Restriction map for
pF1KB6519
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BcnI BfrI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstUI BstXI CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Tsp45I Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
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AccI 882 GTATAC GT^[AC][GT]AC
AlwI 798 GGATC GGATC(4/5)
AocI 58 CCTGAGG CC^T[ACGT]AGG
Asp700I 454 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 684 GAGCAC G[AT]GC[AT]^C
AvaI 398 CTCGAG C^[CT]CG[GA]G
AvaII 1002 GGACC G^G[AT]CC
BbsI 1058 GTCTTC GAAGAC(2/6)
BciVI 891 GGATAC GTATCC(6/5)
BclI 1047 TGATCA T^GATCA
BfaI 1043 CTAG C^TAG
BglII 372 AGATCT A^GATCT
BpuAI 1058 GTCTTC GAAGAC(2/6)
BsaBI 784 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 192 TCCGGT [AT]^CCGG[AT]
BseRI 199 GAGGAG GAGGAG(10/8)
BsiEI 101 CGGTCG CG[GA][CT]^CG
BsiHKAI 684 GAGCAC G[AT]GC[AT]^C
BsiYI 993 CCGAAGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 993 CCGAAGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 978 GGGAC GGGAC(10/14)
BsoBI 398 CTCGAG C^[CT]CG[GA]G
Bsp1407I 425 TGTACA T^GTACA
BspHI 519 TCATGA T^CATGA
BsrDI 874 CATTGC GCAATG(2/0)
BsrGI 425 TGTACA T^GTACA
Bst1107I 882 GTATAC GTA^TAC
BstEII 807 GGTTACC G^GT[ACGT]ACC
BstPI 807 GGTTACC G^GT[ACGT]ACC
BstYI 372 AGATCT [GA]^GATC[CT]
BstZ17I 882 GTATAC GTA^TAC
Bsu36I 58 CCTGAGG CC^T[ACGT]AGG
CfoI 206 GCGC GCG^C
Cfr13I 1002 GGACC G^G[ACGT]CC
DraI 732 TTTAAA TTT^AAA
EaeI 140 TGGCCG [CT]^GGCC[GA]
Eco81I 58 CCTGAGG CC^T[ACGT]AGG
EcoO65I 807 GGTTACC G^GT[ACGT]ACC
EcoT14I 209 CCTTGG C^C[AT][AT]GG
FauI 26 GCGGG CCCGC(4/6)
FbaI 1047 TGATCA T^GATCA
HaeII 205 AGCGCC [GA]GCGC^[CT]
HaeIII 141 GGCC GG^CC
HapII 193 CCGG C^CGG
HhaI 206 GCGC GCG^C
HinP1I 206 GCGC G^CGC
HincII 253 GTTGAC GT[CT]^[GA]AC
HindII 253 GTTGAC GT[CT]^[GA]AC
HpaII 193 CCGG C^CGG
HphI 63 GGTGA GGTGA(8/7)
MaeI 1043 CTAG C^TAG
MaeIII 808 GTTAC ^GT[ACGT]AC
MamI 784 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 372 AGATCT [GA]^GATC[CT]
MslI 634 CATGTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 193 CCGG C^CGG
NlaIV 750 GGCTCC GG[ACGT]^[ACGT]CC
PaeR7I 398 CTCGAG C^TCGAG
PleI 757 GAGTC GAGTC(4/5)
RcaI 519 TCATGA T^CATGA
Sau96I 1002 GGACC G^G[ACGT]CC
SfaNI 115 GATGC GCATC(5/9)
SmlI 398 CTCGAG C^T[CT][GA]AG
SphI 696 GCATGC GCATG^C
SspBI 425 TGTACA T^GTACA
StyI 209 CCTTGG C^C[AT][AT]GG
XcmI 830 CCAACAACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 398 CTCGAG C^TCGAG
XhoII 372 AGATCT [GA]^GATC[CT]
XmnI 454 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AfaI 426 GTAC GT^AC
602 GTAC
BglI 17 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
118 GCCGCAGTGGC
BpmI 752 CTCCAG CTGGAG(16/14)
19 CTGGAG
BsaJI 98 CCTCGG C^C[ACGT][ACGT]GG
209 CCTTGG
BstF5I 671 CATCC GGATG(2/0)
843 GGATG
FokI 671 CATCC GGATG(9/13)
843 GGATG
RsaI 426 GTAC GT^AC
602 GTAC
SfcI 448 CTACAG C^T[GA][CT]AG
764 CTACAG
TfiI 280 GATTC G^A[AT]TC
714 GAATC
Tsp509I 499 AATT ^AATT
569 AATT
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
Bsp1286I BsrI BssKI BstNI Cac8I
DdeI DpnII EarI EcoRII Fnu4HI
HinfI ItaI Ksp632I MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NspI Sau3AI ScrFI SspI
TaqI Tru9I TseI TspRI TthHB8I