Restriction map for
pF1KB9405
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AocI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BbrPI BbvCI BcgI
BclI BfrI BglII BlnI BsaAI
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI MamI MfeI
MluI MluNI MscI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AhdI 1453 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 163 GTGCAC G^TGCAC
ApaI 81 GGGCCC GGGCC^C
ApaLI 163 GTGCAC G^TGCAC
AspEI 1453 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3 GGATCC G^GATCC
BbeI 129 GGCGCC GGCGC^C
BciVI 1269 GGATAC GTATCC(6/5)
BglI 252 GCCCATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 469 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 469 GCTCAGC GC^T[ACGT]AGC
BsaI 184 GAGACC GGTCTC(1/5)
BseRI 1402 GAGGAG GAGGAG(10/8)
BsgI 658 GTGCAG GTGCAG(16/14)
BspDI 577 ATCGAT AT^CGAT
BspHI 215 TCATGA T^CATGA
BspMI 523 GCAGGT ACCTGC(4/8)
BsrDI 985 GCAATG GCAATG(2/0)
BstXI 520 CCAGCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 469 GCTCAGC GC^T[ACGT]AGC
ClaI 577 ATCGAT AT^CGAT
EaeI 230 CGGCCA [CT]^GGCC[GA]
Eam1105I 1453 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 878 GCGTC GACGC(5/10)
HphI 1276 GGTGA GGTGA(8/7)
KasI 129 GGCGCC G^GCGCC
NarI 129 GGCGCC GG^CGCC
NspI 363 GCATGT [GA]CATG^[CT]
PaeR7I 1178 CTCGAG C^TCGAG
PflMI 764 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 215 TCATGA T^CATGA
SacI 371 GAGCTC GAGCT^C
SapI 201 GCTCTTC GCTCTTC(1/4)
ScaI 325 AGTACT AGT^ACT
SfoI 129 GGCGCC GGC^GCC
SmlI 1178 CTCGAG C^T[CT][GA]AG
TfiI 815 GAATC G^A[AT]TC
Tsp45I 634 GTCAC ^GT[GC]AC
Van91I 764 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1178 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 114 CGCG CG^CG
172 CGCG
AccIII 91 TCCGGA T^CCGGA
263 TCCGGA
AcsI 734 AAATTT [GA]^AATT[CT]
1014 AAATTC
AcyI 129 GGCGCC G[GA]^CG[CT]C
877 GGCGTC
AlwNI 335 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
925 CAGCAACTG
ApoI 734 AAATTT [GA]^AATT[CT]
1014 AAATTC
BbsI 486 GTCTTC GAAGAC(2/6)
1095 GTCTTC
BfaI 775 CTAG C^TAG
1483 CTAG
BpuAI 486 GTCTTC GAAGAC(2/6)
1095 GTCTTC
BsaHI 129 GGCGCC G[GA]^CG[CT]C
877 GGCGTC
BsaWI 91 TCCGGA [AT]^CCGG[AT]
263 TCCGGA
BseAI 91 TCCGGA T^CCGGA
263 TCCGGA
BspEI 91 TCCGGA T^CCGGA
263 TCCGGA
BsrFI 30 ACCGGC [GA]^CCGG[CT]
105 ACCGGC
BstUI 114 CGCG CG^CG
172 CGCG
BstYI 3 GGATCC [GA]^GATC[CT]
1146 AGATCC
CfoI 130 GCGC GCG^C
199 GCGC
Cfr10I 30 ACCGGC [GA]^CCGG[CT]
105 ACCGGC
DsaI 113 CCGCGG C^C[GA][CT]GG
171 CCGCGG
EarI 202 CTCTTC CTCTTC(1/4)
692 CTCTTC
HaeII 129 GGCGCC [GA]GCGC^[CT]
198 GGCGCT
HhaI 130 GCGC GCG^C
199 GCGC
Hin1I 129 GGCGCC G[GA]^CG[CT]C
877 GGCGTC
HinP1I 130 GCGC G^CGC
199 GCGC
HindIII 1427 AAGCTT A^AGCTT
1494 AAGCTT
Ksp632I 202 CTCTTC CTCTTC(1/4)
692 CTCTTC
KspI 113 CCGCGG CCGC^GG
171 CCGCGG
MaeI 775 CTAG C^TAG
1483 CTAG
MaeIII 634 GTCAC ^GT[ACGT]AC
1053 GTTAC
MflI 3 GGATCC [GA]^GATC[CT]
1146 AGATCC
MroI 91 TCCGGA T^CCGGA
263 TCCGGA
MspA1I 113 CCGCGG C[AC]G^C[GT]G
171 CCGCGG
MvnI 114 CGCG CG^CG
172 CGCG
PpuMI 599 AGGACCC [GA]G^G[AT]CC[CT]
687 AGGTCCT
SacII 113 CCGCGG CCGC^GG
171 CCGCGG
XhoII 3 GGATCC [GA]^GATC[CT]
1146 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XmaCI XmaI XmnI