Restriction map for
pF1KB4456
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AlwI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BciVI BfrI BlnI BsaI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
DraI DraIII DrdI EcoRI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI KpnI MfeI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SalI SfuI SgrAI SnaBI SpeI
SspI SwaI TfiI Tru9I XbaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 2194 GACGTC GACGT^C
AflIII 1286 ACGCGT A^C[GA][CT]GT
Aor51HI 740 AGCGCT AGC^GCT
AspI 2177 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 868 CACGTG CAC^GTG
BcgI 1374 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2106 TGATCA T^GATCA
BfaI 961 CTAG C^TAG
BlpI 174 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 174 GCTGAGC GC^T[ACGT]AGC
BsaBI 1893 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 500 CGGCCG CG[GA][CT]^CG
BsmI 291 GAATGC GAATGC(1/-1)
BsrDI 1865 GCAATG GCAATG(2/0)
BssSI 301 CACGAG CACGAG(-5/-1)
CelII 174 GCTGAGC GC^T[ACGT]AGC
CpoI 662 CGGACCG CG^G[AT]CCG
EagI 500 CGGCCG C^GGCCG
EarI 2039 CTCTTC CTCTTC(1/4)
EclXI 500 CGGCCG C^GGCCG
Eco47III 740 AGCGCT AGC^GCT
Eco52I 500 CGGCCG C^GGCCG
FbaI 2106 TGATCA T^GATCA
Ksp632I 2039 CTCTTC CTCTTC(1/4)
MaeI 961 CTAG C^TAG
MamI 1893 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1286 ACGCGT A^CGCGT
NcoI 203 CCATGG C^CATGG
NspI 2125 GCATGC [GA]CATG^[CT]
PaeR7I 1372 CTCGAG C^TCGAG
PflFI 2177 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 868 CACGTG CAC^GTG
PmlI 868 CACGTG CAC^GTG
RsrII 662 CGGACCG CG^G[AT]CCG
SapI 2038 GCTCTTC GCTCTTC(1/4)
SbfI 762 CCTGCAGG CCTGCA^GG
SphI 2125 GCATGC GCATG^C
Sse8387I 762 CCTGCAGG CCTGCA^GG
StuI 1212 AGGCCT AGG^CCT
Tsp509I 1027 AATT ^AATT
Tth111I 2177 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1372 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AhdI 1564 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1780 GACCTGGTGTC
Alw44I 1231 GTGCAC G^TGCAC
1927 GTGCAC
AocI 1334 CCTCAGG CC^T[ACGT]AGG
2277 CCTCAGG
ApaLI 1231 GTGCAC G^TGCAC
1927 GTGCAC
AspEI 1564 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1780 GACCTGGTGTC
AviII 1193 TGCGCA TGC^GCA
1316 TGCGCA
BbvCI 1128 CCTCAGC CCTCAGC(-5/-2)
421 GCTGAGG
BglII 1238 AGATCT A^GATCT
1989 AGATCT
BsaAI 868 CACGTG [CT]AC^GT[GA]
1657 TACGTG
BsgI 1635 CTGCAC GTGCAG(16/14)
2086 CTGCAC
BsmAI 1363 GTCTC GTCTC(1/5)
1404 GTCTC
Bsp1407I 830 TGTACA T^GTACA
2100 TGTACA
BsrGI 830 TGTACA T^GTACA
2100 TGTACA
BstEII 1684 GGTAACC G^GT[ACGT]ACC
1875 GGTGACC
BstPI 1684 GGTAACC G^GT[ACGT]ACC
1875 GGTGACC
BstYI 1238 AGATCT [GA]^GATC[CT]
1989 AGATCT
Bsu36I 1334 CCTCAGG CC^T[ACGT]AGG
2277 CCTCAGG
Eam1105I 1564 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1780 GACCTGGTGTC
Eco81I 1334 CCTCAGG CC^T[ACGT]AGG
2277 CCTCAGG
EcoNI 1434 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2136 CCTCCCAGAGG
EcoO65I 1684 GGTAACC G^GT[ACGT]ACC
1875 GGTGACC
FspI 1193 TGCGCA TGC^GCA
1316 TGCGCA
KspI 585 CCGCGG CCGC^GG
666 CCGCGG
MflI 1238 AGATCT [GA]^GATC[CT]
1989 AGATCT
PstI 280 CTGCAG CTGCA^G
763 CTGCAG
PvuII 321 CAGCTG CAG^CTG
2159 CAGCTG
SacII 585 CCGCGG CCGC^GG
666 CCGCGG
ScaI 1820 AGTACT AGT^ACT
2238 AGTACT
SfiI 42 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
492 GGCCGTGCCGGCC
SspBI 830 TGTACA T^GTACA
2100 TGTACA
XcmI 1205 CCAAGAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1301 CCAACACACCACTGG
XhoII 1238 AGATCT [GA]^GATC[CT]
1989 AGATCT
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XmaCI XmaI