Restriction map for
pF1KB3214
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccI AclI AgeI Alw44I
ApaLI AscI AseI AsnI AspI
AvrII BalI BamHI BbeI BbsI
BbvCI BciVI BclI BlnI BpuAI
BsaBI BsiEI BsiWI BspDI BspLU11I
BspMI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I EcoRI
EcoRV FbaI FseI HindIII KasI
KspI MamI MfeI MluNI MscI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1503 GGTACC G^GTACC
AccIII 1777 TCCGGA T^CCGGA
AcsI 2016 GAATTT [GA]^AATT[CT]
AcyI 895 GACGCC G[GA]^CG[CT]C
AflII 1020 CTTAAG C^TTAAG
AflIII 668 ACGCGT A^C[GA][CT]GT
AhdI 1798 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 146 GGGCCC GGGCC^C
ApoI 2016 GAATTT [GA]^AATT[CT]
Asp718I 1503 GGTACC G^GTACC
AspEI 1798 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1186 CACGTG CAC^GTG
BcgI 468 CGAGAACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1020 CTTAAG C^TTAAG
BglII 1869 AGATCT A^GATCT
BlpI 2251 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2251 GCTGAGC GC^T[ACGT]AGC
BsaAI 1186 CACGTG [CT]AC^GT[GA]
BsaHI 895 GACGCC G[GA]^CG[CT]C
BseAI 1777 TCCGGA T^CCGGA
BsmI 1335 GCATTC GAATGC(1/-1)
BspEI 1777 TCCGGA T^CCGGA
BspHI 1941 TCATGA T^CATGA
BsrDI 2262 CATTGC GCAATG(2/0)
BssSI 457 CACGAG CACGAG(-5/-1)
BstEII 1135 GGTGACC G^GT[ACGT]ACC
BstPI 1135 GGTGACC G^GT[ACGT]ACC
BstXI 995 CCAGGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2251 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1798 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1135 GGTGACC G^GT[ACGT]ACC
Hin1I 895 GACGCC G[GA]^CG[CT]C
HincII 672 GTTAAC GT[CT]^[GA]AC
HindII 672 GTTAAC GT[CT]^[GA]AC
HpaI 672 GTTAAC GTT^AAC
KpnI 1503 GGTACC GGTAC^C
MluI 668 ACGCGT A^CGCGT
MroI 1777 TCCGGA T^CCGGA
NaeI 1408 GCCGGC GCC^GGC
NgoMIV 1408 GCCGGC G^CCGGC
PmaCI 1186 CACGTG CAC^GTG
PmlI 1186 CACGTG CAC^GTG
PpuMI 2421 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1941 TCATGA T^CATGA
SacI 654 GAGCTC GAGCT^C
SapI 1568 GCTCTTC GCTCTTC(1/4)
SmaI 2400 CCCGGG CCC^GGG
SspI 179 AATATT AAT^ATT
TaqI 920 TCGA T^CGA
TthHB8I 920 TCGA T^CGA
XmaCI 2400 CCCGGG C^CCGGG
XmaI 2400 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Aor51HI 1306 AGCGCT AGC^GCT
1982 AGCGCT
Asp700I 135 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1881 GAACGATTTC
AspHI 654 GAGCTC G[AT]GC[AT]^C
1323 GAGCAC
AviII 347 TGCGCA TGC^GCA
2278 TGCGCA
BfaI 785 CTAG C^TAG
2386 CTAG
BglI 148 GCCCACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
526 GCCAAGATGGC
BsaI 1540 GAGACC GGTCTC(1/5)
1025 GGTCTC
BsgI 57 CTGCAC GTGCAG(16/14)
1816 GTGCAG
BsiHKAI 654 GAGCTC G[AT]GC[AT]^C
1323 GAGCAC
BsmBI 505 GAGACG CGTCTC(1/5)
520 GAGACG
BsmFI 545 GGGAC GGGAC(10/14)
1416 GTCCC
Bsp1407I 1712 TGTACA T^GTACA
2488 TGTACA
BsrBI 908 GAGCGG CCGCTC(-3/-3)
1596 GAGCGG
BsrFI 1380 GCCGGT [GA]^CCGG[CT]
1408 GCCGGC
BsrGI 1712 TGTACA T^GTACA
2488 TGTACA
Cfr10I 1380 GCCGGT [GA]^CCGG[CT]
1408 GCCGGC
Eco47III 1306 AGCGCT AGC^GCT
1982 AGCGCT
EcoT22I 731 ATGCAT ATGCA^T
2091 ATGCAT
FauI 910 GCGGG CCCGC(4/6)
1598 GCGGG
FspI 347 TGCGCA TGC^GCA
2278 TGCGCA
HgaI 667 GACGC GACGC(5/10)
895 GACGC
MaeI 785 CTAG C^TAG
2386 CTAG
MslI 30 CATGTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1326 CACCTCGGTG
NcoI 1217 CCATGG C^CATGG
2204 CCATGG
NsiI 731 ATGCAT ATGCA^T
2091 ATGCAT
PvuII 968 CAGCTG CAG^CTG
1494 CAGCTG
SmlI 796 CTCAAG C^T[CT][GA]AG
1020 CTTAAG
SspBI 1712 TGTACA T^GTACA
2488 TGTACA
StuI 1433 AGGCCT AGG^CCT
1692 AGGCCT
XmnI 135 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1881 GAACGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII