseI FspI HgaI KpnI
KspI MamI MluI MslI NaeI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SmlI SnaBI SphI Sse8387I StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1872 TCCGGA T^CCGGA
AgeI 1151 ACCGGT A^CCGGT
BalI 1941 TGGCCA TGG^CCA
BamHI 2415 GGATCC G^GATCC
BclI 803 TGATCA T^GATCA
BsaI 298 GAGACC GGTCTC(1/5)
BseAI 1872 TCCGGA T^CCGGA
BsiEI 293 CGGCCG CG[GA][CT]^CG
BsmI 1490 GAATGC GAATGC(1/-1)
BspDI 1825 ATCGAT AT^CGAT
BspEI 1872 TCCGGA T^CCGGA
BspMI 909 GCAGGT ACCTGC(4/8)
BsrBI 81 GAGCGG CCGCTC(-3/-3)
BstBI 2159 TTCGAA TT^CGAA
BstXI 824 CCAAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1825 ATCGAT AT^CGAT
DrdI 595 GACCACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 293 CGGCCG C^GGCCG
EclXI 293 CGGCCG C^GGCCG
Eco52I 293 CGGCCG C^GGCCG
EcoNI 999 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1616 GATATC GAT^ATC
FbaI 803 TGATCA T^GATCA
HindIII 782 AAGCTT A^AGCTT
HpaI 2697 GTTAAC GTT^AAC
MfeI 1063 CAATTG C^AATTG
MluNI 1941 TGGCCA TGG^CCA
MroI 1872 TCCGGA T^CCGGA
MscI 1941 TGGCCA TGG^CCA
MunI 1063 CAATTG C^AATTG
NdeI 1414 CATATG CA^TATG
NspV 2159 TTCGAA TT^CGAA
PflMI 1339 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1151 ACCGGT A^CCGGT
PpuMI 2013 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 688 CAGCTG CAG^CTG
SapI 2093 GCTCTTC GCTCTTC(1/4)
ScaI 1891 AGTACT AGT^ACT
SfuI 2159 TTCGAA TT^CGAA
SspI 1909 AATATT AAT^ATT
Van91I 1339 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2581 CCAAGTGGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 75 GGCGCC G[GA]^CG[CT]C
198 GGCGCC
Aor51HI 236 AGCGCT AGC^GCT
257 AGCGCT
Asp700I 781 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2992 GAAGAACTTC
BbeI 75 GGCGCC GGCGC^C
198 GGCGCC
BcgI 37 GCAGCGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1161 GCACCTCCTTCG
BsaHI 75 GGCGCC G[GA]^CG[CT]C
198 GGCGCC
BsmFI 313 GTCCC GGGAC(10/14)
2134 GTCCC
BspLU11I 709 ACATGT A^CATGT
724 ACATGT
BsrFI 330 GCCGGT [GA]^CCGG[CT]
1151 ACCGGT
BstYI 869 AGATCC [GA]^GATC[CT]
2415 GGATCC
Cfr10I 330 GCCGGT [GA]^CCGG[CT]
1151 ACCGGT
DraIII 1340 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2054 CACCTAGTG
EaeI 293 CGGCCG [CT]^GGCC[GA]
1941 TGGCCA
Eco47III 236 AGCGCT AGC^GCT
257 AGCGCT
EcoT22I 1078 ATGCAT ATGCA^T
1184 ATGCAT
Hin1I 75 GGCGCC G[GA]^CG[CT]C
198 GGCGCC
HincII 2697 GTTAAC GT[CT]^[GA]AC
2835 GTCAAC
HindII 2697 GTTAAC GT[CT]^[GA]AC
2835 GTCAAC
KasI 75 GGCGCC G^GCGCC
198 GGCGCC
MflI 869 AGATCC [GA]^GATC[CT]
2415 GGATCC
NarI 75 GGCGCC GG^CGCC
198 GGCGCC
NsiI 1078 ATGCAT ATGCA^T
1184 ATGCAT
SfcI 1666 CTACAG C^T[GA][CT]AG
2705 CTATAG
SfoI 75 GGCGCC GGC^GCC
198 GGCGCC
SpeI 1780 ACTAGT A^CTAGT
2029 ACTAGT
XhoII 869 AGATCC [GA]^GATC[CT]
2415 GGATCC
XmnI 781 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2992 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SspBI StuI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I