Restriction map for
pF1KB5550
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BcnI BfrI BlnI
BpmI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoRV EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1008 GTCTAC GT^[AC][GT]AC
AcyI 280 GACGCC G[GA]^CG[CT]C
ApaI 890 GGGCCC GGGCC^C
BalI 734 TGGCCA TGG^CCA
BanI 1326 GGTGCC G^G[CT][GA]CC
BbrPI 303 CACGTG CAC^GTG
BciVI 1938 GGATAC GTATCC(6/5)
BclI 1221 TGATCA T^GATCA
BfaI 1682 CTAG C^TAG
BglI 883 GCCAAACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1346 AGATCT A^GATCT
BlpI 36 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC
BsaAI 303 CACGTG [CT]AC^GT[GA]
BsaHI 280 GACGCC G[GA]^CG[CT]C
BsaWI 1004 TCCGGT [AT]^CCGG[AT]
Bsp1407I 806 TGTACA T^GTACA
BspHI 1295 TCATGA T^CATGA
BsrGI 806 TGTACA T^GTACA
BssSI 1472 CTCGTG CACGAG(-5/-1)
BstBI 724 TTCGAA TT^CGAA
CelII 36 GCTCAGC GC^T[ACGT]AGC
FauI 48 GCGGG CCCGC(4/6)
FbaI 1221 TGATCA T^GATCA
Hin1I 280 GACGCC G[GA]^CG[CT]C
MaeI 1682 CTAG C^TAG
MluNI 734 TGGCCA TGG^CCA
MscI 734 TGGCCA TGG^CCA
NspV 724 TTCGAA TT^CGAA
PflMI 1150 CCATCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 303 CACGTG CAC^GTG
PmlI 303 CACGTG CAC^GTG
PpuMI 1478 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1295 TCATGA T^CATGA
SacI 985 GAGCTC GAGCT^C
SapI 982 GAAGAGC GCTCTTC(1/4)
SexAI 1238 ACCTGGT A^CC[AT]GGT
SfiI 882 GGCCAAACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 724 TTCGAA TT^CGAA
SmlI 1663 CTCAAG C^T[CT][GA]AG
SspBI 806 TGTACA T^GTACA
TspRI 1322 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1150 CCATCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 590 GGATC GGATC(4/5)
996 GGATC
AlwNI 1114 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1358 CAGCTTCTG
Asp700I 1072 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1873 GAAATTGTTC
AspHI 985 GAGCTC G[AT]GC[AT]^C
1210 GTGCTC
AvaI 262 CCCGAG C^[CT]CG[GA]G
968 CTCGGG
AvaII 107 GGACC G^G[AT]CC
1479 GGTCC
BanII 890 GGGCCC G[GA]GC[CT]^C
985 GAGCTC
BbvCI 469 GCTGAGG CCTCAGC(-5/-2)
1645 GCTGAGG
BcgI 23 CGATGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
83 GCACGGTGGTCG
BsaBI 748 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1569 GATCACAATC
BsaI 958 GAGACC GGTCTC(1/5)
1774 GAGACC
BsiEI 141 CGGCCG CG[GA][CT]^CG
297 CGGCCG
BsiHKAI 985 GAGCTC G[AT]GC[AT]^C
1210 GTGCTC
BsoBI 262 CCCGAG C^[CT]CG[GA]G
968 CTCGGG
BstEII 492 GGTTACC G^GT[ACGT]ACC
1234 GGTGACC
BstPI 492 GGTTACC G^GT[ACGT]ACC
1234 GGTGACC
BstYI 996 GGATCT [GA]^GATC[CT]
1346 AGATCT
DrdI 712 GACAATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
775 GACCAGCGTGTC
EagI 141 CGGCCG C^GGCCG
297 CGGCCG
EclXI 141 CGGCCG C^GGCCG
297 CGGCCG
Eco52I 141 CGGCCG C^GGCCG
297 CGGCCG
EcoO65I 492 GGTTACC G^GT[ACGT]ACC
1234 GGTGACC
EcoRI 1100 GAATTC G^AATTC
1457 GAATTC
HapII 864 CCGG C^CGG
1005 CCGG
HpaII 864 CCGG C^CGG
1005 CCGG
MamI 748 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1569 GATCACAATC
MflI 996 GGATCT [GA]^GATC[CT]
1346 AGATCT
MspI 864 CCGG C^CGG
1005 CCGG
NcoI 440 CCATGG C^CATGG
1778 CCATGG
PvuII 611 CAGCTG CAG^CTG
1535 CAGCTG
XhoII 996 GGATCT [GA]^GATC[CT]
1346 AGATCT
XmnI 1072 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1873 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BbsI BbvI BmyI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TthHB8I