################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 443 GTATAC GT^[AC][GT]AC AccII 72 CGCG CG^CG AciI 71 CCGC CCGC(-3/-1) AfaI 581 GTAC GT^AC AlwI 172 GATCC GGATC(4/5) BbvCI 93 CCTCAGC CCTCAGC(-5/-2) BcgI 82 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 26 CTGGAG CTGGAG(16/14) BsiYI 217 CCAACAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 217 CCAACAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspHI 374 TCATGA T^CATGA BsrDI 33 GCAATG GCAATG(2/0) BssKI 21 CCAGG ^CC[ACGT]GG Bst1107I 443 GTATAC GTA^TAC BstAPI 57 GCAACATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 144 GGATG GGATG(2/0) BstNI 21 CCAGG CC^[AT]GG BstUI 72 CGCG CG^CG BstZ17I 443 GTATAC GTA^TAC DdeI 94 CTCAG C^T[ACGT]AG DraI 642 TTTAAA TTT^AAA EarI 268 GAAGAG CTCTTC(1/4) EcoRII 21 CCAGG ^CC[AT]GG EcoT14I 99 CCTTGG C^C[AT][AT]GG FauI 70 CCCGC CCCGC(4/6) FokI 144 GGATG GGATG(9/13) HphI 321 TCACC GGTGA(8/7) Ksp632I 268 GAAGAG CTCTTC(1/4) MaeII 606 ACGT A^CGT MslI 280 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 21 CCAGG CC^[AT]GG MvnI 72 CGCG CG^CG PflMI 217 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 240 GAGTC GAGTC(4/5) RcaI 374 TCATGA T^CATGA RsaI 581 GTAC GT^AC SapI 268 GAAGAGC GCTCTTC(1/4) ScrFI 21 CCAGG CC^[ACGT]GG StyI 99 CCTTGG C^C[AT][AT]GG Van91I 217 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AluI 470 AGCT AG^CT 619 AGCT BsaJI 20 CCCAGG C^C[ACGT][ACGT]GG 99 CCTTGG BsrI 223 ACTGG ACTGG(1/-1) 326 CCAGT DpnII 131 GATC ^GATC 172 GATC EcoRI 370 GAATTC G^AATTC 625 GAATTC MboI 131 GATC ^GATC 172 GATC MwoI 10 GCTCCCGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 57 GCAACATCTGC NdeII 131 GATC ^GATC 172 GATC NlaIII 375 CATG CATG^ 417 CATG Sau3AI 131 GATC ^GATC 172 GATC SfaNI 499 GCATC GCATC(5/9) 601 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AcsI ApoI HinfI MboII MnlI MseI TfiI Tru9I Tsp509I TspRI