Restriction map for
pF1KB4714
##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AvrII BamHI BbrPI BbvCI
BciVI BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII Eco81I EcoO65I
EcoRV EcoT22I FseI HgaI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacI SalI SapI
SbfI ScaI SfiI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 2361 AACGTT AA^CGTT
AcyI 1749 GGCGCC G[GA]^CG[CT]C
AflIII 2477 ACACGT A^C[GA][CT]GT
AhdI 1723 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1466 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 6 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1723 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2107 CCCGGG C^[CT]CG[GA]G
AviII 776 TGCGCA TGC^GCA
BbeI 1749 GGCGCC GGCGC^C
BcgI 376 CGAGCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2074 TACGTG [CT]AC^GT[GA]
BsaHI 1749 GGCGCC G[GA]^CG[CT]C
BsmBI 1927 GAGACG CGTCTC(1/5)
BsmI 2426 GAATGC GAATGC(1/-1)
BsoBI 2107 CCCGGG C^[CT]CG[GA]G
BssHII 1242 GCGCGC G^CGCGC
BstBI 2314 TTCGAA TT^CGAA
DrdI 1299 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 368 CGGCCG C^GGCCG
Eam1105I 1723 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 368 CGGCCG C^GGCCG
Eco52I 368 CGGCCG C^GGCCG
EcoRI 1120 GAATTC G^AATTC
FauI 1215 GCGGG CCCGC(4/6)
FspI 776 TGCGCA TGC^GCA
Hin1I 1749 GGCGCC G[GA]^CG[CT]C
KasI 1749 GGCGCC G^GCGCC
KspI 1869 CCGCGG CCGC^GG
NarI 1749 GGCGCC GG^CGCC
NcoI 1754 CCATGG C^CATGG
NspV 2314 TTCGAA TT^CGAA
PflMI 2013 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2110 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2361 AACGTT AA^CGTT
SacII 1869 CCGCGG CCGC^GG
SexAI 992 ACCTGGT A^CC[AT]GGT
SfoI 1749 GGCGCC GGC^GCC
SfuI 2314 TTCGAA TT^CGAA
SmaI 2107 CCCGGG CCC^GGG
SspI 2407 AATATT AAT^ATT
Van91I 2013 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1698 TCTAGA T^CTAGA
XmaCI 2107 CCCGGG C^CCGGG
XmaI 2107 CCCGGG C^CCGGG
XmnI 6 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2373 GTCTAC GT^[AC][GT]AC
2596 GTAGAC
AccII 1243 CGCG CG^CG
1870 CGCG
AflII 667 CTTAAG C^TTAAG
1045 CTTAAG
Aor51HI 422 AGCGCT AGC^GCT
524 AGCGCT
AspI 514 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1653 GACTGTGTC
BanI 453 GGTGCC G^G[CT][GA]CC
1749 GGCGCC
BclI 641 TGATCA T^GATCA
2283 TGATCA
BfrI 667 CTTAAG C^TTAAG
1045 CTTAAG
BglI 808 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1021 GCCACGCTGGC
BsrDI 291 CATTGC GCAATG(2/0)
1017 CATTGC
BssSI 787 CACGAG CACGAG(-5/-1)
478 CTCGTG
BstUI 1243 CGCG CG^CG
1870 CGCG
Eco47III 422 AGCGCT AGC^GCT
524 AGCGCT
EcoNI 930 CCTCAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1374 CCTGGGCCAGG
FbaI 641 TGATCA T^GATCA
2283 TGATCA
MvnI 1243 CGCG CG^CG
1870 CGCG
PflFI 514 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1653 GACTGTGTC
PleI 31 GAGTC GAGTC(4/5)
1921 GAGTC
StuI 203 AGGCCT AGG^CCT
2054 AGGCCT
TfiI 150 GATTC G^A[AT]TC
1003 GATTC
Tth111I 514 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1653 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AspHI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII